0 14 Identification identification NN 15 17 of of IN 18 23 human human JJ 24 27 TR2 tr2 NN 28 34 orphan orphan NN 35 43 receptor receptor NN 44 52 response response NN 53 60 element element NN 61 63 in in IN 64 67 the the DT 68 83 transcriptional transcriptional JJ 84 94 initiation initiation NN 95 99 site site NN 100 102 of of IN 103 106 the the DT 107 113 simian simian JJ 114 119 virus virus NN 120 122 40 40 CD 123 128 major major JJ 129 133 late late JJ 134 142 promoter promoter NN 143 144 [ [ ( 144 153 published publish VBN 154 161 erratum erratum NN 162 169 appears appear VBZ 170 172 in in IN 173 174 J J NNP 175 179 Biol Biol NNP 180 184 Chem Chem NNP 185 189 1995 1995 CD 190 193 Nov nov IN 194 209 3;270(44):26721 3;270(44):26721 CD 209 210 ] ] ) 212 213 A a NN 214 217 DNA DNA NNP 218 226 response response NN 227 234 element element NN 235 236 ( ( ( 236 246 TR2RE-SV40 TR2RE-SV40 NNP 246 247 ) ) ) 248 251 for for IN 252 255 the the DT 256 259 TR2 tr2 NN 260 266 orphan orphan NN 267 275 receptor receptor NN 275 276 , , , 277 278 a a DT 279 285 member member NN 286 288 of of IN 289 292 the the DT 293 308 steroid-thyroid steroid-thyroid JJ 309 316 hormone hormone NN 317 325 receptor receptor NN 326 337 superfamily superfamily NN 337 338 , , , 339 342 has have VBZ 343 347 been be VBN 348 358 identified identify VBN 359 361 in in IN 362 365 the the DT 366 372 simian simian JJ 373 378 virus virus NN 379 381 40 40 CD 382 383 ( ( ( 383 387 SV40 sv40 NN 387 388 ) ) ) 389 392 +55 +55 CD 393 399 region region NN 400 401 ( ( ( 401 411 nucleotide nucleotide NN 412 419 numbers number NNS 420 427 368-389 368-389 CD 427 428 , , , 429 456 5’-GTTAAGGTTCGTAGGTCATGGA-3 5'-gttaaggttcgtaggtcatgga-3 NN 456 457 ’ ' '' 457 458 ) ) ) 458 459 . . . 460 475 Electrophoretic electrophoretic JJ 476 484 mobility mobility NN 485 490 shift shift NN 491 496 assay assay NN 496 497 , , , 498 503 using use VBG 504 506 in in FW 507 512 vitro vitro FW 513 523 translated translate VBN 524 527 TR2 tr2 NN 528 534 orphan orphan NN 535 543 receptor receptor NN 544 548 with with IN 549 550 a a DT 551 560 molecular molecular JJ 561 565 mass mass NN 566 568 of of IN 569 571 67 67 CD 572 583 kilodaltons kilodalton NNS 583 584 , , , 585 591 showed show VBD 592 593 a a DT 594 602 specific specific JJ 603 610 binding binding NN 611 615 with with IN 616 620 high high JJ 621 629 affinity affinity NN 630 631 ( ( ( 631 643 dissociation dissociation NN 644 652 constant constant NN 653 654 = = SYM 655 656 9 9 CD 657 659 nM nm NN 659 660 ) ) ) 661 664 for for IN 665 669 this this DT 670 673 DNA dna NN 674 682 sequence sequence NN 682 683 . . . 684 692 DNA-swap dna-swap JJ 693 704 experiments experiment NNS 705 710 using use VBG 711 726 chloramphenicol chloramphenicol NN 727 745 acetyl-transferase acetyl-transferase NN 746 751 assay assay NN 752 764 demonstrated demonstrate VBD 765 769 that that IN 770 778 androgen androgen NN 779 782 can can MD 783 791 suppress suppress VB 792 795 the the DT 796 811 transcriptional transcriptional JJ 812 822 activities activity NNS 823 825 of of IN 826 830 SV40 SV40 NNP 831 836 early early JJ 837 845 promoter promoter NN 846 849 via via IN 850 853 the the DT 854 865 interaction interaction NN 866 873 between between IN 874 878 this this DT 879 889 TR2RE-SV40 tr2re-sv40 NN 890 893 and and CC 894 897 the the DT 898 906 chimeric chimeric JJ 907 915 receptor receptor NN 916 925 AR/TR2/AR ar/tr2/ar NN 926 930 with with IN 931 934 the the DT 935 946 DNA-binding dna-binding JJ 947 953 domain domain NN 954 956 of of IN 957 960 the the DT 961 964 TR2 tr2 NN 965 971 orphan orphan NN 972 980 receptor receptor NN 981 988 flanked flank VBN 989 991 by by IN 992 995 the the DT 996 1006 N-terminal n-terminal JJ 1007 1010 and and CC 1011 1027 androgen-binding androgen-binding JJ 1028 1035 domains domain NNS 1036 1038 of of IN 1039 1042 the the DT 1043 1051 androgen androgen NN 1052 1060 receptor receptor NN 1060 1061 . . . 1062 1064 In in IN 1065 1073 addition addition NN 1073 1074 , , , 1075 1079 this this DT 1080 1090 TR2RE-SV40 tr2re-sv40 NN 1091 1094 can can MD 1095 1103 function function VB 1104 1106 as as IN 1107 1108 a a DT 1109 1118 repressor repressor NN 1119 1121 to to TO 1122 1130 suppress suppress VB 1131 1134 the the DT 1135 1150 transcriptional transcriptional JJ 1151 1161 activities activity NNS 1162 1164 of of IN 1165 1169 both both CC 1170 1174 SV40 sv40 NN 1175 1180 early early JJ 1181 1184 and and CC 1185 1189 late late JJ 1190 1199 promoters promoter NNS 1199 1200 . . . 1201 1209 Together together RB 1209 1210 , , , 1211 1216 these these DT 1217 1221 data datum NNS 1222 1229 suggest suggest VBP 1230 1233 the the DT 1234 1244 TR2RE-SV40 tr2re-sv40 NN 1245 1248 may may MD 1249 1258 represent represent VB 1259 1262 the the DT 1263 1268 first first JJ 1269 1279 identified identify VBN 1280 1287 natural natural JJ 1288 1291 DNA dna NN 1292 1300 response response NN 1301 1308 element element NN 1309 1312 for for IN 1313 1316 the the DT 1317 1320 TR2 tr2 NN 1321 1327 orphan orphan NN 1328 1336 receptor receptor NN 1337 1341 that that WDT 1342 1345 may may MD 1346 1354 function function VB 1355 1357 as as IN 1358 1359 a a DT 1360 1369 repressor repressor NN 1370 1373 for for IN 1374 1377 the the DT 1378 1382 SV40 SV40 NNP 1383 1387 gene gene NN 1388 1398 expression expression NN 1398 1399 . . .