0 6 ZAP-70 ZAP-70 NNP 7 15 tyrosine tyrosine NN 16 22 kinase kinase NNP 22 23 , , , 24 28 CD45 CD45 NNP 28 29 , , , 30 33 and and CC 34 35 T t NN 36 40 cell cell NN 41 49 receptor receptor NN 50 61 involvement involvement NN 62 64 in in IN 65 68 UV- UV- NNP 69 72 and and CC 73 85 H2O2-induced h2o2-induced JJ 86 87 T t NN 88 92 cell cell NN 93 99 signal signal NN 100 112 transduction transduction NN 112 113 . . . 115 122 Several several JJ 123 132 mammalian mammalian JJ 133 142 responses response NNS 143 145 to to TO 146 148 UV UV NNP 149 160 irradiation irradiation NN 160 161 , , , 162 171 including include VBG 172 175 the the DT 176 186 activation activation NN 187 189 of of IN 190 198 NF-kappa NF-kappa NNP 199 200 B B NNP 200 201 , , , 202 205 are be VBP 206 214 believed believe VBN 215 217 to to TO 218 225 involve involve VB 226 234 tyrosine tyrosine NN 235 250 phosphorylation phosphorylation NN 250 251 . . . 252 254 UV UV NNP 255 266 irradiation irradiation NN 267 270 and and CC 271 275 H2O2 h2o2 NN 276 285 treatment treatment NN 286 288 of of IN 289 290 T t NN 291 302 lymphocytes lymphocyte NNS 303 309 induce induce VBP 310 317 protein protein NN 318 326 tyrosine tyrosine NN 327 342 phosphorylation phosphorylation NN 343 346 and and CC 347 351 Ca2+ ca2+ NN 352 359 signals signal NNS 360 367 similar similar JJ 368 370 to to TO 371 376 those those DT 377 385 observed observe VBN 386 395 following follow VBG 396 406 biological biological JJ 407 418 stimulation stimulation NN 418 419 . . . 420 422 We we PRP 423 427 have have VBP 428 436 examined examine VBN 437 440 the the DT 441 445 role role NN 446 448 of of IN 449 453 cell cell NN 454 461 surface surface NN 462 471 molecules molecule NNS 472 474 in in IN 475 480 these these DT 481 490 responses response NNS 490 491 . . . 492 498 Normal normal JJ 499 500 T t NN 501 512 lymphocytes lymphocyte NNS 513 518 whose whose WP$ 519 526 surface surface NN 527 537 expression expression NN 538 540 of of IN 541 544 CD3 CD3 NNP 545 548 was be VBD 549 557 depleted deplete VBN 558 564 showed show VBD 565 573 impaired impaired JJ 574 584 UV-induced uv-induced JJ 585 593 tyrosine tyrosine NN 594 609 phosphorylation phosphorylation NN 610 613 and and CC 614 618 Ca2+ ca2+ NN 619 626 signals signal NNS 626 627 . . . 628 637 Similarly similarly RB 637 638 , , , 639 645 Jurkat Jurkat NNP 646 647 T t NN 648 652 cell cell NN 653 658 lines line NNS 659 668 deficient deficient JJ 669 671 in in IN 672 675 CD3 CD3 NNP 676 678 or or CC 679 683 CD45 CD45 NNP 684 694 expression expression NN 695 699 also also RB 700 704 gave give VBD 705 713 impaired impaired JJ 714 716 UV UV NNP 717 726 responses response NNS 726 727 . . . 728 735 However however RB 735 736 , , , 737 740 all all PDT 741 746 these these DT 747 751 cell cell NN 752 757 types type NNS 758 763 still still RB 764 768 gave give VBD 769 775 strong strong JJ 776 780 Ca2+ ca2+ NN 781 784 and and CC 785 793 tyrosine tyrosine NN 794 809 phosphorylation phosphorylation NN 810 819 responses response NNS 820 822 to to TO 823 827 H2O2 h2o2 NN 827 828 . . . 829 832 The the DT 833 834 T t NN 835 839 cell cell NN 840 848 tyrosine tyrosine NN 849 855 kinase kinase NNP 856 862 ZAP-70 ZAP-70 NNP 863 866 was be VBD 867 872 found find VBN 873 875 to to TO 876 878 be be VB 879 885 highly highly RB 886 896 responsive responsive JJ 897 899 to to TO 900 902 UV UV NNP 903 906 and and CC 907 911 H2O2 h2o2 NN 912 921 treatment treatment NN 921 922 . . . 923 929 ZAP-70 ZAP-70 NNP 930 944 responsiveness responsiveness NN 945 947 to to TO 948 950 UV UV NNP 951 959 required require VBD 960 970 expression expression NN 971 973 of of IN 974 978 both both CC 979 982 CD3 CD3 NNP 983 986 and and CC 987 991 CD45 CD45 NNP 991 992 , , , 993 1000 whereas whereas IN 1001 1005 only only RB 1006 1009 CD3 cd3 NN 1010 1013 was be VBD 1014 1022 required require VBN 1023 1026 for for IN 1027 1030 the the DT 1031 1039 response response NN 1040 1042 to to TO 1043 1047 H2O2 h2o2 NN 1047 1048 . . . 1049 1059 UV-induced uv-induced JJ 1060 1070 activation activation NN 1071 1073 of of IN 1074 1082 NF-kappa NF-kappa NNP 1083 1084 B B NNP 1085 1088 was be VBD 1089 1096 blocked block VBN 1097 1099 by by IN 1100 1103 CD3 cd3 NN 1104 1113 depletion depletion NN 1113 1114 , , , 1115 1125 indicating indicate VBG 1126 1129 the the DT 1130 1140 importance importance NN 1141 1143 of of IN 1144 1148 such such JJ 1149 1153 cell cell NN 1154 1161 surface surface NN 1162 1171 molecules molecule NNS 1172 1174 in in IN 1175 1185 biological biological JJ 1186 1195 responses response NNS 1196 1198 to to TO 1199 1201 UV UV NNP 1201 1202 . . . 1203 1205 In in IN 1206 1217 nonlymphoid nonlymphoid JJ 1218 1223 cells cell NNS 1223 1224 , , , 1225 1228 the the DT 1229 1238 epidermal epidermal JJ 1239 1245 growth growth NN 1246 1252 factor factor NN 1253 1261 receptor receptor NN 1262 1271 displayed display VBD 1272 1281 increased increase VBN 1282 1290 tyrosine tyrosine NN 1291 1306 phosphorylation phosphorylation NN 1307 1313 within within IN 1314 1321 seconds second NNS 1322 1324 of of IN 1325 1327 UV UV NNP 1328 1339 irradiation irradiation NN 1339 1340 . . . 1341 1346 These these DT 1347 1354 results result NNS 1355 1362 suggest suggest VBP 1363 1367 that that IN 1368 1378 UV-induced uv-induced JJ 1379 1385 signal signal NN 1386 1398 transduction transduction NN 1399 1401 is be VBZ 1402 1410 mediated mediate VBN 1411 1414 via via IN 1415 1419 cell cell NN 1420 1427 surface surface NN 1428 1437 receptors receptor NNS 1438 1442 that that WDT 1443 1451 normally normally RB 1452 1459 respond respond VBP 1460 1462 to to TO 1463 1473 biological biological JJ 1474 1485 stimulation stimulation NN 1485 1486 , , , 1487 1494 whereas whereas IN 1495 1499 H2O2 h2o2 NN 1500 1502 is be VBZ 1503 1507 able able JJ 1508 1510 to to TO 1511 1520 partially partially RB 1521 1527 bypass bypass VB 1528 1532 this this DT 1533 1544 requirement requirement NN 1544 1545 . . .