0	8	Positive	positive	JJ
9	12	and	and	CC
13	21	negative	negative	JJ
22	32	regulation	regulation	NN
33	35	of	of	IN
36	40	IL-2	il-2	NN
41	45	gene	gene	NN
46	56	expression	expression	NN
56	57	:	:	:
58	62	role	role	NN
63	65	of	of	IN
66	74	multiple	multiple	JJ
75	85	regulatory	regulatory	JJ
86	91	sites	site	NNS
91	92	.	.	.

94	105	Interleukin	Interleukin	NNP
106	107	2	2	CD
108	109	(	(	(
109	113	IL-2	il-2	NN
113	114	)	)	)
115	117	is	be	VBZ
118	120	an	an	DT
121	130	important	important	JJ
131	141	lymphokine	lymphokine	NN
142	150	required	require	VBN
151	153	in	in	IN
154	157	the	the	DT
158	165	process	process	NN
166	168	of	of	IN
169	170	T	t	NN
171	175	cell	cell	NN
176	186	activation	activation	NN
186	187	,	,	,
188	201	proliferation	proliferation	NN
201	202	,	,	,
203	209	clonal	clonal	JJ
210	219	expansion	expansion	NN
220	223	and	and	CC
224	239	differentiation	differentiation	NN
239	240	.	.	.

241	244	The	the	DT
245	249	IL-2	il-2	NN
250	254	gene	gene	NN
255	263	displays	display	VBZ
264	268	both	both	CC
269	270	T	t	NN
271	275	cell	cell	NN
276	284	specific	specific	JJ
285	288	and	and	CC
289	298	inducible	inducible	JJ
299	309	expression	expression	NN
309	310	:	:	:
311	313	it	it	PRP
314	316	is	be	VBZ
317	321	only	only	RB
322	331	expressed	express	VBN
332	334	in	in	IN
335	339	CD4+	cd4+	JJ
340	341	T	t	NN
342	347	cells	cell	NNS
348	353	after	after	IN
354	363	antigenic	antigenic	JJ
364	366	or	or	CC
367	376	mitogenic	mitogenic	JJ
377	388	stimulation	stimulation	NN
388	389	.	.	.

390	397	Several	several	JJ
398	408	cis-acting	cis-acting	JJ
409	419	regulatory	regulatory	JJ
420	425	sites	site	NNS
426	429	are	be	VBP
430	438	required	require	VBN
439	442	for	for	IN
443	452	induction	induction	NN
453	455	of	of	IN
456	459	the	the	DT
460	464	IL-2	il-2	NN
465	469	gene	gene	NN
470	475	after	after	IN
476	487	stimulation	stimulation	NN
487	488	.	.	.

489	491	In	in	IN
492	496	this	this	DT
497	502	study	study	NN
502	503	,	,	,
504	506	we	we	PRP
507	511	have	have	VBP
512	520	analysed	analyse	VBN
521	524	the	the	DT
525	533	function	function	NN
534	536	of	of	IN
537	542	these	these	DT
543	553	cis-acting	cis-acting	JJ
554	564	regulatory	regulatory	JJ
565	570	sites	site	NNS
571	573	in	in	IN
574	577	the	the	DT
578	585	context	context	NN
586	588	of	of	IN
589	592	the	the	DT
593	599	native	native	JJ
600	604	IL-2	il-2	NN
605	613	enhancer	enhancer	NN
614	617	and	and	CC
618	626	promoter	promoter	NN
627	635	sequence	sequence	NN
635	636	.	.	.

637	640	The	the	DT
641	648	results	result	NNS
649	651	of	of	IN
652	656	this	this	DT
657	662	study	study	NN
663	670	suggest	suggest	VBP
671	675	that	that	IN
676	679	the	the	DT
680	684	NFAT	NFAT	NNP
685	686	(	(	(
686	690	-276	-276	CD
691	693	to	to	TO
694	698	-261	-261	CD
698	699	)	)	)
699	700	,	,	,
701	704	the	the	DT
705	711	distal	distal	JJ
712	719	octamer	octamer	NN
720	721	(	(	(
721	725	-256	-256	CD
726	728	to	to	TO
729	733	-248	-248	CD
733	734	)	)	)
735	738	and	and	CC
739	742	the	the	DT
743	751	proximal	proximal	JJ
752	759	octamer	octamer	NN
760	761	(	(	(
761	764	-75	-75	CD
765	767	to	to	TO
768	771	-66	-66	CD
771	772	)	)	)
773	778	sites	site	NNS
779	782	not	not	RB
783	787	only	only	RB
788	791	act	act	VBP
792	794	as	as	IN
795	804	enhancers	enhancer	NNS
805	807	of	of	IN
808	812	IL-2	il-2	NN
813	817	gene	gene	NN
818	831	transcription	transcription	NN
832	834	in	in	IN
835	838	the	the	DT
839	847	presence	presence	NN
848	850	of	of	IN
851	859	cellular	cellular	JJ
860	871	stimulation	stimulation	NN
871	872	,	,	,
873	876	but	but	CC
877	881	also	also	RB
882	886	have	have	VBP
887	888	a	a	DT
889	898	silencing	silencing	JJ
899	905	effect	effect	NN
906	908	on	on	IN
909	913	IL-2	il-2	NN
914	918	gene	gene	NN
919	929	expression	expression	NN
930	932	in	in	IN
933	940	resting	rest	VBG
941	946	cells	cell	NNS
946	947	.	.	.

948	951	Two	two	CD
952	957	other	other	JJ
958	963	sites	site	NNS
964	971	display	display	VBP
972	981	disparate	disparate	JJ
982	989	effects	effect	NNS
990	992	on	on	IN
993	997	IL-2	il-2	NN
998	1002	gene	gene	NN
1003	1013	expression	expression	NN
1014	1016	in	in	IN
1017	1026	different	different	JJ
1027	1028	T	t	NN
1029	1037	leukemia	leukemia	NN
1038	1042	cell	cell	NN
1043	1048	lines	line	NNS
1048	1049	:	:	:
1050	1053	the	the	DT
1054	1060	distal	distal	JJ
1061	1067	purine	purine	NN
1068	1071	box	box	NN
1072	1073	(	(	(
1073	1077	-291	-291	CD
1078	1080	to	to	TO
1081	1085	-277	-277	CD
1085	1086	)	)	)
1087	1090	and	and	CC
1091	1094	the	the	DT
1095	1103	proximal	proximal	JJ
1104	1110	purine	purine	NN
1111	1114	box	box	NN
1115	1120	sites	site	NNS
1121	1122	(	(	(
1122	1126	-145	-145	CD
1127	1129	to	to	TO
1130	1136	-128).	-128).	CD
1137	1144	Finally	finally	RB
1144	1145	,	,	,
1146	1149	the	the	DT
1150	1154	AP-1	ap-1	NN
1155	1156	(	(	(
1156	1160	-186	-186	CD
1161	1163	to	to	TO
1164	1168	-176	-176	CD
1168	1169	)	)	)
1170	1173	and	and	CC
1174	1177	the	the	DT
1178	1183	kappa	kappa	NN
1184	1185	B	B	NNP
1186	1191	sites	site	NNS
1192	1193	(	(	(
1193	1197	-206	-206	CD
1198	1200	to	to	TO
1201	1205	-195	-195	CD
1205	1206	)	)	)
1207	1214	respond	respond	VBP
1215	1217	to	to	TO
1218	1227	different	different	JJ
1228	1236	cellular	cellular	JJ
1237	1247	activation	activation	NN
1248	1250	in	in	IN
1251	1254	EL4	el4	NN
1255	1260	cells	cell	NNS
1260	1261	.	.	.

1262	1265	The	the	DT
1266	1270	AP-1	ap-1	NN
1271	1275	site	site	NN
1276	1284	mediated	mediate	VBD
1285	1288	the	the	DT
1289	1297	response	response	NN
1298	1300	to	to	TO
1301	1304	PMA	PMA	NNP
1305	1316	stimulation	stimulation	NN
1317	1322	while	while	IN
1323	1326	the	the	DT
1327	1332	kappa	kappa	NN
1333	1334	B	B	NNP
1335	1339	site	site	NN
1340	1349	responded	respond	VBD
1350	1352	to	to	TO
1353	1357	IL-1	il-1	NN
1358	1369	stimulation	stimulation	NN
1369	1370	.	.	.

1371	1376	These	these	DT
1377	1381	data	datum	NNS
1382	1389	suggest	suggest	VBP
1390	1394	that	that	IN
1395	1398	the	the	DT
1399	1409	regulation	regulation	NN
1410	1412	of	of	IN
1413	1417	IL-2	il-2	NN
1418	1422	gene	gene	NN
1423	1433	expression	expression	NN
1434	1436	is	be	VBZ
1437	1438	a	a	DT
1439	1446	complex	complex	JJ
1447	1454	process	process	NN
1455	1458	and	and	CC
1459	1467	multiple	multiple	JJ
1468	1478	cis-acting	cis-acting	JJ
1479	1489	regulatory	regulatory	JJ
1490	1495	sites	site	NNS
1496	1504	interact	interact	VBP
1505	1507	to	to	TO
1508	1513	exert	exert	VB
1514	1523	different	different	JJ
1524	1531	effects	effect	NNS
1532	1534	in	in	IN
1535	1536	T	t	NN
1537	1542	cells	cell	NNS
1543	1557	representative	representative	JJ
1558	1560	of	of	IN
1561	1572	alternative	alternative	JJ
1573	1579	stages	stage	NNS
1580	1582	of	of	IN
1583	1598	differentiation	differentiation	NN
1598	1599	.	.	.