0 9 Induction Induction NNP 10 12 of of IN 13 26 transcription transcription NN 27 34 factors factor NNS 35 37 in in IN 38 43 human human JJ 44 45 T t NN 46 57 lymphocytes lymphocyte NNS 58 60 by by IN 61 73 aspirin-like aspirin-like JJ 74 79 drugs drug NNS 79 80 . . . 82 94 Aspirin-like aspirin-like JJ 95 100 drugs drug NNS 101 102 ( ( ( 102 105 ALD ALD NNP 105 106 ) ) ) 107 113 induce induce VBP 114 121 calcium calcium NN 122 134 mobilization mobilization NN 134 135 , , , 136 138 an an DT 139 148 essential essential JJ 149 158 component component NN 159 161 of of IN 162 163 T t NN 164 168 cell cell NN 169 179 activation activation NN 179 180 , , , 181 184 but but CC 185 187 do do VBP 188 191 not not RB 192 198 induce induce VB 199 202 the the DT 203 215 biosynthesis biosynthesis NN 216 218 of of IN 219 223 IL-2 il-2 NN 223 224 . . . 225 227 To to TO 228 238 understand understand VB 239 242 the the DT 243 249 extent extent NN 250 252 to to TO 253 258 which which WDT 259 262 ALD ALD NNP 263 266 may may MD 267 272 mimic mimic VB 273 282 mitogenic mitogenic JJ 283 294 stimulation stimulation NN 294 295 , , , 296 298 we we PRP 299 306 studied study VBD 307 318 cytoplasmic cytoplasmic JJ 319 322 and and CC 323 330 nuclear nuclear JJ 331 340 signaling signaling NN 341 346 steps step NNS 347 349 in in IN 350 361 ALD-treated ald-treated JJ 362 363 T t NN 364 369 cells cell NNS 369 370 . . . 371 373 We we PRP 374 379 found find VBD 380 384 that that IN 385 388 ALD ald NN 389 395 induce induce VBP 396 397 a a DT 398 407 transient transient JJ 408 418 activation activation NN 419 421 of of IN 422 429 protein protein NN 430 436 kinase kinase NNP 437 438 ( ( ( 438 441 PKC PKC NNP 441 442 ) ) ) 443 446 but but CC 447 451 have have VBP 452 454 no no DT 455 461 effect effect NN 462 463 ( ( ( 463 465 in in IN 466 476 comparison comparison NN 477 479 to to TO 480 488 anti-CD3 anti-cd3 JJ 489 499 antibodies antibody NNS 499 500 ) ) ) 501 503 on on IN 504 511 protein protein NN 512 520 tyrosine tyrosine NN 521 536 phosphorylation phosphorylation NN 537 540 nor nor CC 541 543 on on IN 544 547 PCL PCL NNP 548 553 gamma gamma NN 554 555 1 1 CD 556 564 tyrosine tyrosine NN 565 580 phosphorylation phosphorylation NN 580 581 . . . 582 593 ALD-induced ald-induced JJ 594 601 calcium calcium NN 602 614 mobilization mobilization NN 615 618 and and CC 619 622 PKC PKC NNP 623 633 activation activation NN 634 637 are be VBP 638 649 independent independent JJ 650 652 of of IN 653 661 tyrosine tyrosine NN 662 669 protein protein NN 670 676 kinase kinase NNP 677 685 activity activity NN 686 688 as as IN 689 694 shown show VBN 695 697 by by IN 698 701 the the DT 702 706 lack lack NN 707 709 of of IN 710 716 effect effect NN 717 719 of of IN 720 730 herbimycin herbimycin NN 730 731 , , , 732 733 a a DT 734 750 tyrosine-protein tyrosine-protein JJ 751 766 kinase-specific kinase-specific JJ 767 776 inhibitor inhibitor NN 776 777 . . . 778 786 Although although IN 787 789 we we PRP 790 798 detected detect VBD 799 801 no no DT 802 806 IL-2 il-2 NN 807 811 mRNA mrna NN 812 814 in in IN 815 826 ALD-treated ald-treated JJ 827 832 cells cell NNS 832 833 , , , 834 837 the the DT 838 844 nuclei nucleus NNS 845 847 of of IN 848 853 these these DT 854 859 cells cell NNS 860 867 contain contain VBP 868 876 proteins protein NNS 877 884 capable capable JJ 885 887 of of IN 888 895 binding bind VBG 896 898 to to TO 899 904 three three CD 905 915 regulatory regulatory JJ 916 925 sequences sequence NNS 926 928 in in IN 929 932 the the DT 933 937 IL-2 il-2 NN 938 946 promoter promoter NN 947 953 region region NN 953 954 : : : 955 959 NFAT NFAT NNP 959 960 , , , 961 963 NF NF NNP 964 969 kappa kappa NN 970 971 B B NNP 971 972 , , , 973 976 and and CC 977 981 AP-1 ap-1 NN 981 982 . . . 983 988 These these DT 989 996 binding binding NN 997 1007 activities activity NNS 1008 1011 are be VBP 1012 1021 expressed express VBN 1022 1026 only only RB 1027 1029 in in IN 1030 1039 activated activate VBN 1040 1041 T t NN 1042 1047 cells cell NNS 1047 1048 . . . 1049 1052 The the DT 1053 1063 expression expression NN 1064 1066 of of IN 1067 1071 AP-1 ap-1 NN 1072 1080 depended depend VBD 1081 1083 on on IN 1084 1091 calcium calcium NN 1092 1104 mobilization mobilization NN 1105 1108 and and CC 1109 1112 PKC PKC NNP 1113 1123 activation activation NN 1123 1124 . . . 1125 1130 These these DT 1131 1135 data datum NNS 1136 1143 suggest suggest VBP 1144 1148 that that IN 1149 1152 ALD ald NN 1153 1158 cause cause VBP 1159 1168 transient transient JJ 1169 1172 but but CC 1173 1184 significant significant JJ 1185 1192 changes change NNS 1193 1195 in in IN 1196 1197 T t NN 1198 1202 cell cell NN 1203 1216 transmembrane transmembrane NN 1217 1226 signaling signaling NN 1226 1227 , , , 1228 1236 although although IN 1237 1241 some some DT 1242 1248 events event NNS 1249 1256 induced induce VBN 1257 1259 by by IN 1260 1271 stimulation stimulation NN 1272 1276 with with IN 1277 1285 anti-CD3 anti-cd3 JJ 1286 1296 antibodies antibody NNS 1297 1300 are be VBP 1301 1304 not not RB 1305 1312 induced induce VBN 1313 1315 by by IN 1316 1319 ALD ALD NNP 1319 1320 . . . 1321 1324 The the DT 1325 1331 signal signal NN 1332 1334 is be VBZ 1335 1346 transmitted transmit VBN 1347 1349 to to TO 1350 1353 the the DT 1354 1361 nucleus nucleus NN 1362 1365 and and CC 1366 1373 induces induce VBZ 1374 1385 DNA-binding dna-binding JJ 1386 1394 activity activity NN 1395 1397 by by IN 1398 1405 several several JJ 1406 1419 transcription transcription NN 1420 1427 factors factor NNS 1427 1428 . . . 1429 1436 However however RB 1436 1437 , , , 1438 1441 the the DT 1442 1445 ALD ALD NNP 1446 1454 stimulus stimulus NN 1455 1457 is be VBZ 1458 1461 not not RB 1462 1469 capable capable JJ 1470 1472 of of IN 1473 1480 causing cause VBG 1481 1489 complete complete JJ 1490 1491 T t NN 1492 1496 cell cell NN 1497 1507 activation activation NN 1507 1508 . . .