0 3 The the DT 4 11 myeloid myeloid JJ 12 16 zinc zinc NN 17 23 finger finger NN 24 28 gene gene NN 28 29 , , , 30 35 MZF-1 MZF-1 NNP 35 36 , , , 37 46 regulates regulate VBZ 47 50 the the DT 51 55 CD34 cd34 NN 56 64 promoter promoter NN 65 67 in in FW 68 73 vitro vitro FW 73 74 . . . 76 81 MZF-1 MZF-1 NNP 82 84 is be VBZ 85 86 a a DT 87 91 C2H2 c2h2 NN 92 96 zinc zinc NN 97 103 finger finger NN 104 108 gene gene NN 109 117 encoding encode VBG 118 119 a a DT 120 128 putative putative JJ 129 144 transcriptional transcriptional JJ 145 154 regulator regulator NN 155 157 of of IN 158 165 myeloid myeloid JJ 166 181 differentiation differentiation NN 181 182 . . . 183 186 The the DT 187 192 MZF-1 mzf-1 NN 193 200 protein protein NN 201 209 contains contain VBZ 210 212 13 13 CD 213 217 C2H2 c2h2 NN 218 222 zinc zinc NN 223 230 fingers finger NNS 231 239 arranged arrange VBN 240 242 in in IN 243 252 bipartite bipartite JJ 253 256 DNA dna NN 257 264 binding binding NN 265 272 domains domain NNS 273 283 containing contain VBG 284 288 zinc zinc NN 289 296 fingers finger NNS 297 304 through through IN 305 306 4 4 CD 307 310 and and CC 310 311 , , , 312 314 in in IN 315 318 the the DT 319 335 carboxy-terminus carboxy-terminus NN 335 336 , , , 337 338 5 5 CD 339 346 through through IN 347 349 13 13 CD 349 350 . . . 351 353 We we PRP 354 364 previously previously RB 365 375 identified identify VBD 376 379 the the DT 380 383 DNA dna NN 384 393 consensus consensus NN 394 401 binding binding NN 402 406 site site NN 407 417 recognized recognize VBN 418 420 by by IN 421 424 the the DT 425 428 two two CD 429 432 DNA dna NN 433 440 binding binding NN 441 448 domains domain NNS 448 449 . . . 450 452 To to TO 453 459 assess assess VB 460 463 the the DT 464 477 transcription transcription NN 478 488 regulatory regulatory JJ 489 497 function function NN 498 500 of of IN 501 506 MZF-1 MZF-1 NNP 506 507 , , , 508 511 the the DT 512 523 full-length full-length JJ 524 529 MZF-1 mzf-1 NN 530 536 coding coding NN 537 543 region region NN 544 547 was be VBD 548 553 fused fuse VBN 554 556 to to TO 557 560 the the DT 561 564 DNA dna NN 565 572 binding binding NN 573 579 domain domain NN 580 582 of of IN 583 586 the the DT 587 592 yeast yeast NN 593 607 transactivator transactivator NN 608 612 GAL4 gal4 NN 612 613 . . . 614 617 The the DT 618 628 expression expression NN 629 635 vector vector NN 636 639 was be VBD 640 653 cotransfected cotransfecte VBN 654 658 with with IN 659 662 the the DT 663 678 chloramphenicol chloramphenicol NN 679 685 acetyl acetyl NN 686 697 transferase transferase NN 698 699 ( ( ( 699 702 CAT CAT NNP 702 703 ) ) ) 704 712 reporter reporter NN 713 717 gene gene NN 718 727 regulated regulate VBN 728 730 by by IN 731 734 the the DT 735 744 thymidine thymidine NN 745 751 kinase kinase NNP 752 760 promoter promoter NN 761 771 containing contain VBG 772 776 GAL4 GAL4 NNP 777 780 DNA DNA NNP 781 788 binding binding NN 789 794 sites site NNS 795 799 into into IN 800 803 NIH NIH NNP 804 807 3T3 3t3 NN 807 808 , , , 809 812 293 293 CD 812 813 , , , 814 818 K562 k562 NN 818 819 , , , 820 823 and and CC 824 830 Jurkat Jurkat NNP 831 835 cell cell NN 836 841 lines line NNS 841 842 . . . 843 848 MZF-1 MZF-1 NNP 849 858 represses repress VBZ 859 862 CAT CAT NNP 863 871 reporter reporter NN 872 876 gene gene NN 877 887 expression expression NN 888 891 via via IN 892 896 GAL4 gal4 NN 897 904 binding binding NN 905 910 sites site NNS 911 913 in in IN 914 917 the the DT 918 934 nonhematopoietic nonhematopoietic JJ 935 939 cell cell NN 940 945 lines line NNS 946 949 NIH NIH NNP 950 953 3T3 3t3 NN 954 957 and and CC 958 961 293 293 CD 961 962 . . . 963 965 In in IN 966 974 contrast contrast NN 974 975 , , , 976 981 MZF-1 MZF-1 NNP 982 991 activates activate VBZ 992 995 CAT CAT NNP 996 1004 reporter reporter NN 1005 1009 gene gene NN 1010 1020 expression expression NN 1021 1023 in in IN 1024 1027 the the DT 1028 1041 hematopoietic hematopoietic JJ 1042 1046 cell cell NN 1047 1052 lines line NNS 1053 1057 K562 K562 NNP 1058 1061 and and CC 1062 1068 Jurkat Jurkat NNP 1068 1069 . . . 1070 1073 The the DT 1074 1079 MZF-1 mzf-1 NN 1080 1087 binding binding NN 1088 1093 sites site NNS 1094 1097 are be VBP 1098 1105 present present JJ 1106 1108 in in IN 1109 1112 the the DT 1113 1122 promoters promoter NNS 1123 1125 of of IN 1126 1133 several several JJ 1134 1139 genes gene NNS 1140 1149 expressed express VBN 1150 1156 during during IN 1157 1164 myeloid myeloid JJ 1165 1180 differentiation differentiation NN 1180 1181 , , , 1182 1191 including include VBG 1192 1195 the the DT 1196 1200 CD34 cd34 NN 1201 1209 promoter promoter NN 1209 1210 . . . 1211 1216 MZF-1 mzf-1 NN 1217 1232 transcriptional transcriptional JJ 1233 1243 regulation regulation NN 1244 1246 of of IN 1247 1251 this this DT 1252 1267 physiologically physiologically RB 1268 1276 relevant relevant JJ 1277 1285 promoter promoter NN 1286 1289 was be VBD 1290 1298 assessed assess VBN 1299 1301 in in IN 1302 1306 both both CC 1307 1320 hematopoietic hematopoietic JJ 1321 1324 and and CC 1325 1341 nonhematopoietic nonhematopoietic JJ 1342 1346 cell cell NN 1347 1352 lines line NNS 1352 1353 . . . 1354 1365 Recombinant recombinant JJ 1366 1371 MZF-1 mzf-1 NN 1372 1379 protein protein NN 1380 1392 specifically specifically RB 1393 1398 binds bind VBZ 1399 1401 to to TO 1402 1405 the the DT 1406 1415 consensus consensus NN 1416 1423 binding binding NN 1424 1429 sites site NNS 1430 1432 in in IN 1433 1436 the the DT 1437 1441 CD34 cd34 NN 1442 1450 promoter promoter NN 1451 1453 in in IN 1454 1462 mobility mobility NN 1463 1468 shift shift NN 1469 1475 assays assay NNS 1475 1476 . . . 1477 1482 MZF-1 mzf-1 NN 1483 1493 expression expression NN 1494 1501 vectors vector NNS 1502 1506 were be VBD 1507 1520 cotransfected cotransfecte VBN 1521 1525 with with IN 1526 1529 the the DT 1530 1540 luciferase luciferase NN 1541 1549 reporter reporter NN 1550 1558 plasmids plasmid NNS 1559 1568 regulated regulate VBN 1569 1571 by by IN 1572 1575 the the DT 1576 1580 CD34 cd34 NN 1581 1589 promoter promoter NN 1590 1594 into into IN 1595 1599 both both CC 1600 1616 nonhematopoietic nonhematopoietic JJ 1617 1620 and and CC 1621 1634 hematopoietic hematopoietic JJ 1635 1639 cell cell NN 1640 1645 lines line NNS 1645 1646 . . . 1647 1649 As as IN 1650 1654 with with IN 1655 1658 the the DT 1659 1671 heterologous heterologous JJ 1672 1675 DNA dna NN 1676 1683 binding binding NN 1684 1690 domain domain NN 1690 1691 , , , 1692 1697 MZF-1 MZF-1 NNP 1698 1707 represses repress VBZ 1708 1716 reporter reporter NN 1717 1721 gene gene NN 1722 1732 expression expression NN 1733 1735 in in IN 1736 1752 nonhematopoietic nonhematopoietic JJ 1753 1757 cell cell NN 1758 1763 lines line NNS 1764 1767 and and CC 1768 1777 activates activate VBZ 1778 1788 expression expression NN 1789 1791 in in IN 1792 1805 hematopoietic hematopoietic JJ 1806 1810 cell cell NN 1811 1816 lines line NNS 1816 1817 . . . 1818 1828 Activation Activation NNP 1829 1831 of of IN 1832 1836 CD34 cd34 NN 1837 1847 expression expression NN 1848 1850 in in IN 1851 1864 hematopoietic hematopoietic JJ 1865 1869 cell cell NN 1870 1875 lines line NNS 1876 1878 is be VBZ 1879 1888 dependent dependent JJ 1889 1891 on on IN 1892 1895 the the DT 1896 1904 presence presence NN 1905 1907 of of IN 1908 1914 intact intact JJ 1915 1920 MZF-1 mzf-1 NN 1921 1928 binding binding NN 1929 1934 sites site NNS 1934 1935 . . . 1936 1939 The the DT 1940 1944 cell cell NN 1945 1958 type-specific type-specific JJ 1959 1969 regulation regulation NN 1970 1972 of of IN 1973 1976 the the DT 1977 1981 CD34 cd34 NN 1982 1990 promoter promoter NN 1991 1993 by by IN 1994 1999 MZF-1 MZF-1 NNP 2000 2008 suggests suggest VBZ 2009 2012 the the DT 2013 2021 presence presence NN 2022 2024 of of IN 2025 2040 tissue-specific tissue-specific JJ 2041 2060 regulators/adapters regulators/adapter NNS 2061 2063 or or CC 2064 2076 differential differential JJ 2077 2082 MZF-1 mzf-1 NN 2083 2096 modifications modification NNS 2097 2101 that that WDT 2102 2111 determine determine VBP 2112 2117 MZF-1 mzf-1 NN 2118 2133 transcriptional transcriptional JJ 2134 2144 regulatory regulatory JJ 2145 2153 function function NN 2153 2154 . . .