0 14 Overexpression overexpression NN 15 17 of of IN 18 25 protein protein NN 26 32 kinase kinase NNP 33 39 C-zeta C-zeta NNP 40 50 stimulates stimulate VBZ 51 59 leukemic leukemic JJ 60 64 cell cell NN 65 80 differentiation differentiation NN 80 81 . . . 83 84 A a DT 85 93 function function NN 94 97 for for IN 98 105 protein protein NN 106 112 kinase kinase NNP 113 119 C-zeta C-zeta NNP 120 121 ( ( ( 121 129 PKC-zeta PKC-zeta NNP 129 130 ) ) ) 130 131 , , , 132 133 a a DT 134 140 member member NN 141 143 of of IN 144 147 the the DT 148 155 phorbol phorbol NN 156 161 ester ester NN 162 175 nonresponsive nonresponsive JJ 176 184 atypical atypical JJ 185 192 protein protein NN 193 199 kinase kinase NNP 200 201 C C NNP 202 211 subfamily subfamily NN 211 212 , , , 213 215 in in IN 216 226 modulating modulate VBG 227 242 differentiation differentiation NN 243 246 was be VBD 247 255 examined examine VBN 256 258 in in IN 259 262 the the DT 263 271 leukemic leukemic JJ 272 276 U937 u937 NN 277 281 cell cell NN 281 282 . . . 283 294 Transfected transfecte VBN 295 299 U937 u937 NN 300 305 cells cell NNS 306 312 stably stably RB 313 327 overexpressing overexpresse VBG 328 336 PKC-zeta PKC-zeta NNP 337 346 displayed display VBD 347 348 a a DT 349 355 longer long JJR 356 364 doubling doubling NN 365 369 time time NN 369 370 , , , 371 376 lower low JJR 377 387 saturation saturation NN 388 395 density density NN 396 398 at at IN 399 409 confluency confluency NN 409 410 , , , 411 414 and and CC 415 417 an an DT 418 426 increase increase NN 427 429 in in IN 430 439 adherence adherence NN 440 442 to to TO 443 450 plastic plastic NN 451 453 as as IN 454 462 compared compare VBN 463 465 to to TO 466 473 control control NN 474 479 cells cell NNS 479 480 . . . 481 489 PKC-zeta PKC-zeta NNP 490 495 cells cell NNS 496 505 expressed express VBD 506 507 a a DT 508 512 more more RBR 513 527 differentiated differentiate VBN 528 537 phenotype phenotype NN 538 540 as as IN 541 549 assessed assess VBN 550 552 by by IN 553 560 changes change NNS 561 563 in in IN 564 574 morphology morphology NN 574 575 , , , 576 583 surface surface NN 584 591 antigen antigen NN 592 602 expression expression NN 602 603 , , , 604 607 and and CC 608 617 lysosomal lysosomal JJ 618 624 enzyme enzyme NN 625 635 activities activity NNS 636 639 and and CC 640 644 were be VBD 645 653 distinct distinct JJ 654 658 from from IN 659 667 parental parental JJ 668 672 U937 u937 NN 673 678 cells cell NNS 679 689 stimulated stimulate VBN 690 692 to to TO 693 706 differentiate differentiate VB 707 709 by by IN 710 718 exposure exposure NN 719 721 to to TO 722 729 phorbol phorbol NN 730 736 esters ester NNS 736 737 . . . 738 740 In in IN 741 749 contrast contrast NN 750 752 to to TO 753 761 parental parental JJ 762 766 U937 u937 NN 767 772 cells cell NNS 772 773 , , , 774 782 PKC-zeta PKC-zeta NNP 783 788 cells cell NNS 789 803 constitutively constitutively RB 804 813 expressed express VBD 814 818 mRNA mrna NN 819 830 transcripts transcript NNS 831 834 for for IN 835 840 c-jun c-jun NN 841 844 and and CC 845 846 a a DT 847 850 low low JJ 851 859 mobility mobility NN 860 864 AP-1 ap-1 NN 865 872 binding binding NN 873 881 activity activity NN 881 882 . . . 883 887 Thus thus RB 887 888 , , , 889 897 PKC-zeta PKC-zeta NNP 898 912 overexpression overexpression NN 913 923 stimulates stimulate VBZ 924 925 a a DT 926 930 type type NN 931 933 of of IN 934 944 phenotypic phenotypic JJ 945 960 differentiation differentiation NN 961 965 that that WDT 966 973 differs differ VBZ 974 987 significantly significantly RB 988 992 from from IN 993 1003 maturation maturation NN 1004 1013 occurring occur VBG 1014 1018 upon upon IN 1019 1029 activation activation NN 1030 1032 of of IN 1033 1038 other other JJ 1039 1042 PKC PKC NNP 1043 1054 subfamilies subfamily NNS 1055 1062 induced induce VBN 1063 1065 by by IN 1066 1073 phorbol phorbol NN 1074 1079 ester ester NN 1080 1089 treatment treatment NN 1089 1090 . . . 1091 1100 Increased increase VBN 1101 1111 expression expression NN 1112 1114 of of IN 1115 1118 the the DT 1119 1124 c-jun c-jun NN 1125 1138 protooncogene protooncogene NN 1139 1142 and and CC 1143 1145 an an DT 1146 1154 increase increase NN 1155 1157 in in IN 1158 1162 AP-1 ap-1 NN 1163 1170 binding binding NN 1171 1179 activity activity NN 1180 1182 in in IN 1183 1191 PKC-zeta PKC-zeta NNP 1192 1197 cells cell NNS 1198 1206 provides provide VBZ 1207 1208 a a DT 1209 1218 potential potential JJ 1219 1228 mechanism mechanism NN 1229 1232 for for IN 1233 1243 explaining explain VBG 1244 1247 the the DT 1248 1255 altered alter VBN 1256 1271 differentiation differentiation NN 1272 1278 status status NN 1279 1281 of of IN 1282 1286 this this DT 1287 1291 cell cell NN 1291 1292 . . .