0 9 Increased increase VBN 10 21 interleukin interleukin NN 22 23 2 2 CD 24 37 transcription transcription NN 38 40 in in IN 41 47 murine murine JJ 48 59 lymphocytes lymphocyte NNS 60 62 by by IN 63 76 ciprofloxacin ciprofloxacin NN 76 77 . . . 79 82 The the DT 83 98 fluoroquinolone fluoroquinolone NN 99 109 antibiotic antibiotic NN 109 110 , , , 111 124 ciprofloxacin ciprofloxacin NN 125 126 ( ( ( 126 131 cipro cipro NN 131 132 ) ) ) 132 133 , , , 134 141 induces induce VBZ 142 157 hyperproduction hyperproduction NN 158 160 of of IN 161 172 interleukin interleukin NN 173 174 2 2 CD 175 176 ( ( ( 176 180 IL-2 il-2 NN 180 181 ) ) ) 182 185 and and CC 186 202 interferon-gamma interferon-gamma NN 203 204 ( ( ( 204 213 IFN-gamma IFN-gamma NNP 213 214 ) ) ) 215 217 in in IN 218 228 stimulated stimulate VBN 229 234 human human JJ 235 245 peripheral peripheral JJ 246 251 blood blood NN 252 263 lymphocytes lymphocyte NNS 263 264 . . . 265 267 In in IN 268 272 this this DT 273 286 investigation investigation NN 287 289 an an DT 290 298 enhanced enhance VBN 299 302 and and CC 303 312 prolonged prolong VBN 313 317 IL-2 il-2 NN 318 321 and and CC 322 326 IL-2 il-2 NN 327 331 mRNA mrna NN 332 340 response response NN 341 344 was be VBD 345 349 also also RB 350 358 detected detect VBN 359 361 in in IN 362 366 both both CC 367 377 stimulated stimulate VBN 378 379 ( ( ( 379 380 T t NN 381 385 cell cell NN 386 394 mitogens mitogen NNS 395 397 or or CC 398 410 alloantigens alloantigen NNS 410 411 ) ) ) 412 418 murine murine JJ 419 430 splenocytes splenocyte NNS 431 434 and and CC 435 437 in in IN 438 441 the the DT 442 452 stimulated stimulate VBN 453 459 murine murine JJ 460 461 T t NN 462 466 cell cell NN 467 471 line line NN 472 476 EL-4 el-4 NN 477 479 in in IN 480 483 the the DT 484 492 presence presence NN 493 495 of of IN 496 509 ciprofloxacin ciprofloxacin NN 510 511 ( ( ( 511 515 5-80 5-80 CD 516 529 micrograms/ml micrograms/ml NNS 529 530 ) ) ) 531 533 as as IN 534 542 compared compare VBN 543 545 to to TO 546 553 control control NN 554 559 cells cell NNS 560 567 without without IN 568 579 antibiotics antibiotic NNS 579 580 . . . 581 588 However however RB 588 589 , , , 590 592 in in IN 593 601 contrast contrast NN 602 604 to to TO 605 610 human human JJ 611 622 lymphocytes lymphocyte NNS 622 623 , , , 624 633 IFN-gamma IFN-gamma NNP 634 644 production production NN 645 648 was be VBD 649 658 inhibited inhibit VBN 659 662 and and CC 663 672 IFN-gamma IFN-gamma NNP 673 677 mRNA mRNA NNP 678 684 levels level NNS 685 689 were be VBD 690 700 unaffected unaffected JJ 701 703 at at IN 704 706 24 24 CD 707 708 h h NN 709 712 and and CC 713 717 only only RB 718 726 slightly slightly RB 727 738 upregulated upregulate VBN 739 741 at at IN 742 744 48 48 CD 745 748 and and CC 749 751 72 72 CD 752 753 h h NN 754 756 of of IN 757 764 culture culture NN 765 767 in in IN 768 774 murine murine JJ 775 786 splenocytes splenocyte NNS 787 796 incubated incubate VBN 797 801 with with IN 802 807 cipro cipro NN 808 809 ( ( ( 809 811 20 20 CD 812 825 micrograms/ml micrograms/ml NNS 825 826 ) ) ) 826 827 . . . 828 832 EL-4 el-4 NN 833 838 cells cell NNS 839 843 were be VBD 844 855 transfected transfecte VBN 856 860 with with IN 861 862 a a DT 863 870 plasmid plasmid NN 871 881 containing contain VBG 882 885 the the DT 886 890 IL-2 il-2 NN 891 899 promoter promoter NN 900 903 and and CC 904 912 enhancer enhancer NN 913 919 region region NN 920 926 linked link VBN 927 929 to to TO 930 933 the the DT 934 949 chloramphenicol chloramphenicol NN 950 967 acetyltransferase acetyltransferase NN 968 969 ( ( ( 969 972 CAT CAT NNP 972 973 ) ) ) 974 982 reporter reporter NN 983 987 gene gene NN 987 988 . . . 989 997 Analysis Analysis NNP 998 1000 of of IN 1001 1004 CAT CAT NNP 1005 1013 activity activity NN 1014 1022 revealed reveal VBD 1023 1027 that that IN 1028 1033 cipro cipro NN 1034 1042 enhanced enhance VBD 1043 1047 IL-2 il-2 NN 1048 1052 gene gene NN 1053 1062 induction induction NN 1062 1063 . . . 1064 1066 In in IN 1067 1075 addition addition NN 1075 1076 , , , 1077 1081 EL-4 el-4 NN 1082 1087 cells cell NNS 1088 1097 incubated incubate VBN 1098 1102 with with IN 1103 1116 ciprofloxacin ciprofloxacin NN 1117 1123 showed show VBD 1124 1126 an an DT 1127 1132 early early JJ 1133 1137 peak peak NN 1138 1141 and and CC 1142 1146 more more RBR 1147 1156 activated activate VBN 1157 1164 nuclear nuclear JJ 1165 1171 factor factor NN 1172 1174 of of IN 1175 1184 activated activate VBN 1185 1186 T t NN 1187 1192 cells cell NNS 1193 1194 ( ( ( 1194 1200 NFAT-1 nfat-1 NN 1200 1201 ) ) ) 1202 1204 as as IN 1205 1213 compared compare VBN 1214 1216 to to TO 1217 1224 control control NN 1225 1230 cells cell NNS 1231 1238 without without IN 1239 1250 antibiotics antibiotic NNS 1250 1251 . . . 1252 1257 Cipro Cipro NNP 1258 1261 did do VBD 1262 1265 not not RB 1266 1272 affect affect VB 1273 1276 the the DT 1277 1284 nuclear nuclear JJ 1285 1298 transcription transcription NN 1299 1306 factors factor NNS 1307 1311 AP-1 ap-1 NN 1312 1314 or or CC 1315 1323 NFIL-2A. NFIL-2A. NNP 1324 1329 Taken take VBN 1330 1338 together together RB 1338 1339 , , , 1340 1345 cipro cipro NN 1346 1355 inhibited inhibit VBD 1356 1365 IFN-gamma IFN-gamma NNP 1366 1375 synthesis synthesis NN 1375 1376 , , , 1377 1380 but but CC 1381 1389 enhanced enhance VBD 1390 1394 IL-2 il-2 NN 1395 1405 production production NN 1406 1408 in in IN 1409 1415 murine murine JJ 1416 1427 lymphocytes lymphocyte NNS 1428 1430 by by IN 1431 1436 means mean NNS 1437 1439 of of IN 1440 1451 influencing influence VBG 1452 1458 NFAT-1 nfat-1 NN 1459 1462 and and CC 1463 1470 causing cause VBG 1471 1473 an an DT 1474 1483 increased increase VBN 1484 1488 IL-2 il-2 NN 1489 1502 transcription transcription NN 1502 1503 . . .