0 3 Two two CD 4 12 distinct distinct JJ 13 23 signalling signalling NN 24 32 pathways pathway NNS 33 36 are be VBP 37 45 involved involve VBN 46 48 in in IN 49 52 the the DT 53 60 control control NN 61 63 of of IN 64 67 the the DT 68 76 biphasic biphasic JJ 77 81 junB junb NN 82 95 transcription transcription NN 96 103 induced induce VBN 104 106 by by IN 107 120 interleukin-6 interleukin-6 NN 121 123 in in IN 124 127 the the DT 128 129 B B NNP 130 134 cell cell NN 135 144 hybridoma hybridoma NN 145 149 7TD1 7td1 NN 149 150 . . . 152 154 We we PRP 155 159 have have VBP 160 168 measured measure VBN 169 172 the the DT 173 178 level level NN 179 181 of of IN 182 186 junB junB NNP 187 191 mRNA mRNA NNP 192 194 in in IN 195 198 the the DT 199 200 B B NNP 201 210 hybridoma hybridoma NN 211 215 cell cell NN 216 220 line line NN 221 225 7TD1 7td1 NN 225 226 , , , 227 232 under under IN 233 246 interleukin-6 interleukin-6 NN 247 248 ( ( ( 248 252 IL-6 il-6 NN 252 253 ) ) ) 254 265 stimulation stimulation NN 265 266 . . . 267 271 IL-6 IL-6 NNP 272 281 increases increase VBZ 282 286 junB junb NN 287 291 mRNA mRNA NNP 292 294 in in IN 295 296 a a DT 297 305 biphasic biphasic JJ 306 313 fashion fashion NN 313 314 . . . 315 318 The the DT 319 324 first first JJ 325 338 early-induced early-induced JJ 339 343 peak peak NN 344 347 was be VBD 348 357 transient transient JJ 358 361 and and CC 362 368 likely likely RB 369 380 corresponds correspond VBZ 381 383 to to TO 384 387 the the DT 388 392 well well RB 393 403 documented document VBN 404 411 typical typical JJ 412 416 junB junB NNP 417 421 mRNA mRNA NNP 421 422 , , , 423 433 stimulated stimulate VBN 434 436 in in IN 437 445 response response NN 446 448 to to TO 449 457 numerous numerous JJ 458 464 growth growth NN 465 472 factors factor NNS 472 473 , , , 474 483 including include VBG 484 488 IL-6 il-6 NN 488 489 . . . 490 492 At at IN 493 501 variance variance NN 501 502 , , , 503 506 the the DT 507 513 second second JJ 514 518 peak peak NN 519 524 which which WDT 525 528 has have VBZ 529 534 never never RB 535 539 been be VBN 540 548 reported report VBN 549 559 previously previously RB 559 560 , , , 561 567 lasted last VBD 568 575 several several JJ 576 581 hours hour NNS 581 582 . . . 583 585 As as IN 586 587 a a DT 588 599 consequence consequence NN 600 602 of of IN 603 606 its its PRP$ 607 613 effect effect NN 614 616 on on IN 617 621 junB junB NNP 622 626 mRNA mRNA NNP 626 627 , , , 628 632 IL-6 IL-6 NNP 633 643 stimulated stimulate VBD 643 644 , , , 645 647 in in IN 648 649 a a DT 650 658 biphasic biphasic JJ 659 666 fashion fashion NN 666 667 , , , 668 671 the the DT 672 679 nuclear nuclear JJ 680 692 accumulation accumulation NN 693 695 of of IN 696 699 the the DT 700 704 JunB JunB NNP 705 712 protein protein NN 712 713 . . . 714 716 In in IN 717 721 this this DT 722 727 study study NN 727 728 , , , 729 731 we we PRP 732 744 demonstrated demonstrate VBD 745 749 that that IN 750 754 IL-6 IL-6 NNP 755 765 regulation regulation NN 766 774 occurred occur VBD 775 786 exclusively exclusively RB 787 789 at at IN 790 793 the the DT 794 809 transcriptional transcriptional JJ 810 815 level level NN 816 819 and and CC 820 824 that that IN 825 828 the the DT 829 836 bimodal bimodal JJ 837 845 increase increase NN 846 848 of of IN 849 853 junB junB NNP 854 858 mRNA mRNA NNP 859 862 and and CC 863 867 JunB JunB NNP 868 875 protein protein NN 876 879 can can MD 880 882 be be VB 883 892 accounted account VBN 893 896 for for IN 897 899 by by IN 900 901 a a DT 902 910 biphasic biphasic JJ 911 922 stimulation stimulation NN 923 925 of of IN 926 930 junB junb NN 931 944 transcription transcription NN 944 945 . . . 946 957 Furthermore furthermore RB 957 958 , , , 959 962 our our PRP$ 963 967 data datum NNS 968 973 point point VBP 974 976 to to TO 977 980 two two CD 981 986 major major JJ 987 998 differences difference NNS 999 1006 between between IN 1007 1010 the the DT 1011 1020 mechanism mechanism NN 1021 1023 of of IN 1024 1031 control control NN 1032 1034 of of IN 1035 1038 the the DT 1039 1044 early early JJ 1045 1048 and and CC 1049 1052 the the DT 1053 1057 late late JJ 1058 1070 IL-6-induced il-6-induced JJ 1071 1075 junB junb NN 1076 1089 transcription transcription NN 1090 1095 waves wave NNS 1095 1096 . . . 1097 1102 First first RB 1102 1103 , , , 1104 1117 cycloheximide cycloheximide NN 1118 1126 strongly strongly RB 1127 1138 potentiated potentiate VBD 1139 1142 the the DT 1143 1156 transcription transcription NN 1157 1159 of of IN 1160 1163 the the DT 1164 1170 second second JJ 1171 1175 wave wave NN 1175 1176 , , , 1177 1184 whereas whereas IN 1185 1187 it it PRP 1188 1194 failed fail VBD 1195 1197 to to TO 1198 1204 affect affect VB 1205 1208 the the DT 1209 1222 early-induced early-induced JJ 1223 1228 burst burst NN 1228 1229 . . . 1230 1236 Second second RB 1236 1237 , , , 1238 1248 tyrphostin tyrphostin NN 1248 1249 , , , 1250 1251 a a DT 1252 1260 tyrosine tyrosine NN 1261 1267 kinase kinase NNP 1268 1277 inhibitor inhibitor NN 1277 1278 , , , 1279 1287 impaired impair VBD 1288 1291 the the DT 1292 1302 expression expression NN 1303 1305 of of IN 1306 1309 the the DT 1310 1315 first first JJ 1316 1319 but but CC 1320 1323 not not RB 1324 1327 the the DT 1328 1334 second second JJ 1335 1339 junB junb NN 1340 1344 mRNA mrna NN 1345 1349 peak peak NN 1349 1350 . . . 1351 1361 Conversely conversely RB 1361 1362 , , , 1363 1372 genistein genistein NN 1372 1373 , , , 1374 1381 another another DT 1382 1390 tyrosine tyrosine NN 1391 1397 kinase kinase NNP 1398 1407 inhibitor inhibitor NN 1407 1408 , , , 1409 1416 totally totally RB 1417 1426 abolished abolish VBD 1427 1430 the the DT 1431 1441 expression expression NN 1442 1444 of of IN 1445 1448 the the DT 1449 1455 second second JJ 1456 1460 peak peak NN 1461 1463 of of IN 1464 1468 junB junB NNP 1469 1473 mRNA mRNA NNP 1474 1481 whereas whereas IN 1482 1484 it it PRP 1485 1488 did do VBD 1489 1492 not not RB 1493 1499 affect affect VB 1500 1503 the the DT 1504 1514 expression expression NN 1515 1517 of of IN 1518 1521 the the DT 1522 1527 first first JJ 1528 1532 peak peak NN 1532 1533 . . . 1534 1544 Altogether altogether RB 1545 1550 these these DT 1551 1555 data datum NNS 1556 1564 indicate indicate VBP 1565 1569 that that IN 1569 1570 , , , 1571 1573 in in IN 1574 1578 7TD1 7td1 NN 1579 1584 cells cell NNS 1584 1585 , , , 1586 1590 IL-6 IL-6 NNP 1591 1599 controls control VBZ 1600 1604 junB junb NN 1605 1618 transcription transcription NN 1619 1621 in in IN 1622 1623 a a DT 1624 1632 biphasic biphasic JJ 1633 1640 fashion fashion NN 1641 1643 by by IN 1644 1649 means mean NNS 1650 1652 of of IN 1653 1656 two two CD 1657 1665 separate separate JJ 1666 1678 transduction transduction NN 1679 1687 pathways pathway NNS 1687 1688 . . .