0 1 A a DT 2 8 family family NN 9 11 of of IN 12 18 serine serine NN 19 28 proteases protease NNS 29 38 expressed express VBN 39 50 exclusively exclusively RB 51 53 in in IN 54 69 myelo-monocytic myelo-monocytic JJ 70 75 cells cell NNS 76 88 specifically specifically RB 89 98 processes process VBZ 99 102 the the DT 103 110 nuclear nuclear JJ 111 123 factor-kappa factor-kappa NN 124 125 B B NNP 126 133 subunit subunit NN 134 137 p65 p65 NN 138 140 in in FW 141 146 vitro vitro FW 147 150 and and CC 151 154 may may MD 155 161 impair impair VB 162 167 human human JJ 168 184 immunodeficiency immunodeficiency NN 185 190 virus virus NN 191 202 replication replication NN 203 205 in in IN 206 211 these these DT 212 217 cells cell NNS 217 218 . . . 220 223 Two two CD 224 230 groups group NNS 231 233 of of IN 234 238 U937 u937 NN 239 251 promonocytic promonocytic JJ 252 257 cells cell NNS 258 262 were be VBD 263 271 obtained obtain VBN 272 274 by by IN 275 283 limiting limit VBG 284 292 dilution dilution NN 293 300 cloning cloning NN 301 306 which which WDT 307 315 differed differ VBD 316 326 strikingly strikingly RB 327 329 in in IN 330 335 their their PRP$ 336 343 ability ability NN 344 346 to to TO 347 354 support support VB 355 360 human human JJ 361 377 immunodeficiency immunodeficiency NN 378 383 virus virus NN 384 385 1 1 CD 386 387 ( ( ( 387 392 HIV-1 HIV-1 NNP 392 393 ) ) ) 394 405 replication replication NN 405 406 . . . 407 408 “ `` `` 408 412 Plus plus JJ 412 413 ” '' '' 414 420 clones clone NNS 421 431 replicated replicate VBD 432 435 the the DT 436 441 virus virus NN 442 453 efficiently efficiently RB 453 454 , , , 455 462 whereas whereas IN 463 464 “ `` `` 464 469 minus minus JJ 469 470 ” '' '' 471 477 clones clone NNS 478 481 did do VBD 482 485 not not RB 485 486 . . . 487 489 We we PRP 490 498 examined examine VBD 499 504 these these DT 505 511 clones clone NNS 512 515 for for IN 516 527 differences difference NNS 528 530 in in IN 531 538 nuclear nuclear JJ 539 545 factor factor NN 546 547 ( ( ( 547 556 NF)-kappa NF)-kappa NNP 557 558 B B NNP 559 567 activity activity NN 568 573 which which WDT 574 579 might might MD 580 587 account account VB 588 591 for for IN 592 595 the the DT 596 604 observed observe VBN 605 615 phenomenon phenomenon NN 615 616 . . . 617 628 Stimulation stimulation NN 629 631 of of IN 632 636 plus plus CC 637 643 clones clone NNS 644 653 liberated liberate VBD 654 657 the the DT 658 667 classical classical JJ 668 675 p50-p65 p50-p65 NN 676 683 complex complex NN 684 688 from from IN 689 700 cytoplasmic cytoplasmic JJ 701 706 pools pool NNS 706 707 , , , 708 715 whereas whereas IN 716 721 minus minus JJ 722 728 clones clone NNS 729 737 produced produce VBD 738 740 an an DT 741 751 apparently apparently RB 752 757 novel novel JJ 757 758 , , , 759 775 faster-migrating faster-migrating JJ 776 783 complex complex NN 783 784 , , , 785 787 as as IN 788 794 judged judge VBN 795 797 by by IN 798 813 electrophoretic electrophoretic JJ 814 822 mobility mobility NN 823 828 shift shift NN 829 835 assays assay NNS 835 836 . . . 837 839 It it PRP 840 842 is be VBZ 843 853 surprising surprising JJ 854 858 that that IN 859 862 the the DT 863 879 faster-migrating faster-migrating JJ 880 887 complex complex NN 888 891 was be VBD 892 900 composed compose VBN 901 905 also also RB 906 908 of of IN 909 912 p50 p50 NN 913 916 and and CC 917 920 p65 p65 NN 920 921 . . . 922 929 However however RB 929 930 , , , 931 934 the the DT 935 938 p65 p65 NN 939 946 subunit subunit NN 947 950 was be VBD 951 966 COOH-terminally cooh-terminally RB 967 976 truncated truncate VBN 976 977 , , , 978 980 as as IN 981 986 shown show VBN 987 989 by by IN 990 1009 immunoprecipitation immunoprecipitation NN 1009 1010 . . . 1011 1014 The the DT 1015 1025 truncation truncation NN 1026 1034 resulted result VBD 1035 1039 from from IN 1040 1047 limited limited JJ 1048 1059 proteolysis proteolysis NN 1060 1062 of of IN 1063 1066 p65 p65 NN 1067 1073 during during IN 1074 1082 cellular cellular JJ 1083 1093 extraction extraction NN 1094 1099 which which WDT 1100 1108 released release VBD 1109 1119 particular particular JJ 1120 1129 lysosomal lysosomal JJ 1130 1136 serine serine NN 1137 1146 proteases protease NNS 1146 1147 , , , 1148 1152 such such JJ 1153 1155 as as IN 1156 1164 elastase elastase NN 1164 1165 , , , 1166 1175 cathepsin cathepsin NN 1176 1177 G G NNP 1177 1178 , , , 1179 1182 and and CC 1183 1193 proteinase proteinase NNP 1194 1195 3 3 CD 1195 1196 . . . 1197 1202 These these DT 1203 1211 specific specific JJ 1212 1221 proteases protease NNS 1222 1225 are be VBP 1226 1238 coordinately coordinately RB 1239 1248 expressed express VBN 1249 1252 and and CC 1253 1257 were be VBD 1258 1265 present present JJ 1266 1277 exclusively exclusively RB 1278 1280 in in IN 1281 1284 the the DT 1285 1290 minus minus JJ 1291 1295 U937 u937 NN 1296 1302 clones clone NNS 1302 1303 , , , 1304 1307 but but CC 1308 1311 not not RB 1312 1314 in in IN 1315 1318 the the DT 1319 1323 plus plus JJ 1324 1330 clones clone NNS 1330 1331 , , , 1332 1334 as as IN 1335 1347 demonstrated demonstrate VBN 1348 1350 in in IN 1351 1354 the the DT 1355 1359 case case NN 1360 1362 of of IN 1363 1372 cathepsin cathepsin NN 1373 1374 G G NNP 1374 1375 . . . 1376 1378 In in IN 1379 1387 addition addition NN 1387 1388 , , , 1389 1394 these these DT 1395 1404 proteases protease NNS 1405 1409 were be VBD 1410 1418 detected detect VBN 1419 1421 in in IN 1422 1429 certain certain JJ 1430 1439 subclones subclone NNS 1440 1442 of of IN 1443 1448 THP-1 thp-1 NN 1449 1452 and and CC 1453 1458 HL-60 hl-60 NN 1459 1464 cells cell NNS 1465 1468 and and CC 1469 1471 in in IN 1472 1479 primary primary JJ 1480 1489 monocytes monocyte NNS 1489 1490 , , , 1491 1493 in in IN 1494 1498 each each DT 1499 1503 case case NN 1504 1515 correlating correlate VBG 1516 1520 with with IN 1521 1524 the the DT 1525 1534 truncated truncate VBN 1535 1539 from from IN 1540 1542 of of IN 1543 1546 p65 p65 NN 1546 1547 . . . 1548 1550 We we PRP 1551 1562 demonstrate demonstrate VBP 1563 1565 in in FW 1566 1571 vitro vitro FW 1572 1580 cleavage cleavage NN 1581 1583 of of IN 1584 1587 p65 p65 NN 1588 1590 by by IN 1591 1599 purified purify VBN 1600 1608 elastase elastase NN 1609 1612 and and CC 1613 1622 cathepsin cathepsin NN 1623 1624 G G NNP 1624 1625 . . . 1626 1628 It it PRP 1629 1631 is be VBZ 1632 1640 possible possible JJ 1641 1645 that that IN 1646 1656 particular particular JJ 1657 1663 serine serine NN 1664 1673 proteases protease NNS 1674 1677 may may MD 1678 1682 have have VB 1683 1693 inhibiting inhibit VBG 1694 1701 effects effect NNS 1702 1704 on on IN 1705 1708 the the DT 1709 1720 replication replication NN 1721 1723 of of IN 1724 1729 HIV-1 HIV-1 NNP 1730 1732 in in IN 1733 1748 myelo-monocytic myelo-monocytic JJ 1749 1754 cells cell NNS 1754 1755 . . . 1756 1759 The the DT 1760 1764 data datum NNS 1765 1769 also also RB 1770 1781 demonstrate demonstrate VBP 1782 1786 that that IN 1787 1794 special special JJ 1795 1806 precautions precaution NNS 1807 1811 must must MD 1812 1814 be be VB 1815 1820 taken take VBN 1821 1825 when when WRB 1826 1832 making make VBG 1833 1841 extracts extract NNS 1842 1846 from from IN 1847 1862 myelo-monocytic myelo-monocytic JJ 1863 1868 cells cell NNS 1868 1869 . . .