0 4 IL-1 il-1 NN 5 13 receptor receptor NN 14 17 and and CC 18 21 TCR TCR NNP 22 29 signals signal NNS 30 39 synergize synergize VBP 40 42 to to TO 43 51 activate activate VB 52 60 NF-kappa NF-kappa NNP 61 71 B-mediated b-mediated JJ 72 76 gene gene NN 77 90 transcription transcription NN 90 91 . . . 93 101 Previous previous JJ 102 109 studies study NNS 110 114 have have VBP 115 127 demonstrated demonstrate VBN 128 132 that that IN 133 137 IL-1 il-1 NN 138 146 receptor receptor NN 147 148 ( ( ( 148 155 IL-1R)- IL-1R)- NNP 156 159 and and CC 160 173 TCR-initiated tcr-initiated JJ 174 181 signals signal NNS 182 185 can can MD 186 194 interact interact VB 195 210 synergistically synergistically RB 211 213 to to TO 214 222 increase increase VB 223 226 the the DT 227 231 rate rate NN 232 234 of of IN 235 248 transcription transcription NN 249 251 of of IN 252 259 several several JJ 260 270 lymphokine lymphokine NN 271 274 and and CC 275 285 lymphokine lymphokine NN 286 294 receptor receptor NN 295 300 genes gene NNS 301 307 during during IN 308 311 the the DT 312 322 competence competence NN 323 328 phase phase NN 329 331 of of IN 332 335 the the DT 336 346 activation activation NN 347 354 program program NN 355 357 in in IN 358 359 T t NN 360 366 helper helper NN 367 378 lymphocytes lymphocyte NNS 378 379 . . . 380 382 In in IN 383 387 this this DT 388 394 report report NN 395 397 we we PRP 398 406 describe describe VBP 407 410 how how WRB 411 418 signals signal NNS 419 428 initiated initiate VBN 429 436 through through IN 437 440 the the DT 441 445 type type NN 446 447 I i CD 448 453 IL-1R il-1r NN 454 462 interact interact VBP 463 467 with with IN 468 475 signals signal NNS 476 480 from from IN 481 484 the the DT 485 492 antigen antigen NN 493 501 receptor receptor NN 502 504 to to TO 505 520 synergistically synergistically RB 521 528 augment augment VB 529 532 the the DT 533 548 transactivating transactivate VBG 549 559 properties property NNS 560 562 of of IN 563 571 NF-kappa NF-kappa NNP 572 574 B. B. NNP 575 578 The the DT 579 590 synergistic synergistic JJ 591 598 antigen antigen NN 599 607 receptor receptor NN 608 617 initiated initiate VBN 618 625 signals signal NNS 626 629 are be VBP 630 638 mediated mediate VBN 639 646 through through IN 647 654 protein protein NN 655 661 kinase kinase NNP 662 663 C C NNP 664 671 because because IN 672 676 they they PRP 677 680 can can MD 681 683 be be VB 684 692 mimicked mimic VBN 693 695 by by IN 696 699 the the DT 700 707 phorbol phorbol NN 708 713 ester ester NN 713 714 , , , 715 751 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 751 752 , , , 753 756 but but CC 757 760 not not RB 761 765 with with IN 766 773 calcium calcium NN 774 784 ionophores ionophore NNS 784 785 ; ; : 786 789 and and CC 790 793 are be VBP 794 807 staurosporine staurosporine NN 808 817 sensitive sensitive JJ 818 821 but but CC 822 834 cyclosporine cyclosporine NN 835 844 resistant resistant JJ 844 845 . . . 846 849 Gel Gel NNP 850 855 shift shift NN 856 864 analyses analysis NNS 865 876 demonstrate demonstrate VBP 877 881 that that IN 882 890 NF-kappa NF-kappa NNP 891 892 B B NNP 893 900 nuclear nuclear JJ 901 914 translocation translocation NN 915 917 is be VBZ 918 928 stimulated stimulate VBN 929 938 primarily primarily RB 939 941 by by IN 942 946 IL-1 il-1 NN 947 953 rather rather RB 954 958 than than IN 959 961 by by IN 962 969 antigen antigen NN 970 978 receptor receptor NN 979 986 signals signal NNS 986 987 . . . 988 995 Western western NN 996 1000 blot blot NN 1001 1004 and and CC 1005 1020 phosphorylation phosphorylation NN 1021 1029 analyses analysis NNS 1030 1041 demonstrate demonstrate VBP 1042 1046 that that IN 1047 1050 the the DT 1051 1062 synergistic synergistic JJ 1063 1069 effect effect NN 1070 1072 on on IN 1073 1081 NF-kappa NF-kappa NNP 1082 1083 B B NNP 1084 1094 functional functional JJ 1095 1103 activity activity NN 1104 1106 is be VBZ 1107 1118 independent independent JJ 1119 1121 of of IN 1122 1123 I i NN 1124 1129 kappa kappa NN 1130 1131 B B NNP 1132 1137 alpha alpha NN 1138 1139 ( ( ( 1139 1153 MAD3)-NF-kappa MAD3)-NF-kappa NNP 1154 1155 B B NNP 1156 1168 dissociation dissociation NN 1169 1171 in in IN 1172 1175 the the DT 1176 1183 cytosol cytosol NN 1184 1187 and and CC 1188 1190 is be VBZ 1191 1194 not not RB 1195 1205 associated associate VBN 1206 1210 with with IN 1211 1212 I i NN 1213 1218 kappa kappa NN 1219 1220 B B NNP 1221 1228 nuclear nuclear JJ 1229 1242 translocation translocation NN 1242 1243 . . . 1244 1247 The the DT 1248 1260 IL-1-induced il-1-induced JJ 1261 1269 NF-kappa NF-kappa NNP 1270 1271 B B NNP 1272 1275 DNA DNA NNP 1276 1283 nuclear nuclear JJ 1284 1296 localization localization NN 1297 1299 is be VBZ 1300 1309 transient transient JJ 1310 1313 and and CC 1314 1317 can can MD 1318 1320 be be VB 1321 1330 prolonged prolong VBN 1331 1337 either either CC 1338 1340 by by IN 1341 1343 an an DT 1344 1351 antigen antigen NN 1352 1370 receptor-initiated receptor-initiated JJ 1371 1377 signal signal NN 1378 1380 or or CC 1381 1383 by by IN 1384 1394 inhibiting inhibit VBG 1395 1402 protein protein NN 1403 1412 synthesis synthesis NN 1412 1413 . . . 1414 1419 These these DT 1420 1427 results result NNS 1428 1435 suggest suggest VBP 1436 1440 that that IN 1441 1445 IL-1 il-1 NN 1446 1453 induces induce VBZ 1454 1458 both both CC 1459 1467 NF-kappa NF-kappa NNP 1468 1469 B B NNP 1470 1477 nuclear nuclear JJ 1478 1491 translocation translocation NN 1492 1495 and and CC 1496 1499 the the DT 1500 1509 synthesis synthesis NN 1510 1512 of of IN 1513 1514 a a DT 1515 1525 protein(s) protein(s) NN 1526 1537 responsible responsible JJ 1538 1541 for for IN 1542 1553 terminating terminate VBG 1554 1562 NF-kappa NF-kappa NNP 1563 1568 B-DNA B-DNA NNP 1569 1580 interaction interaction NN 1581 1583 in in IN 1584 1587 the the DT 1588 1595 nucleus nucleus NN 1595 1596 . . . 1597 1604 Antigen antigen NN 1605 1613 receptor receptor NN 1614 1621 signals signal NNS 1622 1629 prolong prolong VBP 1630 1638 NF-kappa NF-kappa NNP 1639 1644 B-DNA B-DNA NNP 1645 1656 interaction interaction NN 1656 1657 , , , 1658 1666 probably probably RB 1667 1669 by by IN 1670 1682 functionally functionally RB 1683 1695 antagonizing antagonize VBG 1696 1699 the the DT 1700 1712 IL-1-induced il-1-induced JJ 1713 1722 synthesis synthesis NN 1723 1725 of of IN 1726 1727 a a DT 1728 1738 protein(s) protein(s) NN 1739 1750 responsible responsible JJ 1751 1754 for for IN 1755 1758 the the DT 1759 1768 transient transient JJ 1769 1777 NF-kappa NF-kappa NNP 1778 1783 B-DNA B-DNA NNP 1784 1795 interaction interaction NN 1796 1799 and and CC 1800 1812 consequently consequently RB 1813 1828 synergistically synergistically RB 1829 1836 enhance enhance VB 1837 1849 IL-1-induced il-1-induced JJ 1850 1858 NF-kappa NF-kappa NNP 1859 1870 B-dependent b-dependent JJ 1871 1875 gene gene NN 1876 1889 transcription transcription NN 1889 1890 . . .