0 2 An an DT 3 19 interferon-gamma interferon-gamma NN 20 30 activation activation NN 31 39 sequence sequence NN 40 48 mediates mediate VBZ 49 52 the the DT 53 68 transcriptional transcriptional JJ 69 79 regulation regulation NN 80 82 of of IN 83 86 the the DT 87 90 IgG IgG NNP 91 93 Fc Fc NNP 94 102 receptor receptor NN 103 107 type type NN 108 110 IC IC NNP 111 115 gene gene NN 116 118 by by IN 119 135 interferon-gamma interferon-gamma NN 135 136 . . . 138 148 Expression expression NN 149 151 of of IN 152 155 the the DT 156 159 IgG IgG NNP 160 162 Fc Fc NNP 163 171 receptor receptor NN 172 176 type type NN 177 178 I i CD 179 180 ( ( ( 180 182 Fc Fc NNP 183 188 gamma gamma NN 189 191 RI RI NNP 191 192 ) ) ) 193 195 on on IN 196 203 myeloid myeloid JJ 204 209 cells cell NNS 210 212 is be VBZ 213 225 dramatically dramatically RB 226 235 increased increase VBN 236 238 by by IN 239 248 treatment treatment NN 249 253 with with IN 254 270 interferon-gamma interferon-gamma NN 271 272 ( ( ( 272 281 IFN-gamma IFN-gamma NNP 281 282 ) ) ) 282 283 . . . 284 286 We we PRP 287 295 observed observe VBD 296 300 that that IN 301 303 Fc Fc NNP 304 309 gamma gamma NN 310 312 RI RI NNP 313 323 transcript transcript NN 324 330 levels level NNS 331 333 in in IN 334 348 monoblast-like monoblast-like JJ 349 353 U937 u937 NN 354 359 cells cell NNS 360 364 were be VBD 365 373 elevated elevate VBN 374 380 within within IN 381 382 3 3 CD 383 385 hr hr NN 386 389 and and CC 390 396 peaked peak VBD 397 399 12 12 CD 400 402 hr hr NN 403 408 after after IN 409 417 exposure exposure NN 418 420 to to TO 421 430 IFN-gamma IFN-gamma NNP 430 431 . . . 432 441 Treatment Treatment NNP 442 444 of of IN 445 449 U937 U937 NNP 450 454 with with IN 455 464 IFN-gamma IFN-gamma NNP 465 468 for for IN 469 470 9 9 CD 471 473 hr hr NN 474 476 in in IN 477 480 the the DT 481 489 presence presence NN 490 492 of of IN 493 506 cycloheximide cycloheximide NN 507 510 led lead VBD 511 513 to to TO 514 529 super-induction super-induction NN 530 532 of of IN 533 535 Fc Fc NNP 536 541 gamma gamma NN 542 544 RI RI NNP 545 555 expression expression NN 555 556 . . . 557 564 Nuclear nuclear JJ 565 571 run-on run-on JJ 572 580 analysis analysis NN 581 589 revealed reveal VBD 590 594 that that IN 595 598 the the DT 599 603 rate rate NN 604 606 of of IN 607 609 Fc Fc NNP 610 615 gamma gamma NN 616 618 RI RI NNP 619 632 transcription transcription NN 633 636 was be VBD 637 646 increased increase VBN 647 649 by by IN 650 659 IFN-gamma IFN-gamma NNP 659 660 . . . 661 668 Genomic genomic JJ 669 677 sequence sequence NN 678 686 upstream upstream JJ 687 689 of of IN 690 693 the the DT 694 696 Fc Fc NNP 697 702 gamma gamma NN 703 706 RIC RIC NNP 707 711 gene gene NN 712 715 was be VBD 716 722 cloned clone VBN 723 726 and and CC 727 736 subjected subject VBN 737 739 to to TO 740 746 primer primer NN 747 756 extension extension NN 757 765 analysis analysis NN 765 766 , , , 767 772 which which WDT 773 785 demonstrated demonstrate VBD 786 787 a a DT 788 794 single single JJ 795 808 transcription transcription NN 809 819 initiation initiation NN 820 824 site site NN 825 832 without without IN 833 834 a a DT 835 839 TATA TATA NNP 840 843 box box NN 843 844 . . . 845 854 Transient transient JJ 855 868 transfections transfection NNS 869 871 of of IN 872 875 CAT CAT NNP 876 884 reporter reporter NN 885 889 gene gene NN 890 900 constructs construct NNS 901 911 containing contain VBG 912 919 various various JJ 920 922 Fc Fc NNP 923 928 gamma gamma NN 929 932 RIC RIC NNP 933 941 promoter promoter NN 942 951 sequences sequence NNS 952 956 into into IN 957 961 U937 u937 NN 962 967 cells cell NNS 968 976 revealed reveal VBD 977 981 that that IN 982 983 a a DT 984 989 20-bp 20-bp JJ 990 996 region region NN 997 1008 surrounding surround VBG 1009 1012 the the DT 1013 1026 transcription transcription NN 1027 1032 start start NN 1033 1037 site site NN 1038 1039 ( ( ( 1039 1041 -7 -7 CD 1042 1044 to to TO 1045 1048 +13 +13 CD 1048 1049 ) ) ) 1050 1053 was be VBD 1054 1061 capable capable JJ 1062 1064 of of IN 1065 1074 mediating mediate VBG 1075 1088 transcription transcription NN 1089 1099 initiation initiation NN 1100 1103 and and CC 1104 1108 that that IN 1109 1111 an an DT 1112 1121 IFN-gamma IFN-gamma NNP 1122 1132 responsive responsive JJ 1133 1140 element element NN 1141 1142 ( ( ( 1142 1146 GIRE GIRE NNP 1146 1147 ) ) ) 1148 1151 was be VBD 1152 1159 present present JJ 1160 1166 within within IN 1167 1169 74 74 CD 1170 1172 bp bp NN 1173 1181 upstream upstream RB 1182 1184 of of IN 1185 1188 the the DT 1189 1202 transcription transcription NN 1203 1213 initiation initiation NN 1214 1218 site site NN 1218 1219 . . . 1220 1221 A a DT 1222 1227 17-bp 17-bp JJ 1228 1236 sequence sequence NN 1237 1244 between between IN 1245 1254 positions position NNS 1255 1258 -51 -51 CD 1259 1262 and and CC 1263 1266 -35 -35 CD 1267 1276 conferred confer VBD 1277 1286 IFN-gamma IFN-gamma NNP 1287 1301 responsiveness responsiveness NN 1302 1304 on on IN 1305 1306 a a DT 1307 1319 heterologous heterologous JJ 1320 1328 promoter promoter NN 1328 1329 . . . 1330 1345 Double-stranded double-stranded JJ 1346 1350 GIRE GIRE NNP 1351 1359 sequence sequence NN 1359 1360 , , , 1361 1364 but but CC 1365 1368 not not RB 1369 1370 a a DT 1371 1380 scrambled scramble VBN 1381 1389 sequence sequence NN 1389 1390 , , , 1391 1394 was be VBD 1395 1407 specifically specifically RB 1408 1413 bound bind VBN 1414 1416 by by IN 1417 1424 nuclear nuclear JJ 1425 1433 proteins protein NNS 1434 1438 from from IN 1439 1448 IFN-gamma IFN-gamma NNP 1449 1456 treated treated JJ 1457 1461 U937 u937 NN 1462 1467 cells cell NNS 1467 1468 . . . 1469 1472 Gel Gel NNP 1473 1478 shift shift NN 1479 1490 experiments experiment NNS 1491 1498 further further RB 1499 1505 showed show VBD 1506 1510 that that IN 1511 1514 the the DT 1515 1520 STAT1 stat1 NN 1521 1526 alpha alpha NN 1527 1534 protein protein NN 1535 1540 bound bind VBD 1541 1543 to to TO 1544 1547 the the DT 1548 1550 Fc Fc NNP 1551 1556 gamma gamma NN 1557 1560 RIC RIC NNP 1561 1565 GIRE GIRE NNP 1566 1568 in in IN 1569 1577 response response NN 1578 1580 to to TO 1581 1590 IFN-gamma IFN-gamma NNP 1591 1600 treatment treatment NN 1601 1603 of of IN 1604 1608 U937 u937 NN 1609 1614 cells cell NNS 1614 1615 . . . 1616 1619 The the DT 1620 1622 Fc Fc NNP 1623 1628 gamma gamma NN 1629 1632 RIC RIC NNP 1633 1637 GIRE GIRE NNP 1638 1640 is be VBZ 1641 1651 homologous homologous JJ 1652 1654 to to TO 1655 1658 the the DT 1659 1668 IFN-gamma IFN-gamma NNP 1669 1679 activation activation NN 1680 1688 sequence sequence NN 1689 1690 ( ( ( 1690 1693 GAS GAS NNP 1693 1694 ) ) ) 1695 1697 of of IN 1698 1701 the the DT 1702 1711 guanylate guanylate NN 1712 1719 binding binding NN 1720 1727 protein protein NN 1728 1731 and and CC 1732 1734 to to TO 1735 1736 X X NNP 1737 1740 box box NN 1741 1749 elements element NNS 1750 1752 of of IN 1753 1758 class class NN 1759 1761 II ii CD 1762 1765 MHC MHC NNP 1766 1771 genes gene NNS 1771 1772 . . . 1773 1776 Our our PRP$ 1777 1784 results result NNS 1785 1796 demonstrate demonstrate VBP 1797 1801 that that IN 1802 1817 transcriptional transcriptional JJ 1818 1828 regulation regulation NN 1829 1831 of of IN 1832 1835 the the DT 1836 1838 Fc Fc NNP 1839 1844 gamma gamma NN 1845 1848 RIC RIC NNP 1849 1853 gene gene NN 1854 1856 by by IN 1857 1866 IFN-gamma IFN-gamma NNP 1867 1875 involves involve VBZ 1876 1879 the the DT 1880 1887 binding binding NN 1888 1890 of of IN 1891 1896 STAT1 stat1 NN 1897 1902 alpha alpha NN 1903 1905 to to TO 1906 1907 a a DT 1908 1913 17-bp 17-bp JJ 1914 1917 GAS GAS NNP 1918 1926 homology homology NN 1927 1929 in in IN 1930 1933 the the DT 1934 1942 proximal proximal JJ 1943 1951 promoter promoter NN 1951 1952 . . .