0 9 Isolation isolation NN 10 12 of of IN 13 17 cDNA cdna NN 18 24 clones clone NNS 25 28 for for IN 29 31 42 42 CD 32 41 different different JJ 42 57 Kruppel-related kruppel-related JJ 58 62 zinc zinc NN 63 69 finger finger NN 70 78 proteins protein NNS 79 88 expressed express VBN 89 91 in in IN 92 95 the the DT 96 101 human human JJ 102 111 monoblast monoblast NN 112 116 cell cell NN 117 121 line line NN 122 127 U-937 u-937 NN 127 128 . . . 130 132 To to TO 133 138 study study VB 139 142 the the DT 143 153 complexity complexity NN 154 157 and and CC 158 168 structural structural JJ 169 184 characteristics characteristic NNS 185 187 of of IN 188 192 zinc zinc NN 193 199 finger finger NN 200 208 proteins protein NNS 209 218 expressed express VBN 219 225 during during IN 226 231 human human JJ 232 245 hematopoiesis hematopoiesis NN 246 249 and and CC 250 252 to to TO 253 260 isolate isolate VB 261 266 novel novel JJ 267 277 regulators regulator NNS 278 280 of of IN 281 286 blood blood NN 287 291 cell cell NN 292 303 development development NN 303 304 , , , 305 306 a a DT 307 317 degenerate degenerate JJ 318 333 oligonucleotide oligonucleotide NN 334 339 probe probe NN 340 348 specific specific JJ 349 352 for for IN 353 354 a a DT 355 364 consensus consensus NN 365 369 zinc zinc NN 370 376 finger finger NN 377 384 peptide peptide NN 385 391 domain domain NN 392 395 was be VBD 396 400 used use VBN 401 403 to to TO 404 411 isolate isolate VB 412 414 63 63 CD 415 419 cDNA cdna NN 420 426 clones clone NNS 427 430 for for IN 431 446 Kruppel-related kruppel-related JJ 447 451 zinc zinc NN 452 458 finger finger NN 459 464 genes gene NNS 465 469 from from IN 470 473 the the DT 474 479 human human JJ 480 489 monoblast monoblast NN 490 494 cell cell NN 495 499 line line NN 500 505 U-937 u-937 NN 505 506 . . . 507 509 By by IN 510 519 extensive extensive JJ 520 530 nucleotide nucleotide NN 531 539 sequence sequence NN 540 543 and and CC 544 552 Northern northern JJ 553 557 blot blot NN 558 566 analysis analysis NN 566 567 , , , 568 573 these these DT 574 578 cDNA cdna NN 579 585 clones clone NNS 586 590 were be VBD 591 596 found find VBN 597 599 to to TO 600 609 originate originate VB 610 614 from from IN 615 628 approximately approximately RB 629 631 42 42 CD 632 641 different different JJ 642 647 genes gene NNS 648 649 ( ( ( 649 652 HZF HZF NNP 653 657 1-42 1-42 CD 657 658 ) ) ) 659 661 of of IN 662 667 which which WDT 668 672 only only RB 673 674 8 8 CD 675 679 have have VBP 680 690 previously previously RB 691 695 been be VBN 696 705 described describe VBN 705 706 . . . 707 715 Northern Northern NNP 716 720 blot blot NN 721 729 analysis analysis NN 730 736 showed show VBD 737 741 that that IN 742 743 a a DT 744 752 majority majority NN 753 755 of of IN 756 761 these these DT 762 767 genes gene NNS 768 772 were be VBD 773 782 expressed express VBN 783 785 at at IN 786 796 comparable comparable JJ 797 803 levels level NNS 804 806 in in IN 807 812 U-937 U-937 NNP 813 816 and and CC 817 821 HeLa HeLa NNP 822 827 cells cell NNS 827 828 . . . 829 832 The the DT 833 838 large large JJ 839 845 number number NN 846 848 of of IN 849 859 individual individual JJ 860 865 genes gene NNS 866 877 represented represent VBN 878 883 among among IN 884 887 the the DT 888 890 63 63 CD 891 897 clones clone NNS 898 901 and and CC 902 907 their their PRP$ 908 916 apparent apparent JJ 917 939 non-cell-type-specific non-cell-type-specific JJ 940 950 expression expression NN 951 958 suggest suggest VBP 959 963 that that IN 964 967 the the DT 968 976 majority majority NN 977 979 of of IN 980 983 the the DT 984 999 Kruppel-related kruppel-related JJ 1000 1004 zinc zinc NN 1005 1011 finger finger NN 1012 1017 genes gene NNS 1018 1021 are be VBP 1022 1028 likely likely JJ 1029 1031 to to TO 1032 1034 be be VB 1035 1044 expressed express VBN 1045 1047 in in IN 1048 1052 most most JJS 1053 1058 human human JJ 1059 1066 tissues tissue NNS 1066 1067 . . . 1068 1070 In in IN 1071 1079 contrast contrast NN 1079 1080 , , , 1081 1085 some some DT 1086 1088 of of IN 1089 1092 the the DT 1093 1098 genes gene NNS 1099 1108 displayed display VBD 1109 1110 a a DT 1111 1121 restricted restricted JJ 1122 1132 expression expression NN 1133 1140 pattern pattern NN 1140 1141 , , , 1142 1152 indicating indicate VBG 1153 1157 that that IN 1158 1162 they they PRP 1163 1172 represent represent VBP 1173 1182 potential potential JJ 1183 1193 regulators regulator NNS 1194 1196 of of IN 1197 1205 monocyte monocyte NN 1206 1221 differentiation differentiation NN 1222 1224 or or CC 1225 1238 proliferation proliferation NN 1238 1239 . . . 1240 1248 Detailed detailed JJ 1249 1259 structural structural JJ 1260 1268 analysis analysis NN 1269 1271 of of IN 1272 1275 the the DT 1276 1281 first first JJ 1282 1284 12 12 CD 1285 1290 cDNAs cdna NNS 1291 1292 ( ( ( 1292 1295 HZF HZF NNP 1296 1300 1-10 1-10 CD 1300 1301 ) ) ) 1302 1305 and and CC 1306 1307 a a DT 1308 1315 partial partial JJ 1316 1332 characterization characterization NN 1333 1335 of of IN 1336 1339 HZF HZF NNP 1340 1345 11-42 11-42 CD 1346 1354 revealed reveal VBD 1355 1359 that that IN 1360 1361 a a DT 1362 1368 common common JJ 1369 1376 feature feature NN 1377 1379 of of IN 1380 1385 human human JJ 1386 1401 Kruppel-related kruppel-related JJ 1402 1406 zinc zinc NN 1407 1413 finger finger NN 1414 1422 proteins protein NNS 1423 1425 is be VBZ 1426 1429 the the DT 1430 1438 presence presence NN 1439 1441 of of IN 1442 1448 tandem tandem JJ 1449 1455 arrays array NNS 1456 1458 of of IN 1459 1463 zinc zinc NN 1464 1471 fingers finger NNS 1472 1479 ranging range VBG 1480 1482 in in IN 1483 1489 number number NN 1490 1494 from from IN 1495 1496 3 3 CD 1497 1499 to to TO 1500 1504 over over IN 1505 1507 20 20 CD 1508 1512 that that WDT 1513 1516 are be VBP 1517 1531 preferentially preferentially RB 1532 1539 located locate VBN 1540 1542 in in IN 1543 1546 the the DT 1547 1563 carboxy-terminal carboxy-terminal JJ 1564 1571 regions region NNS 1572 1574 of of IN 1575 1578 the the DT 1579 1587 proteins protein NNS 1587 1588 . . . 1589 1591 In in IN 1592 1600 addition addition NN 1600 1601 , , , 1602 1609 several several JJ 1610 1615 novel novel JJ 1616 1631 KRAB-containing krab-containing JJ 1632 1636 zinc zinc NN 1637 1643 finger finger NN 1644 1649 genes gene NNS 1650 1653 and and CC 1654 1655 a a DT 1656 1661 novel novel JJ 1662 1671 conserved conserve VBN 1672 1680 sequence sequence NN 1681 1688 element element NN 1689 1693 were be VBD 1694 1704 identified identify VBN 1704 1705 . . .