0 4 IL-4 il-4 NN 5 19 down-regulates down-regulate VBZ 20 25 IL-2- il-2- NN 25 26 , , , 27 32 IL-3- il-3- NN 32 33 , , , 34 37 and and CC 38 52 GM-CSF-induced gm-csf-induced JJ 53 61 cytokine cytokine NN 62 66 gene gene NN 67 77 expression expression NN 78 80 in in IN 81 91 peripheral peripheral JJ 92 97 blood blood NN 98 107 monocytes monocyte NNS 107 108 . . . 110 114 IL-4 il-4 NN 114 115 , , , 116 117 a a DT 118 125 product product NN 126 128 of of IN 129 132 the the DT 133 141 T-helper T-helper NNP 142 143 0 0 CD 144 145 ( ( ( 145 148 Th0 th0 NN 148 149 ) ) ) 150 153 and and CC 154 155 2 2 CD 156 157 ( ( ( 157 160 Th2 th2 NN 160 161 ) ) ) 162 168 subset subset NN 168 169 , , , 170 173 was be VBD 174 184 originally originally RB 185 194 described describe VBN 195 197 as as IN 198 199 a a DT 200 206 B-cell b-cell NN 207 218 stimulatory stimulatory JJ 219 225 factor factor NN 226 229 and and CC 230 233 has have VBZ 234 246 subsequently subsequently RB 247 251 been be VBN 252 257 found find VBN 258 260 to to TO 261 269 suppress suppress VB 270 274 IL-1 il-1 NN 275 280 alpha alpha NN 280 281 , , , 282 286 IL-1 il-1 NN 287 291 beta beta NN 291 292 , , , 293 297 IL-6 IL-6 NNP 297 298 , , , 299 303 IL-8 il-8 NN 303 304 , , , 305 308 and and CC 309 318 TNF-alpha TNF-alpha NNP 319 323 gene gene NN 324 334 expression expression NN 335 337 in in IN 338 347 monocytes monocyte NNS 348 358 stimulated stimulate VBN 359 363 with with IN 364 367 LPS LPS NNP 367 368 , , , 369 372 and and CC 373 375 to to TO 376 386 upregulate upregulate VB 387 391 IL-1 il-1 NN 392 400 receptor receptor NN 401 411 antagonist antagonist NN 412 413 ( ( ( 413 419 IL1-RA IL1-RA NNP 419 420 ) ) ) 421 425 gene gene NN 426 436 expression expression NN 436 437 . . . 438 440 In in IN 441 445 this this DT 446 451 study study NN 452 454 we we PRP 455 467 investigated investigate VBD 468 471 the the DT 472 478 effect effect NN 479 481 of of IN 482 486 IL-4 il-4 NN 487 489 on on IN 490 493 the the DT 494 504 expression expression NN 505 507 of of IN 508 516 cytokine cytokine NN 517 522 genes gene NNS 523 525 in in IN 526 535 monocytes monocyte NNS 536 542 evoked evoke VBN 543 545 by by IN 546 551 other other JJ 552 560 T-helper T-helper NNP 561 565 cell cell NN 566 575 cytokines cytokine NNS 575 576 : : : 577 581 IL-2 IL-2 NNP 581 582 , , , 583 587 IL-3 IL-3 NNP 587 588 , , , 589 592 and and CC 593 599 GM-CSF GM-CSF NNP 599 600 . . . 601 605 IL-4 il-4 NN 606 620 down-regulated down-regulate VBD 621 625 mRNA mrna NN 626 638 accumulation accumulation NN 639 641 of of IN 642 645 the the DT 646 661 proinflammatory proinflammatory JJ 662 671 cytokines cytokine NNS 672 676 IL-1 il-1 NN 677 681 beta beta NN 681 682 , , , 683 687 IL-8 il-8 NN 687 688 , , , 689 692 and and CC 693 702 TNF-alpha TNF-alpha NNP 703 705 in in IN 706 715 monocytes monocyte NNS 716 726 stimulated stimulate VBN 727 731 with with IN 732 736 IL-2 il-2 NN 736 737 , , , 738 742 IL-3 IL-3 NNP 742 743 , , , 744 747 and and CC 748 754 GM-CSF GM-CSF NNP 754 755 . . . 756 760 IL-4 il-4 NN 761 765 also also RB 766 776 suppressed suppress VBD 777 780 the the DT 781 793 IL-2-induced il-2-induced JJ 794 798 IL-6 IL-6 NNP 799 803 mRNA mrna NN 804 814 expression expression NN 814 815 . . . 816 824 Temporal temporal JJ 825 833 analysis analysis NN 834 836 of of IN 837 840 the the DT 841 845 IL-4 il-4 NN 846 861 down-regulatory down-regulatory JJ 862 868 effect effect NN 869 871 on on IN 872 875 the the DT 876 881 IL-2- il-2- NN 881 882 , , , 883 888 IL-3- il-3- NN 888 889 , , , 890 892 or or CC 893 907 GM-CSF-induced gm-csf-induced JJ 908 923 proinflammatory proinflammatory JJ 924 932 cytokine cytokine NN 933 937 gene gene NN 938 948 expression expression NN 949 951 in in IN 952 961 monocytes monocyte NNS 962 970 provided provide VBD 971 979 evidence evidence NN 980 984 that that IN 985 989 IL-4 il-4 NN 990 994 acts act VBZ 995 1008 predominantly predominantly RB 1009 1011 on on IN 1012 1015 the the DT 1016 1036 post-transcriptional post-transcriptional JJ 1037 1042 level level NN 1042 1043 . . . 1044 1048 This this DT 1049 1052 was be VBD 1053 1062 supported support VBN 1063 1065 by by IN 1066 1069 the the DT 1070 1081 observation observation NN 1082 1086 that that IN 1087 1090 the the DT 1091 1106 down-regulatory down-regulatory JJ 1107 1115 capacity capacity NN 1116 1118 of of IN 1119 1123 IL-4 il-4 NN 1124 1132 appeared appear VBD 1133 1135 to to TO 1136 1138 be be VB 1139 1148 dependent dependent JJ 1149 1151 on on IN 1152 1154 de de FW 1155 1159 novo novo FW 1160 1167 protein protein NN 1168 1177 synthesis synthesis NN 1177 1178 . . . 1179 1183 IL-4 il-4 NN 1184 1187 did do VBD 1188 1191 not not RB 1192 1197 exert exert VB 1198 1209 significant significant JJ 1210 1219 influence influence NN 1220 1222 on on IN 1223 1226 the the DT 1227 1236 induction induction NN 1237 1239 of of IN 1240 1250 expression expression NN 1251 1253 of of IN 1254 1261 IL-1-RA IL-1-RA NNP 1262 1264 or or CC 1265 1272 various various JJ 1273 1277 CSFs csf NNS 1278 1280 by by IN 1281 1285 IL-2 il-2 NN 1285 1286 , , , 1287 1291 IL-3 IL-3 NNP 1291 1292 , , , 1293 1296 and and CC 1297 1303 GM-CSF GM-CSF NNP 1303 1304 . . . 1305 1306 ( ( ( 1306 1314 ABSTRACT abstract NN 1315 1324 TRUNCATED truncated VBN 1325 1327 AT at IN 1328 1331 250 250 CD 1332 1337 WORDS words NNS 1337 1338 ) ) )