0	10	Regulation	regulation	NN
11	13	of	of	IN
14	27	transcription	transcription	NN
28	30	of	of	IN
31	34	the	the	DT
35	40	human	human	JJ
41	55	erythropoietin	erythropoietin	NN
56	64	receptor	receptor	NN
65	69	gene	gene	NN
70	72	by	by	IN
73	81	proteins	protein	NNS
82	89	binding	bind	VBG
90	92	to	to	TO
93	99	GATA-1	gata-1	NN
100	103	and	and	CC
104	107	Sp1	sp1	NN
108	114	motifs	motif	NNS
114	115	.	.	.

117	131	Erythropoietin	erythropoietin	NN
132	133	(	(	(
133	136	Epo	Epo	NNP
136	137	)	)	)
137	138	,	,	,
139	142	the	the	DT
143	150	primary	primary	JJ
151	160	regulator	regulator	NN
161	163	of	of	IN
164	167	the	the	DT
168	178	production	production	NN
179	181	of	of	IN
182	191	erythroid	erythroid	JJ
192	197	cells	cell	NNS
197	198	,	,	,
199	203	acts	act	VBZ
204	206	by	by	IN
207	214	binding	bind	VBG
215	217	to	to	TO
218	219	a	a	DT
220	224	cell	cell	NN
225	232	surface	surface	NN
233	241	receptor	receptor	NN
242	243	(	(	(
243	247	EpoR	EpoR	NNP
247	248	)	)	)
249	251	on	on	IN
252	261	erythroid	erythroid	JJ
262	273	progenitors	progenitor	NNS
273	274	.	.	.

275	277	We	we	PRP
278	282	used	use	VBD
283	291	deletion	deletion	NN
292	300	analysis	analysis	NN
301	304	and	and	CC
305	317	transfection	transfection	NN
318	324	assays	assay	NNS
325	329	with	with	IN
330	338	reporter	reporter	NN
339	343	gene	gene	NN
344	354	constructs	construct	NNS
355	357	to	to	TO
358	365	examine	examine	VB
366	369	the	the	DT
370	383	transcription	transcription	NN
384	391	control	control	NN
392	400	elements	element	NNS
401	403	in	in	IN
404	407	the	the	DT
408	409	5	5	CD
409	410	’	'	SYM
411	419	flanking	flanking	NN
420	426	region	region	NN
427	429	of	of	IN
430	433	the	the	DT
434	439	human	human	JJ
440	444	EpoR	epor	NN
445	449	gene	gene	NN
449	450	.	.	.

451	453	In	in	IN
454	463	erythroid	erythroid	JJ
464	469	cells	cell	NNS
470	474	most	most	JJS
475	477	of	of	IN
478	481	the	the	DT
482	495	transcription	transcription	NN
496	504	activity	activity	NN
505	508	was	be	VBD
509	518	contained	contain	VBN
519	521	in	in	IN
522	523	a	a	DT
524	527	150	150	CD
528	530	bp	bp	NN
531	539	promoter	promoter	NN
540	548	fragment	fragment	NN
549	553	with	with	IN
554	561	binding	bind	VBG
562	567	sites	site	NNS
568	571	for	for	IN
572	585	transcription	transcription	NN
586	593	factors	factor	NNS
594	597	AP2	ap2	NN
597	598	,	,	,
599	602	Sp1	Sp1	NNP
603	606	and	and	CC
607	610	the	the	DT
611	629	erythroid-specific	erythroid-specific	JJ
630	636	GATA-1	gata-1	NN
636	637	.	.	.

638	641	The	the	DT
642	645	150	150	CD
646	648	bp	bp	NN
649	654	hEpoR	hEpoR	NNP
655	663	promoter	promoter	NN
664	673	exhibited	exhibit	VBD
674	678	high	high	JJ
679	682	and	and	CC
683	686	low	low	JJ
687	695	activity	activity	NN
696	698	in	in	IN
699	708	erythroid	erythroid	JJ
709	714	OCIM1	ocim1	NN
715	718	and	and	CC
719	723	K562	k562	NN
724	729	cells	cell	NNS
729	730	,	,	,
731	743	respectively	respectively	RB
743	744	,	,	,
745	755	reflecting	reflect	VBG
756	759	the	the	DT
760	764	high	high	JJ
765	768	and	and	CC
769	772	low	low	JJ
773	779	levels	level	NNS
780	782	of	of	IN
783	795	constitutive	constitutive	JJ
796	801	hEpoR	hEpoR	NNP
802	812	expression	expression	NN
812	813	.	.	.

814	817	The	the	DT
818	824	GATA-1	gata-1	NN
825	828	and	and	CC
829	832	Sp1	sp1	NN
833	840	binding	binding	NN
841	846	sites	site	NNS
847	849	in	in	IN
850	854	this	this	DT
855	863	promoter	promoter	NN
864	871	lacking	lack	VBG
872	873	a	a	DT
874	878	TATA	TATA	NNP
879	887	sequence	sequence	NN
888	892	were	be	VBD
893	902	necessary	necessary	JJ
903	906	for	for	IN
907	908	a	a	DT
909	913	high	high	JJ
914	919	level	level	NN
920	922	of	of	IN
923	936	transcription	transcription	NN
937	947	activation	activation	NN
947	948	.	.	.

949	960	Protein-DNA	protein-dna	JJ
961	968	binding	binding	NN
969	976	studies	study	NNS
977	986	suggested	suggest	VBD
987	991	that	that	IN
992	995	Sp1	Sp1	NNP
996	999	and	and	CC
1000	1003	two	two	CD
1004	1009	other	other	JJ
1010	1016	CCGCCC	CCGCCC	NNP
1017	1024	binding	binding	NN
1025	1033	proteins	protein	NNS
1034	1038	from	from	IN
1039	1048	erythroid	erythroid	JJ
1049	1052	and	and	CC
1053	1066	non-erythroid	non-erythroid	JJ
1067	1072	cells	cell	NNS
1073	1078	could	could	MD
1079	1083	bind	bind	VB
1084	1086	to	to	TO
1087	1090	the	the	DT
1091	1094	Sp1	sp1	NN
1095	1102	binding	binding	NN
1103	1108	motif	motif	NN
1108	1109	.	.	.

1110	1112	By	by	IN
1113	1123	increasing	increase	VBG
1124	1130	GATA-1	gata-1	NN
1131	1137	levels	level	NNS
1138	1141	via	via	IN
1142	1157	co-transfection	co-transfection	NN
1157	1158	,	,	,
1159	1161	we	we	PRP
1162	1166	were	be	VBD
1167	1171	able	able	JJ
1172	1174	to	to	TO
1175	1188	transactivate	transactivate	VB
1189	1192	the	the	DT
1193	1198	hEpoR	hEpoR	NNP
1199	1207	promoter	promoter	NN
1208	1210	in	in	IN
1211	1215	K562	k562	NN
1216	1221	cells	cell	NNS
1222	1225	and	and	CC
1226	1239	non-erythroid	non-erythroid	JJ
1240	1245	cells	cell	NNS
1245	1246	,	,	,
1247	1250	but	but	CC
1251	1254	not	not	RB
1255	1257	in	in	IN
1258	1261	the	the	DT
1262	1268	highly	highly	RB
1269	1275	active	active	JJ
1276	1281	OCIM1	ocim1	NN
1282	1287	cells	cell	NNS
1287	1288	,	,	,
1289	1297	although	although	IN
1298	1304	GATA-1	gata-1	NN
1305	1309	mRNA	mrna	NN
1310	1316	levels	level	NNS
1317	1321	were	be	VBD
1322	1332	comparable	comparable	JJ
1333	1335	in	in	IN
1336	1341	OCIM1	OCIM1	NNP
1342	1345	and	and	CC
1346	1350	K562	k562	NN
1350	1351	.	.	.

1352	1365	Interestingly	interestingly	RB
1365	1366	,	,	,
1367	1371	when	when	WRB
1372	1374	we	we	PRP
1375	1382	mutated	mutate	VBD
1383	1386	the	the	DT
1387	1390	Sp1	sp1	NN
1391	1395	site	site	NN
1395	1396	,	,	,
1397	1406	resulting	result	VBG
1407	1409	in	in	IN
1410	1411	a	a	DT
1412	1418	marked	marked	JJ
1419	1427	decrease	decrease	NN
1428	1430	in	in	IN
1431	1436	hEpoR	hEpoR	NNP
1437	1445	promoter	promoter	NN
1446	1454	activity	activity	NN
1454	1455	,	,	,
1456	1458	we	we	PRP
1459	1464	could	could	MD
1465	1472	restore	restore	VB
1473	1488	transactivation	transactivation	NN
1489	1491	by	by	IN
1492	1502	increasing	increase	VBG
1503	1509	GATA-1	gata-1	NN
1510	1516	levels	level	NNS
1517	1519	in	in	IN
1520	1525	OCIM1	ocim1	NN
1526	1531	cells	cell	NNS
1531	1532	.	.	.

1533	1538	These	these	DT
1539	1543	data	datum	NNS
1544	1551	suggest	suggest	VBP
1552	1556	that	that	IN
1557	1562	while	while	IN
1563	1569	GATA-1	gata-1	NN
1570	1573	can	can	MD
1574	1587	transactivate	transactivate	VB
1588	1591	the	the	DT
1592	1596	EpoR	epor	NN
1597	1605	promoter	promoter	NN
1605	1606	,	,	,
1607	1610	the	the	DT
1611	1616	level	level	NN
1617	1619	of	of	IN
1620	1625	hEpoR	hEpoR	NNP
1626	1630	gene	gene	NN
1631	1641	expression	expression	NN
1642	1646	does	do	VBZ
1647	1650	not	not	RB
1651	1657	depend	depend	VB
1658	1660	on	on	IN
1661	1667	GATA-1	gata-1	NN
1668	1673	alone	alone	RB
1673	1674	.	.	.

1675	1681	Rather	rather	RB
1681	1682	,	,	,
1683	1688	hEpoR	hEpoR	NNP
1689	1702	transcription	transcription	NN
1703	1711	activity	activity	NN
1712	1719	depends	depend	VBZ
1720	1722	on	on	IN
1723	1735	coordination	coordination	NN
1736	1743	between	between	IN
1744	1747	Sp1	sp1	NN
1748	1751	and	and	CC
1752	1758	GATA-1	gata-1	NN
1759	1763	with	with	IN
1764	1769	other	other	JJ
1770	1783	cell-specific	cell-specific	JJ
1784	1791	factors	factor	NNS
1791	1792	,	,	,
1793	1802	including	include	VBG
1803	1811	possibly	possibly	RB
1812	1817	other	other	JJ
1818	1826	Sp1-like	sp1-like	JJ
1827	1834	binding	binding	NN
1835	1843	proteins	protein	NNS
1843	1844	,	,	,
1845	1847	to	to	TO
1848	1855	provide	provide	VB
1856	1860	high	high	JJ
1861	1866	level	level	NN
1866	1867	,	,	,
1868	1883	tissue-specific	tissue-specific	JJ
1884	1894	expression	expression	NN
1894	1895	.	.	.