0 6 T-cell t-cell NN 7 17 functional functional JJ 18 25 regions region NNS 26 28 of of IN 29 32 the the DT 33 38 human human JJ 39 43 IL-3 il-3 NN 44 52 proximal proximal JJ 53 61 promoter promoter NN 61 62 . . . 64 67 The the DT 68 73 human human JJ 74 87 interleukin-3 interleukin-3 NN 88 89 ( ( ( 89 93 IL-3 IL-3 NNP 93 94 ) ) ) 95 99 gene gene NN 100 102 is be VBZ 103 112 expressed express VBN 113 119 almost almost RB 120 131 exclusively exclusively RB 132 134 in in IN 135 144 activated activate VBN 145 146 T t NN 147 152 cells cell NNS 152 153 . . . 154 157 Its its PRP$ 158 168 expression expression NN 169 171 is be VBZ 172 181 regulated regulate VBN 182 184 at at IN 185 189 both both CC 190 193 the the DT 194 209 transcriptional transcriptional JJ 210 213 and and CC 214 234 post-transcriptional post-transcriptional JJ 235 240 level level NN 240 241 . . . 242 244 We we PRP 245 249 have have VBP 250 260 previously previously RB 261 266 shown show VBN 267 271 that that IN 272 281 treatment treatment NN 282 284 of of IN 285 291 Jurkat Jurkat NNP 292 293 T t NN 294 299 cells cell NNS 300 304 with with IN 305 322 phytohemaglutinin phytohemaglutinin NN 323 324 ( ( ( 324 327 PHA PHA NNP 327 328 ) ) ) 329 332 and and CC 333 336 the the DT 337 344 phorbol phorbol NN 345 350 ester ester NN 350 351 , , , 352 355 PMA PMA NNP 355 356 , , , 357 366 activated activate VBD 367 380 transcription transcription NN 381 391 initiation initiation NN 392 396 from from IN 397 400 the the DT 401 405 IL-3 il-3 NN 406 410 gene gene NN 410 411 . . . 412 414 To to TO 415 421 define define VB 422 425 the the DT 426 433 regions region NNS 434 436 of of IN 437 440 the the DT 441 445 gene gene NN 446 454 required require VBN 455 458 for for IN 459 472 transcription transcription NN 473 483 activation activation NN 483 484 , , , 485 487 we we PRP 488 497 generated generate VBD 498 499 a a DT 500 506 series series NN 507 509 of of IN 510 518 reporter reporter NN 519 529 constructs construct NNS 530 540 containing contain VBG 541 550 different different JJ 551 558 regions region NNS 559 561 of of IN 562 565 the the DT 566 570 IL-3 il-3 NN 571 575 gene gene NN 576 577 5 5 CD 577 578 ’ ' SYM 579 582 and and CC 583 584 3 3 CD 584 585 ’ ' SYM 586 594 flanking flanking NN 595 604 sequences sequence NNS 604 605 . . . 606 610 Both both CC 611 619 positive positive JJ 620 623 and and CC 624 632 negative negative JJ 633 643 regulatory regulatory JJ 644 652 elements element NNS 653 657 were be VBD 658 668 identified identify VBN 669 671 in in IN 672 675 the the DT 676 684 proximal proximal JJ 685 686 5 5 CD 686 687 ’ ' SYM 688 696 flanking flanking NN 697 703 region region NN 704 706 of of IN 707 710 the the DT 711 715 IL-3 il-3 NN 716 720 gene gene NN 720 721 . . . 722 725 The the DT 726 734 promoter promoter NN 735 741 region region NN 742 749 between between IN 750 754 -173 -173 CD 755 758 and and CC 759 762 -60 -60 CD 763 772 contained contain VBD 773 776 the the DT 777 786 strongest strong JJS 787 797 activating activate VBG 798 806 elements element NNS 806 807 . . . 808 811 The the DT 812 825 transcription transcription NN 826 832 factor factor NN 833 837 AP-1 ap-1 NN 838 843 could could MD 844 848 bind bind VB 849 851 to to TO 852 856 this this DT 857 865 positive positive JJ 866 875 activator activator NN 876 882 region region NN 883 885 of of IN 886 889 the the DT 890 898 promoter promoter NN 898 899 . . . 900 902 We we PRP 903 907 also also RB 908 916 examined examine VBD 917 920 the the DT 921 929 function function NN 930 932 of of IN 933 936 the the DT 937 941 IL-3 il-3 NN 942 951 CK-1/CK-2 ck-1/ck-2 NN 952 960 elements element NNS 961 965 that that WDT 966 969 are be VBP 970 977 present present JJ 978 980 in in IN 981 985 many many JJ 986 994 cytokine cytokine NN 995 1000 genes gene NNS 1001 1004 and and CC 1005 1010 found find VBD 1011 1015 that that IN 1016 1020 they they PRP 1021 1026 acted act VBD 1027 1029 as as IN 1030 1031 a a DT 1032 1041 repressor repressor NN 1042 1044 of of IN 1045 1050 basal basal JJ 1051 1056 level level NN 1057 1067 expression expression NN 1068 1072 when when WRB 1073 1079 cloned clone VBN 1080 1088 upstream upstream RB 1089 1091 of of IN 1092 1093 a a DT 1094 1106 heterologous heterologous JJ 1107 1115 promoter promoter NN 1116 1119 but but CC 1120 1124 were be VBD 1125 1129 also also RB 1130 1139 inducible inducible JJ 1140 1142 by by IN 1143 1150 PMA/PHA PMA/PHA NNP 1150 1151 . . .