0 4 TCL1 tcl1 NN 5 13 oncogene oncogene NN 14 24 activation activation NN 25 27 in in IN 28 39 preleukemic preleukemic JJ 40 41 T t NN 42 47 cells cell NNS 48 52 from from IN 53 54 a a DT 55 59 case case NN 60 62 of of IN 63 84 ataxia-telangiectasia ataxia-telangiectasia NN 84 85 . . . 87 90 The the DT 91 95 TCL1 tcl1 NN 96 104 oncogene oncogene NN 105 107 on on IN 108 113 human human JJ 114 124 chromosome chromosome NN 125 132 14q32.1 14q32.1 NN 133 135 is be VBZ 136 144 involved involve VBN 145 147 in in IN 148 158 chromosome chromosome NN 159 173 translocations translocation NNS 174 175 [ [ ( 175 194 t(14;14)(q11;q32.1) t(14;14)(q11;q32.1) NNP 195 198 and and CC 199 217 t(7;14)(q35;q32.1) t(7;14)(q35;q32.1) NN 217 218 ] ] ) 219 222 and and CC 223 233 inversions inversion NNS 234 235 [ [ ( 235 251 inv14(q11;q32.1) inv14(q11;q32.1) LS 251 252 ] ] ) 253 257 with with IN 258 261 TCR TCR NNP 262 272 alpha/beta alpha/beta NN 273 277 loci locus NNS 278 280 in in IN 281 287 T-cell t-cell NN 288 297 leukemias leukemia NNS 297 298 , , , 299 303 such such JJ 304 306 as as IN 307 323 T-prolymphocytic t-prolymphocytic JJ 324 325 ( ( ( 325 330 T-PLL T-PLL NNP 330 331 ) ) ) 331 332 . . . 333 335 It it PRP 336 338 is be VBZ 339 343 also also RB 344 352 involved involve VBN 353 355 in in IN 356 358 T- t- NN 359 364 acute acute JJ 365 369 and- and- CC 370 377 chronic chronic JJ 378 387 leukemias leukemia NNS 388 395 arising arise VBG 396 398 in in IN 399 404 cases case NNS 405 407 of of IN 408 429 ataxia-telangiectasia ataxia-telangiectasia NN 430 431 ( ( ( 431 433 AT AT NNP 433 434 ) ) ) 434 435 , , , 436 438 an an DT 439 455 immunodeficiency immunodeficiency NN 456 464 syndrome syndrome NN 464 465 . . . 466 473 Similar similar JJ 474 485 chromosomal chromosomal JJ 486 500 rearrangements rearrangement NNS 501 506 occur occur VBP 507 511 also also RB 512 514 in in IN 515 518 the the DT 519 527 clonally clonally RB 528 536 expanded expand VBN 537 538 T t NN 539 544 cells cell NNS 545 547 in in IN 548 550 AT AT NNP 551 559 patients patient NNS 560 566 before before IN 567 570 the the DT 571 581 appearance appearance NN 582 584 of of IN 585 588 the the DT 589 594 overt overt JJ 595 603 leukemia leukemia NN 603 604 . . . 605 607 We we PRP 608 612 have have VBP 613 621 analyzed analyze VBN 622 625 the the DT 626 636 expression expression NN 637 639 of of IN 640 644 TCL1 TCL1 NNP 645 649 mRNA mRNA NNP 650 653 and and CC 654 661 protein protein NN 662 664 in in IN 665 675 peripheral peripheral JJ 676 681 blood blood NN 682 693 lymphocytes lymphocyte NNS 694 695 ( ( ( 695 699 PBLs PBL NNP 699 700 ) ) ) 701 705 from from IN 706 710 four four CD 711 713 AT AT NNP 714 719 cases case NNS 720 723 and and CC 724 728 from from IN 729 736 healthy healthy JJ 737 745 controls control NNS 745 746 . . . 747 749 We we PRP 750 755 found find VBD 756 760 that that IN 761 764 the the DT 765 769 TCL1 tcl1 NN 770 774 gene gene NN 775 778 was be VBD 779 792 overexpressed overexpresse VBN 793 795 in in IN 796 799 the the DT 800 804 PBLs pbl NNS 805 807 of of IN 808 810 an an DT 811 813 AT AT NNP 814 821 patient patient NN 822 826 with with IN 827 828 a a DT 829 834 large large JJ 835 841 clonal clonal JJ 842 848 T-cell t-cell NN 849 859 population population NN 860 870 exhibiting exhibit VBG 871 874 the the DT 875 883 t(14;14) t(14;14) NN 884 897 translocation translocation NN 898 901 but but CC 902 905 not not RB 906 908 in in IN 909 912 the the DT 913 924 lymphocytes lymphocyte NNS 925 927 of of IN 928 931 the the DT 932 937 other other JJ 938 943 cases case NNS 943 944 . . . 945 957 Fluorescence Fluorescence NNP 958 960 in in FW 961 965 situ situ FW 966 979 hybridization hybridization NN 980 982 of of IN 983 986 the the DT 987 991 TCL1 tcl1 NN 992 999 genomic genomic JJ 1000 1005 locus locus NN 1006 1008 to to TO 1009 1019 lymphocyte lymphocyte NN 1020 1030 metaphases metaphase NNS 1031 1035 from from IN 1036 1039 the the DT 1040 1042 AT AT NNP 1043 1050 patient patient NN 1051 1055 with with IN 1056 1059 the the DT 1060 1066 T-cell t-cell NN 1067 1073 clonal clonal JJ 1074 1083 expansion expansion NN 1084 1090 showed show VBD 1091 1095 that that IN 1096 1099 the the DT 1100 1110 breakpoint breakpoint NN 1111 1113 of of IN 1114 1117 the the DT 1118 1126 t(14;14) t(14;14) NN 1127 1140 translocation translocation NN 1141 1145 lies lie VBZ 1146 1152 within within IN 1153 1156 the the DT 1157 1161 TCL1 tcl1 NN 1162 1167 locus locus NN 1168 1171 and and CC 1172 1174 is be VBZ 1175 1186 accompanied accompany VBN 1187 1189 by by IN 1190 1192 an an DT 1193 1201 inverted invert VBN 1202 1213 duplication duplication NN 1214 1216 of of IN 1217 1220 the the DT 1221 1227 distal distal JJ 1228 1232 part part NN 1233 1235 of of IN 1236 1246 chromosome chromosome NN 1247 1249 14 14 CD 1249 1250 . . . 1251 1256 These these DT 1257 1261 data datum NNS 1262 1270 indicate indicate VBP 1271 1275 that that IN 1276 1280 TCL1 tcl1 NN 1281 1283 is be VBZ 1284 1293 activated activate VBN 1294 1296 in in IN 1297 1308 preleukemic preleukemic JJ 1309 1315 clonal clonal JJ 1316 1321 cells cell NNS 1322 1324 as as IN 1325 1326 a a DT 1327 1338 consequence consequence NN 1339 1341 of of IN 1342 1352 chromosome chromosome NN 1353 1366 translocation translocation NN 1367 1376 involving involve VBG 1377 1386 sequences sequence NNS 1387 1391 from from IN 1392 1395 the the DT 1396 1399 TCR TCR NNP 1400 1405 locus locus NN 1406 1408 at at IN 1409 1414 14q11 14q11 NN 1414 1415 . . . 1416 1428 Deregulation deregulation NN 1429 1431 of of IN 1432 1436 TCL1 TCL1 NNP 1437 1439 is be VBZ 1440 1443 the the DT 1444 1449 first first JJ 1450 1455 event event NN 1456 1458 in in IN 1459 1462 the the DT 1463 1473 initiation initiation NN 1474 1476 of of IN 1477 1487 malignancy malignancy NN 1488 1490 in in IN 1491 1496 these these DT 1497 1502 types type NNS 1503 1505 of of IN 1506 1515 leukemias leukemia NNS 1516 1519 and and CC 1520 1530 represents represent VBZ 1531 1532 a a DT 1533 1542 potential potential JJ 1543 1547 tool tool NN 1548 1551 for for IN 1552 1560 clinical clinical JJ 1561 1571 evaluation evaluation NN 1571 1572 . . .