0 1 [ [ ( 1 11 Regulation regulation NN 12 14 of of IN 15 28 transcription transcription NN 29 31 of of IN 32 35 the the DT 36 49 interleukin-2 interleukin-2 NN 50 54 gene gene NN 55 57 in in IN 58 71 B-lymphocytes B-lymphocyte NNP 71 72 ] ] ) 74 79 Since since IN 80 84 most most JJS 85 86 B B NNP 87 91 cell cell NN 92 98 clones clone NNS 99 111 immortalized immortalize VBN 112 116 with with IN 117 120 EBV EBV NNP 121 126 virus virus NN 127 130 can can MD 131 133 be be VB 134 141 induced induce VBN 142 144 to to TO 145 152 produce produce VB 153 166 interleukin-2 interleukin-2 NN 166 167 , , , 168 169 a a DT 170 177 typical typical JJ 178 179 T t NN 180 184 cell cell NN 185 193 cytokine cytokine NN 193 194 , , , 195 197 we we PRP 198 205 studied study VBD 206 209 the the DT 210 214 role role NN 215 217 of of IN 218 227 different different JJ 228 236 elements element NNS 237 239 of of IN 240 243 the the DT 244 248 IL-2 il-2 NN 249 257 promoter promoter NN 258 260 in in IN 261 265 such such JJ 266 272 clones clone NNS 273 275 by by IN 276 288 transfection transfection NN 288 289 . . . 290 292 It it PRP 293 296 was be VBD 297 302 found find VBN 302 303 , , , 304 306 in in IN 307 317 particular particular JJ 317 318 , , , 319 323 that that IN 324 327 the the DT 328 335 element element NN 336 340 TCEd TCEd NNP 340 341 , , , 342 347 which which WDT 348 353 binds bind VBZ 354 357 the the DT 358 371 transcription transcription NN 372 378 factor factor NN 379 384 NF-kB NF-kB NNP 384 385 , , , 386 388 is be VBZ 389 393 very very RB 394 400 active active JJ 401 403 in in IN 404 407 all all DT 408 413 three three CD 414 415 B B NNP 416 422 clones clone NNS 423 429 tested test VBN 429 430 . . . 431 435 This this DT 436 443 element element NN 444 447 has have VBZ 448 450 no no DT 451 459 activity activity NN 460 462 in in IN 463 464 T t NN 465 470 cells cell NNS 471 473 of of IN 474 477 the the DT 478 484 Jurkat Jurkat NNP 485 489 line line NN 489 490 . . . 491 494 The the DT 495 500 NFATd nfatd NN 501 508 element element NN 508 509 , , , 510 515 which which WDT 516 521 binds bind VBZ 522 525 the the DT 526 539 transcription transcription NN 540 546 factor factor NN 547 553 NFAT-1 nfat-1 NN 554 557 and and CC 558 560 is be VBZ 561 565 very very RB 566 572 active active JJ 573 575 in in IN 576 577 T t NN 578 583 cells cell NNS 583 584 , , , 585 587 is be VBZ 588 592 only only RB 593 599 weakly weakly RB 600 606 active active JJ 607 609 in in IN 610 613 one one CD 614 615 B B NNP 616 621 clone clone NN 622 625 and and CC 626 629 not not RB 630 632 at at IN 633 636 all all DT 637 639 in in IN 640 647 another another DT 647 648 . . . 649 658 Different different JJ 659 667 elements element NNS 668 672 thus thus RB 673 683 contribute contribute VBP 684 686 to to TO 687 691 IL-2 il-2 NN 692 700 promoter promoter NN 701 709 activity activity NN 710 712 in in IN 713 722 different different JJ 723 728 cells cell NNS 728 729 . . .