0 3 ERP ERP NNP 3 4 , , , 5 6 a a DT 7 10 new new JJ 11 17 member member NN 18 20 of of IN 21 24 the the DT 25 28 ets ets NN 29 42 transcription transcription NN 43 61 factor/oncoprotein factor/oncoprotein NN 62 68 family family NN 68 69 : : : 70 77 cloning cloning NN 77 78 , , , 79 95 characterization characterization NN 95 96 , , , 97 100 and and CC 101 113 differential differential JJ 114 124 expression expression NN 125 131 during during IN 132 144 B-lymphocyte B-lymphocyte NNP 145 156 development development NN 156 157 . . . 159 162 The the DT 163 166 ets ets NN 167 171 gene gene NN 172 178 family family NN 179 186 encodes encode VBZ 187 188 a a DT 189 194 group group NN 195 197 of of IN 198 206 proteins protein NNS 207 212 which which WDT 213 221 function function VBP 222 224 as as IN 225 238 transcription transcription NN 239 246 factors factor NNS 247 252 under under IN 253 266 physiological physiological JJ 267 277 conditions condition NNS 278 281 and and CC 281 282 , , , 283 285 if if IN 286 296 aberrantly aberrantly RB 297 306 expressed express VBN 306 307 , , , 308 311 can can MD 312 317 cause cause VB 318 326 cellular cellular JJ 327 341 transformation transformation NN 341 342 . . . 343 345 We we PRP 346 350 have have VBP 351 359 recently recently RB 360 370 identified identify VBN 371 374 two two CD 375 385 regulatory regulatory JJ 386 394 elements element NNS 395 397 in in IN 398 401 the the DT 402 408 murine murine JJ 409 423 immunoglobulin immunoglobulin NN 424 435 heavy-chain heavy-chain NN 436 437 ( ( ( 437 440 IgH IgH NNP 440 441 ) ) ) 442 450 enhancer enhancer NN 450 451 , , , 452 454 pi pi NN 455 458 and and CC 459 465 microB microb NN 465 466 , , , 467 472 which which WDT 473 480 exhibit exhibit VBP 481 489 striking striking JJ 490 500 similarity similarity NN 501 503 to to TO 504 511 binding bind VBG 512 517 sites site NNS 518 521 for for IN 522 533 ets-related ets-related JJ 534 542 proteins protein NNS 542 543 . . . 544 546 To to TO 547 555 identify identify VB 556 567 ets-related ets-related JJ 568 583 transcriptional transcriptional JJ 584 594 regulators regulator NNS 595 604 expressed express VBN 605 607 in in IN 608 613 pre-B pre-b JJ 614 625 lymphocytes lymphocyte NNS 626 630 that that WDT 631 634 may may MD 635 643 interact interact VB 644 648 with with IN 649 655 either either CC 656 659 the the DT 660 662 pi pi NN 663 665 or or CC 666 669 the the DT 670 676 microB microb NN 677 681 site site NN 681 682 , , , 683 685 we we PRP 686 690 have have VBP 691 695 used use VBN 696 697 a a DT 698 701 PCR PCR NNP 702 710 approach approach NN 711 715 with with IN 716 726 degenerate degenerate JJ 727 743 oligonucleotides oligonucleotide NNS 744 752 encoding encode VBG 753 762 conserved conserve VBN 763 772 sequences sequence NNS 773 775 in in IN 776 779 all all DT 780 787 members member NNS 788 790 of of IN 791 794 the the DT 795 798 ets ets NN 799 805 family family NN 805 806 . . . 807 809 We we PRP 810 814 have have VBP 815 821 cloned clone VBN 822 825 the the DT 826 830 gene gene NN 831 834 for for IN 835 836 a a DT 837 840 new new JJ 841 852 ets-related ets-related JJ 853 866 transcription transcription NN 867 873 factor factor NN 873 874 , , , 875 878 ERP ERP NNP 879 880 ( ( ( 880 891 ets-related ets-related JJ 892 899 protein protein NN 899 900 ) ) ) 900 901 , , , 902 906 from from IN 907 910 the the DT 911 917 murine murine JJ 918 923 pre-B pre-b JJ 924 928 cell cell NN 929 933 line line NN 934 938 BASC BASC NNP 939 942 6C2 6c2 NN 943 946 and and CC 947 951 from from IN 952 957 mouse mouse NN 958 962 lung lung NN 963 969 tissue tissue NN 969 970 . . . 971 974 The the DT 975 978 ERP ERP NNP 979 986 protein protein NN 987 995 contains contain VBZ 996 997 a a DT 998 1004 region region NN 1005 1007 of of IN 1008 1012 high high JJ 1013 1021 homology homology NN 1022 1026 with with IN 1027 1030 the the DT 1031 1034 ETS ets NN 1035 1046 DNA-binding dna-binding JJ 1047 1053 domain domain NN 1054 1060 common common JJ 1061 1063 to to TO 1064 1067 all all DT 1068 1075 members member NNS 1076 1078 of of IN 1079 1082 the the DT 1083 1086 ets ets NN 1087 1100 transcription transcription NN 1101 1119 factor/oncoprotein factor/oncoprotein NN 1120 1126 family family NN 1126 1127 . . . 1128 1133 Three three CD 1134 1144 additional additional JJ 1145 1152 smaller small JJR 1153 1160 regions region NNS 1161 1165 show show VBP 1166 1174 homology homology NN 1175 1177 to to TO 1178 1181 the the DT 1182 1187 ELK-1 elk-1 NN 1188 1191 and and CC 1192 1197 SAP-1 sap-1 NN 1198 1203 genes gene NNS 1203 1204 , , , 1205 1206 a a DT 1207 1215 subgroup subgroup NN 1216 1218 of of IN 1219 1222 the the DT 1223 1226 ets ets NN 1227 1231 gene gene NN 1232 1238 family family NN 1239 1243 that that WDT 1244 1253 interacts interact VBZ 1254 1258 with with IN 1259 1262 the the DT 1263 1268 serum serum NN 1269 1277 response response NN 1278 1284 factor factor NN 1284 1285 . . . 1286 1297 Full-length full-length JJ 1298 1301 ERP ERP NNP 1302 1311 expresses express VBZ 1312 1316 only only RB 1317 1327 negligible negligible JJ 1328 1339 DNA-binding dna-binding JJ 1340 1348 activity activity NN 1349 1351 by by IN 1352 1358 itself itself PRP 1358 1359 . . . 1360 1367 Removal Removal NNP 1368 1370 of of IN 1371 1374 the the DT 1375 1382 carboxy carboxy NN 1383 1391 terminus terminus NN 1392 1399 enables enable VBZ 1400 1403 ERP ERP NNP 1404 1406 to to TO 1407 1415 interact interact VB 1416 1420 with with IN 1421 1422 a a DT 1423 1430 variety variety NN 1431 1433 of of IN 1434 1445 ets-binding ets-binding JJ 1446 1451 sites site NNS 1452 1461 including include VBG 1462 1465 the the DT 1466 1469 E74 e74 NN 1470 1474 site site NN 1474 1475 , , , 1476 1479 the the DT 1480 1483 IgH IgH NNP 1484 1492 enhancer enhancer NN 1493 1495 pi pi NN 1496 1500 site site NN 1500 1501 , , , 1502 1505 and and CC 1506 1509 the the DT 1510 1513 lck lck NN 1514 1522 promoter promoter NN 1523 1526 ets ets NN 1527 1531 site site NN 1531 1532 , , , 1533 1543 suggesting suggest VBG 1544 1545 a a DT 1546 1562 carboxy-terminal carboxy-terminal JJ 1563 1571 negative negative JJ 1572 1582 regulatory regulatory JJ 1583 1589 domain domain NN 1589 1590 . . . 1591 1593 At at IN 1594 1599 least least JJS 1600 1605 three three CD 1606 1617 ERP-related erp-related JJ 1618 1629 transcripts transcript NNS 1630 1633 are be VBP 1634 1643 expressed express VBN 1644 1646 in in IN 1647 1648 a a DT 1649 1656 variety variety NN 1657 1659 of of IN 1660 1667 tissues tissue NNS 1667 1668 . . . 1669 1676 However however RB 1676 1677 , , , 1678 1684 within within IN 1685 1688 the the DT 1689 1695 B-cell b-cell NN 1696 1703 lineage lineage NN 1703 1704 , , , 1705 1708 ERP ERP NNP 1709 1711 is be VBZ 1712 1718 highly highly RB 1719 1728 expressed express VBN 1729 1738 primarily primarily RB 1739 1741 at at IN 1742 1747 early early JJ 1748 1754 stages stage NNS 1755 1757 of of IN 1758 1770 B-lymphocyte B-lymphocyte NNP 1771 1782 development development NN 1782 1783 , , , 1784 1787 and and CC 1788 1798 expression expression NN 1799 1807 declines decline VBZ 1808 1819 drastically drastically RB 1820 1824 upon upon IN 1825 1831 B-cell b-cell NN 1832 1842 maturation maturation NN 1842 1843 , , , 1844 1855 correlating correlate VBG 1856 1860 with with IN 1861 1864 the the DT 1865 1873 enhancer enhancer NN 1874 1882 activity activity NN 1883 1885 of of IN 1886 1889 the the DT 1890 1893 IgH IgH NNP 1894 1896 pi pi NN 1897 1901 site site NN 1901 1902 . . . 1903 1908 These these DT 1909 1913 data datum NNS 1914 1921 suggest suggest VBP 1922 1926 that that IN 1927 1930 ERP ERP NNP 1931 1936 might might MD 1937 1941 play play VB 1942 1943 a a DT 1944 1948 role role NN 1949 1951 in in IN 1952 1958 B-cell b-cell NN 1959 1970 development development NN 1971 1974 and and CC 1975 1977 in in IN 1978 1981 IgH IgH NNP 1982 1986 gene gene NN 1987 1997 regulation regulation NN 1997 1998 . . .