0 13 Interleukin-2 interleukin-2 NN 14 22 promoter promoter NN 23 31 activity activity NN 32 34 in in IN 35 47 Epstein-Barr epstein-barr JJ 48 65 virus-transformed virus-transformed JJ 66 67 B B NNP 68 79 lymphocytes lymphocyte NNS 80 82 is be VBZ 83 93 controlled control VBN 94 96 by by IN 97 104 nuclear nuclear JJ 105 115 factor-chi factor-chi NN 116 118 B. B. NNP 120 123 The the DT 124 134 regulation regulation NN 135 137 of of IN 138 149 interleukin interleukin NN 150 151 ( ( ( 151 156 IL)-2 il)-2 NN 157 161 gene gene NN 162 172 expression expression NN 173 176 has have VBZ 177 181 been be VBN 182 194 investigated investigate VBN 195 201 mainly mainly RB 202 204 in in IN 205 206 T t NN 207 218 lymphocytes lymphocyte NNS 218 219 , , , 220 223 the the DT 224 235 predominant predominant JJ 236 245 producers producer NNS 246 248 of of IN 249 253 IL-2 il-2 NN 253 254 . . . 255 262 However however RB 262 263 , , , 264 265 B B NNP 266 271 cells cell NNS 272 275 can can MD 276 280 also also RB 281 291 synthesize synthesize VB 292 296 IL-2 il-2 NN 296 297 . . . 298 300 In in IN 301 304 the the DT 305 312 present present JJ 313 318 study study NN 319 321 we we PRP 322 330 analyzed analyze VBD 331 334 the the DT 335 342 control control NN 343 345 of of IN 346 350 IL-2 il-2 NN 351 359 promoter promoter NN 360 368 activity activity NN 369 371 in in IN 372 384 Epstein-Barr epstein-barr JJ 385 390 virus virus NN 391 392 ( ( ( 392 408 EBV)-transformed ebv)-transformed JJ 409 410 B B NNP 411 415 cell cell NN 416 422 clones clone NNS 423 428 which which WDT 429 432 are be VBP 433 440 capable capable JJ 441 443 of of IN 444 453 secreting secrete VBG 454 458 IL-2 il-2 NN 459 461 at at IN 462 463 a a DT 464 467 low low JJ 468 473 level level NN 474 479 after after IN 480 491 stimulation stimulation NN 492 496 with with IN 497 504 phorbol phorbol NN 505 517 12-myristate 12-myristate NN 518 528 13-acetate 13-acetate NN 529 532 and and CC 533 536 the the DT 537 541 Ca2+ ca2+ NN 542 551 ionophore ionophore NN 552 561 ionomycin ionomycin NN 561 562 . . . 563 572 Transient transient JJ 573 586 transfections transfection NNS 587 592 using use VBG 593 601 reporter reporter NN 602 612 constructs construct NNS 613 617 with with IN 618 627 multiples multiple NNS 628 630 of of IN 631 644 transcription transcription NN 645 651 factor factor NN 652 659 binding binding NN 660 665 sites site NNS 666 670 from from IN 671 674 the the DT 675 679 IL-2 il-2 NN 680 688 promoter promoter NN 689 690 [ [ ( 690 696 distal distal JJ 697 704 nuclear nuclear JJ 705 711 factor factor NN 712 713 ( ( ( 713 719 NF)-AT NF)-AT NNP 719 720 , , , 721 729 proximal proximal JJ 730 735 NF-AT NF-AT NNP 735 736 , , , 737 749 AP-1/Octamer AP-1/Octamer NNP 750 751 ( ( ( 751 754 UPS UPS NNP 754 755 ) ) ) 756 758 or or CC 759 765 NF-chi NF-chi NNP 766 767 B B NNP 768 769 ( ( ( 769 773 TCEd TCEd NNP 773 774 ) ) ) 775 780 sites site NNS 780 781 ] ] ) 782 786 were be VBD 787 796 performed perform VBN 796 797 . . . 798 800 In in IN 801 816 EBV-transformed ebv-transformed JJ 817 818 B B NNP 819 825 clones clone NNS 825 826 , , , 827 830 the the DT 831 834 chi chi NN 835 836 B B NNP 837 841 site site NN 842 849 exerted exert VBD 850 853 the the DT 854 863 strongest strong JJS 864 873 inducible inducible JJ 874 882 activity activity NN 882 883 ; ; : 884 887 the the DT 888 893 NF-AT nf-at NN 894 901 binding binding NN 902 907 sites site NNS 908 914 showed show VBD 915 921 either either CC 922 924 no no DT 925 927 or or CC 928 932 only only RB 933 937 weak weak JJ 938 946 activity activity NN 947 955 compared compare VBN 956 958 to to TO 959 965 Jurkat jurkat NN 966 967 T t NN 968 973 cells cell NNS 973 974 . . . 975 977 An an DT 978 982 IL-2 il-2 NN 983 991 promoter promoter NN 992 999 bearing bear VBG 1000 1001 a a DT 1002 1011 defective defective JJ 1012 1018 NF-chi NF-chi NNP 1019 1020 B B NNP 1021 1025 site site NN 1026 1029 was be VBD 1030 1040 completely completely RB 1041 1049 inactive inactive JJ 1050 1052 in in IN 1053 1068 EBV-transformed ebv-transformed JJ 1069 1070 B B NNP 1071 1076 cells cell NNS 1076 1077 , , , 1078 1083 while while IN 1084 1086 it it PRP 1087 1092 still still RB 1093 1096 had have VBD 1097 1105 activity activity NN 1106 1108 in in IN 1109 1115 Jurkat Jurkat NNP 1116 1117 T t NN 1118 1123 cells cell NNS 1123 1124 . . . 1125 1127 In in IN 1128 1133 seven seven CD 1134 1139 EBV-B ebv-b NN 1140 1144 cell cell NN 1145 1151 clones clone NNS 1152 1154 or or CC 1155 1160 lines line NNS 1161 1170 differing differ VBG 1171 1173 in in IN 1174 1179 their their PRP$ 1180 1188 capacity capacity NN 1189 1191 to to TO 1192 1199 secrete secrete VB 1200 1204 IL-2 il-2 NN 1204 1205 , , , 1206 1209 the the DT 1210 1218 activity activity NN 1219 1221 of of IN 1222 1225 the the DT 1226 1230 IL-2 il-2 NN 1231 1239 promoter promoter NN 1240 1250 correlated correlate VBD 1251 1255 well well RB 1256 1260 with with IN 1261 1264 the the DT 1265 1271 status status NN 1272 1274 of of IN 1275 1279 IL-2 il-2 NN 1280 1289 secretion secretion NN 1289 1290 . . . 1291 1300 Similarly similarly RB 1300 1301 , , , 1302 1303 a a DT 1304 1309 human human JJ 1310 1326 immunodeficiency immunodeficiency NN 1327 1332 virus virus NN 1333 1341 promoter promoter NN 1341 1342 , , , 1343 1348 whose whose WP$ 1349 1357 activity activity NN 1358 1360 is be VBZ 1361 1371 controlled control VBN 1372 1379 through through IN 1380 1383 chi chi NN 1384 1385 B B NNP 1386 1393 factors factor NNS 1393 1394 , , , 1395 1398 was be VBD 1399 1404 found find VBN 1405 1407 to to TO 1408 1410 be be VB 1411 1417 active active JJ 1418 1420 in in IN 1421 1424 the the DT 1425 1429 IL-2 il-2 NN 1430 1439 producing produce VBG 1440 1445 EBV-B ebv-b NN 1446 1451 cells cell NNS 1451 1452 , , , 1453 1456 but but CC 1457 1465 inactive inactive JJ 1466 1468 in in IN 1469 1472 the the DT 1473 1491 non-IL-2-producing non-il-2-producing JJ 1492 1497 cells cell NNS 1497 1498 . . . 1499 1514 Electrophoretic electrophoretic JJ 1515 1523 mobility mobility NN 1524 1529 shift shift NN 1530 1536 assays assay NNS 1537 1542 using use VBG 1543 1550 protein protein NN 1551 1559 extracts extract NNS 1560 1564 from from IN 1565 1570 EBV-B ebv-b NN 1571 1576 cells cell NNS 1577 1580 and and CC 1581 1584 the the DT 1585 1589 IL-2 IL-2 NNP 1590 1596 NF-chi NF-chi NNP 1597 1598 B B NNP 1599 1604 probe probe NN 1605 1613 revealed reveal VBD 1614 1617 the the DT 1618 1630 constitutive constitutive JJ 1631 1641 generation generation NN 1642 1644 of of IN 1645 1648 chi chi NN 1649 1650 B B NNP 1651 1660 complexes complex NNS 1661 1663 in in IN 1664 1678 IL-2-secreting il-2-secreting NN 1679 1684 cells cell NNS 1685 1695 consisting consist VBG 1696 1702 mainly mainly RB 1703 1705 of of IN 1706 1719 heterodimeric heterodimeric JJ 1720 1727 p50/p65 p50/p65 NN 1728 1737 complexes complex NNS 1737 1738 . . . 1739 1740 A a DT 1741 1747 weaker weak JJR 1748 1751 chi chi NN 1752 1753 B B NNP 1754 1761 complex complex NN 1762 1771 formation formation NN 1772 1775 and and CC 1776 1792 faster-migrating faster-migrating JJ 1793 1802 complexes complex NNS 1803 1807 were be VBD 1808 1816 detected detect VBN 1817 1819 in in IN 1820 1838 non-IL-2-secreting non-il-2-secreting JJ 1839 1844 cells cell NNS 1844 1845 . . . 1846 1851 These these DT 1852 1859 results result NNS 1860 1871 demonstrate demonstrate VBP 1872 1876 that that IN 1877 1880 the the DT 1881 1885 IL-2 il-2 NN 1886 1892 NF-chi NF-chi NNP 1893 1894 B B NNP 1895 1899 site site NN 1900 1902 is be VBZ 1903 1916 indispensable indispensable JJ 1917 1920 for for IN 1921 1924 the the DT 1925 1933 activity activity NN 1934 1936 of of IN 1937 1940 the the DT 1941 1945 IL-2 il-2 NN 1946 1954 promoter promoter NN 1955 1957 in in IN 1958 1973 EBV-transformed ebv-transformed JJ 1974 1975 B B NNP 1976 1981 cells cell NNS 1981 1982 , , , 1983 1990 whereas whereas IN 1991 1996 other other JJ 1997 2010 transcription transcription NN 2011 2018 factors factor NNS 2019 2025 appear appear VBP 2026 2028 to to TO 2029 2031 be be VB 2032 2036 less less RBR 2037 2046 important important JJ 2047 2050 for for IN 2051 2055 IL-2 il-2 NN 2056 2066 expression expression NN 2067 2069 in in IN 2070 2075 these these DT 2076 2081 cells cell NNS 2081 2082 . . .