0 1 T t NN 2 7 cells cell NNS 8 12 from from IN 13 18 renal renal JJ 19 23 cell cell NN 24 33 carcinoma carcinoma NN 34 42 patients patient NNS 43 50 exhibit exhibit VBP 51 53 an an DT 54 62 abnormal abnormal JJ 63 70 pattern pattern NN 71 73 of of IN 74 79 kappa kappa NN 80 90 B-specific b-specific JJ 91 102 DNA-binding dna-binding JJ 103 111 activity activity NN 111 112 : : : 113 114 a a DT 115 126 preliminary preliminary JJ 127 133 report report NN 133 134 . . . 136 142 Recent recent JJ 143 147 data datum NNS 148 155 suggest suggest VBP 156 160 that that IN 161 164 the the DT 165 169 poor poor JJ 170 179 induction induction NN 180 182 of of IN 183 184 a a DT 185 191 T-cell t-cell NN 192 200 response response NN 201 203 to to TO 204 209 human human JJ 210 215 renal renal JJ 216 220 cell cell NN 221 230 carcinoma carcinoma NN 231 232 ( ( ( 232 235 RCC RCC NNP 235 236 ) ) ) 237 240 may may MD 241 243 be be VB 244 251 related relate VBN 252 254 to to TO 255 266 alterations alteration NNS 267 269 in in IN 270 276 signal signal NN 277 289 transduction transduction NN 290 298 pathways pathway NNS 298 299 . . . 300 302 We we PRP 303 309 report report VBP 310 314 that that IN 315 316 T t NN 317 322 cells cell NNS 323 327 from from IN 328 331 RCC RCC NNP 332 340 patients patient NNS 341 345 have have VBP 346 349 two two CD 350 361 alterations alteration NNS 362 364 in in IN 365 370 kappa kappa NN 371 372 B B NNP 373 387 motif-specific motif-specific JJ 388 399 DNA-binding dna-binding JJ 400 408 activity activity NN 408 409 . . . 410 413 The the DT 414 419 first first JJ 420 430 alteration alteration NN 431 439 involves involve VBZ 440 443 the the DT 444 456 constitutive constitutive JJ 457 467 expression expression NN 468 470 of of IN 471 482 substantial substantial JJ 483 488 kappa kappa NN 489 498 B-binding b-binding JJ 499 507 activity activity NN 508 510 in in IN 511 518 nuclear nuclear JJ 519 527 extracts extract NNS 527 528 , , , 529 534 which which WDT 535 538 was be VBD 539 547 observed observe VBN 548 550 in in IN 551 554 the the DT 555 570 electrophoretic electrophoretic JJ 571 579 mobility mobility NN 580 585 shift shift NN 586 591 assay assay NN 591 592 . . . 593 596 The the DT 597 606 magnitude magnitude NN 607 609 of of IN 610 615 kappa kappa NN 616 617 B B NNP 618 626 activity activity NN 627 629 in in IN 630 642 unstimulated unstimulated JJ 643 650 patient patient NN 651 652 T t NN 653 658 cells cell NNS 659 662 was be VBD 663 670 similar similar JJ 671 673 to to TO 674 678 that that DT 679 687 observed observe VBN 688 690 in in IN 691 692 T t NN 693 698 cells cell NNS 699 703 from from IN 704 710 normal normal JJ 711 722 individuals individual NNS 723 727 that that WDT 728 731 had have VBD 732 736 been be VBN 737 746 activated activate VBN 747 749 in in FW 750 755 vitro vitro FW 755 756 . . . 757 759 On on IN 760 763 the the DT 764 769 basis basis NN 770 772 of of IN 773 780 Western western JJ 781 789 blotting blotting NN 790 801 experiments experiment NNS 802 807 using use VBG 808 818 antibodies antibody NNS 819 821 to to TO 822 827 kappa kappa NN 828 833 B/Rel B/Rel NNP 834 840 family family NN 841 849 proteins protein NNS 849 850 , , , 851 854 the the DT 855 860 kappa kappa NN 861 870 B-binding b-binding JJ 871 879 activity activity NN 880 894 constitutively constitutively RB 895 904 expressed express VBN 905 907 in in IN 908 909 T t NN 910 915 cells cell NNS 916 920 from from IN 921 924 RCC RCC NNP 925 933 patients patient NNS 934 936 is be VBZ 937 945 composed compose VBN 946 952 mostly mostly RB 953 955 of of IN 956 959 the the DT 960 968 NF-kappa NF-kappa NNP 969 971 B1 B1 NNP 972 973 ( ( ( 973 976 p50 p50 NN 976 977 ) ) ) 978 985 subunit subunit NN 985 986 . . . 987 990 The the DT 991 997 second second JJ 998 1009 abnormality abnormality NN 1010 1012 in in IN 1013 1018 kappa kappa NN 1019 1028 B-binding b-binding JJ 1029 1037 activity activity NN 1038 1040 in in IN 1041 1042 T t NN 1043 1048 cells cell NNS 1049 1053 from from IN 1054 1059 these these DT 1060 1068 patients patient NNS 1069 1071 is be VBZ 1072 1076 that that IN 1077 1081 RelA RelA NNP 1081 1082 , , , 1083 1084 a a DT 1085 1091 member member NN 1092 1094 of of IN 1095 1098 the the DT 1099 1102 Rel Rel NNP 1103 1111 homology homology NN 1112 1118 family family NN 1119 1124 which which WDT 1125 1127 is be VBZ 1128 1132 part part NN 1133 1135 of of IN 1136 1139 the the DT 1140 1146 normal normal JJ 1147 1155 NF-kappa NF-kappa NNP 1156 1157 B B NNP 1158 1165 complex complex NN 1165 1166 , , , 1167 1170 was be VBD 1171 1174 not not RB 1175 1182 induced induce VBN 1183 1185 in in IN 1186 1189 the the DT 1190 1197 nucleus nucleus NN 1198 1207 following follow VBG 1208 1218 activation activation NN 1218 1219 . . . 1220 1227 Western western NN 1228 1236 blotting blotting NN 1237 1245 analysis analysis NN 1246 1249 did do VBD 1250 1253 not not RB 1254 1260 detect detect VB 1261 1264 any any DT 1265 1269 RelA rela NN 1270 1272 in in IN 1273 1280 nuclear nuclear JJ 1281 1289 extracts extract NNS 1290 1296 either either CC 1297 1303 before before IN 1304 1306 or or CC 1307 1312 after after IN 1313 1324 stimulation stimulation NN 1325 1327 of of IN 1328 1329 T t NN 1330 1335 cells cell NNS 1335 1336 . . . 1337 1340 The the DT 1341 1348 altered altered JJ 1349 1354 kappa kappa NN 1355 1364 B-binding b-binding JJ 1365 1373 activity activity NN 1374 1376 in in IN 1377 1378 T t NN 1379 1384 cells cell NNS 1385 1389 from from IN 1390 1393 RCC RCC NNP 1394 1402 patients patient NNS 1403 1406 may may MD 1407 1413 impair impair VB 1414 1419 their their PRP$ 1420 1428 capacity capacity NN 1429 1431 to to TO 1432 1439 respond respond VB 1440 1448 normally normally RB 1449 1451 to to TO 1452 1459 various various JJ 1460 1467 stimuli stimulus NNS 1467 1468 . . .