0	7	Signals	signal	NNS
8	11	and	and	CC
12	19	nuclear	nuclear	JJ
20	27	factors	factor	NNS
28	32	that	that	WDT
33	41	regulate	regulate	VBP
42	45	the	the	DT
46	56	expression	expression	NN
57	59	of	of	IN
60	73	interleukin-4	interleukin-4	NN
74	77	and	and	CC
78	91	interleukin-5	interleukin-5	NN
92	97	genes	gene	NNS
98	100	in	in	IN
101	107	helper	helper	NN
108	109	T	t	NN
110	115	cells	cell	NNS
115	116	.	.	.

118	123	Mouse	Mouse	NNP
124	131	thymoma	thymoma	NN
132	136	line	line	NN
137	141	EL-4	el-4	NN
142	147	cells	cell	NNS
148	155	produce	produce	VBP
156	165	cytokines	cytokine	NNS
166	170	such	such	JJ
171	173	as	as	IN
174	185	interleukin	interleukin	NN
186	187	(	(	(
187	192	IL)-2	IL)-2	NNP
192	193	,	,	,
194	198	IL-3	IL-3	NNP
198	199	,	,	,
200	204	IL-4	il-4	NN
204	205	,	,	,
206	211	IL-10	IL-10	NNP
211	212	,	,	,
213	216	and	and	CC
217	239	granulocyte-macrophage	granulocyte-macrophage	JJ
240	258	colony-stimulating	colony-stimulating	JJ
259	265	factor	factor	NN
266	268	in	in	IN
269	277	response	response	NN
278	280	to	to	TO
281	288	phorbol	phorbol	NN
289	301	12-myristate	12-myristate	NN
302	312	13-acetate	13-acetate	NN
313	314	(	(	(
314	317	PMA	PMA	NNP
317	318	)	)	)
318	319	.	.	.

320	324	EL-4	el-4	NN
325	330	cells	cell	NNS
331	335	also	also	RB
336	343	produce	produce	VBP
344	347	low	low	JJ
348	354	levels	level	NNS
355	357	of	of	IN
358	362	IL-5	il-5	NN
363	367	when	when	WRB
368	378	stimulated	stimulate	VBN
379	381	by	by	IN
382	385	PMA	PMA	NNP
386	391	alone	alone	RB
391	392	;	;	:
393	400	however	however	RB
400	401	,	,	,
402	406	cAMP	cAMP	NNP
407	414	greatly	greatly	RB
415	423	augments	augment	VBZ
424	437	PMA-dependent	pma-dependent	JJ
438	442	IL-5	IL-5	NNP
443	453	production	production	NN
453	454	.	.	.

455	456	A	a	DT
457	466	transient	transient	JJ
467	479	transfection	transfection	NN
480	485	assay	assay	NN
486	494	revealed	reveal	VBD
495	499	that	that	IN
500	503	two	two	CD
504	511	signals	signal	NNS
511	512	,	,	,
513	516	PMA	PMA	NNP
517	520	and	and	CC
521	525	cAMP	cAMP	NNP
525	526	,	,	,
527	530	are	be	VBP
531	539	required	require	VBN
540	543	for	for	IN
544	551	optimal	optimal	JJ
552	562	activation	activation	NN
563	565	of	of	IN
566	569	the	the	DT
570	574	IL-5	il-5	NN
575	583	promoter	promoter	NN
583	584	.	.	.

585	587	In	in	IN
588	596	contrast	contrast	NN
596	597	,	,	,
598	602	cAMP	camp	NN
603	609	almost	almost	RB
610	620	completely	completely	RB
621	630	inhibited	inhibit	VBD
631	634	the	the	DT
635	648	PMA-dependent	pma-dependent	JJ
649	659	activation	activation	NN
660	662	of	of	IN
663	666	the	the	DT
667	677	endogenous	endogenous	JJ
678	682	IL-2	il-2	NN
683	687	gene	gene	NN
687	688	,	,	,
689	691	as	as	RB
692	696	well	well	RB
697	699	as	as	IN
700	703	the	the	DT
704	715	transfected	transfecte	VBN
716	720	IL-2	il-2	NN
721	729	promoter	promoter	NN
729	730	.	.	.

731	736	These	these	DT
737	744	results	result	NNS
745	753	indicate	indicate	VBP
754	758	that	that	IN
759	762	the	the	DT
763	767	IL-5	IL-5	NNP
768	772	gene	gene	NN
773	775	is	be	VBZ
776	786	positively	positively	RB
787	796	regulated	regulate	VBN
797	799	by	by	IN
800	804	cAMP	camp	NN
805	807	in	in	IN
808	809	a	a	DT
810	816	manner	manner	NN
817	825	opposite	opposite	JJ
826	828	to	to	TO
829	833	that	that	DT
834	837	for	for	IN
838	841	the	the	DT
842	846	IL-2	il-2	NN
847	851	gene	gene	NN
851	852	.	.	.

853	856	One	one	CD
857	859	of	of	IN
860	863	the	the	DT
864	871	nuclear	nuclear	JJ
872	879	factors	factor	NNS
880	881	(	(	(
881	884	NFs	NF	NNP
884	885	)	)	)
886	890	that	that	WDT
891	900	regulates	regulate	VBZ
901	904	the	the	DT
905	913	response	response	NN
914	916	of	of	IN
917	920	the	the	DT
921	925	IL-5	IL-5	NNP
926	934	promoter	promoter	NN
935	937	to	to	TO
938	942	cAMP	cAMP	NNP
943	946	and	and	CC
947	950	PMA	PMA	NNP
951	954	has	have	VBZ
955	965	properties	property	NNS
966	973	similar	similar	JJ
974	976	to	to	TO
977	979	NF	NF	NNP
980	983	for	for	IN
984	993	activated	activate	VBN
994	995	t	t	NN
996	1000	cell	cell	NN
1000	1001	.	.	.

1002	1005	The	the	DT
1006	1007	P	p	NN
1008	1016	sequence	sequence	NN
1017	1019	of	of	IN
1020	1023	the	the	DT
1024	1028	IL-4	il-4	NN
1029	1033	gene	gene	NN
1033	1034	,	,	,
1035	1042	defined	define	VBN
1043	1045	as	as	IN
1046	1047	a	a	DT
1048	1058	responsive	responsive	JJ
1059	1066	element	element	NN
1067	1070	for	for	IN
1071	1074	PMA	PMA	NNP
1075	1078	and	and	CC
1079	1086	calcium	calcium	NN
1087	1096	ionophore	ionophore	NN
1097	1098	(	(	(
1098	1104	A23187	A23187	NNP
1104	1105	)	)	)
1105	1106	,	,	,
1107	1113	shares	share	VBZ
1114	1122	sequence	sequence	NN
1123	1133	similarity	similarity	NN
1134	1138	with	with	IN
1139	1142	the	the	DT
1143	1145	NF	NF	NNP
1146	1151	kappa	kappa	NN
1152	1153	B	B	NNP
1154	1157	and	and	CC
1158	1161	the	the	DT
1162	1174	NF-activated	nf-activated	JJ
1175	1176	T	t	NN
1177	1181	cell	cell	NN
1182	1189	binding	binding	NN
1190	1195	sites	site	NNS
1195	1196	.	.	.

1197	1199	We	we	PRP
1200	1209	attempted	attempt	VBD
1210	1212	to	to	TO
1213	1222	determine	determine	VB
1223	1230	whether	whether	IN
1231	1236	NF(P)	NF(P)	NNP
1236	1237	,	,	,
1238	1239	a	a	DT
1240	1247	nuclear	nuclear	JJ
1248	1254	factor	factor	NN
1255	1263	specific	specific	JJ
1264	1267	for	for	IN
1268	1271	the	the	DT
1272	1273	P	p	NN
1274	1282	sequence	sequence	NN
1282	1283	,	,	,
1284	1286	is	be	VBZ
1287	1294	related	related	JJ
1295	1297	to	to	TO
1298	1306	NF-kappa	NF-kappa	NNP
1307	1308	B	B	NNP
1309	1312	and	and	CC
1313	1320	nuclear	nuclear	JJ
1321	1327	factor	factor	NN
1328	1331	for	for	IN
1332	1341	activated	activate	VBN
1342	1343	T	t	NN
1344	1348	cell	cell	NN
1349	1350	(	(	(
1350	1355	NF-AT	NF-AT	NNP
1355	1356	)	)	)
1356	1357	.	.	.

1358	1360	In	in	IN
1361	1376	electromobility	electromobility	NN
1377	1382	shift	shift	NN
1383	1389	assays	assay	NNS
1390	1394	both	both	CC
1395	1403	NF-kappa	NF-kappa	NNP
1404	1405	B	B	NNP
1406	1407	(	(	(
1407	1410	P65	P65	NNP
1411	1413	or	or	CC
1414	1421	P65/P50	P65/P50	NNP
1422	1433	heterodimer	heterodimer	NN
1433	1434	)	)	)
1435	1438	and	and	CC
1439	1444	NF-AT	NF-AT	NNP
1445	1450	bound	bind	VBD
1451	1453	to	to	TO
1454	1457	the	the	DT
1458	1459	P	p	NN
1460	1468	sequence	sequence	NN
1468	1469	.	.	.

1470	1477	However	however	RB
1477	1478	,	,	,
1479	1487	sequence	sequence	NN
1488	1499	specificity	specificity	NN
1500	1502	of	of	IN
1503	1508	NF-AT	NF-AT	NNP
1509	1512	was	be	VBD
1513	1517	more	more	RBR
1518	1525	similar	similar	JJ
1526	1528	to	to	TO
1529	1533	that	that	DT
1534	1536	of	of	IN
1537	1542	NF(P)	nf(p)	NN
1542	1543	,	,	,
1544	1547	and	and	CC
1548	1552	only	only	RB
1553	1554	a	a	DT
1555	1560	small	small	JJ
1561	1567	amount	amount	NN
1568	1570	of	of	IN
1571	1574	P65	P65	NNP
1575	1578	was	be	VBD
1579	1587	detected	detect	VBN
1588	1590	in	in	IN
1591	1596	NF(P)	NF(P)	NNP
1596	1597	.	.	.

1598	1603	These	these	DT
1604	1611	results	result	NNS
1612	1620	indicate	indicate	VBP
1621	1625	that	that	IN
1626	1627	a	a	DT
1628	1637	component	component	NN
1638	1640	or	or	CC
1641	1651	components	component	NNS
1652	1654	of	of	IN
1655	1660	NF-AT	NF-AT	NNP
1661	1665	have	have	VBP
1666	1669	the	the	DT
1670	1679	potential	potential	NN
1680	1682	to	to	TO
1683	1695	reconstitute	reconstitute	VB
1696	1701	NF(P)	nf(p)	NN
1701	1702	,	,	,
1703	1710	whereas	whereas	IN
1711	1719	NF-kappa	NF-kappa	NNP
1720	1721	B	B	NNP
1722	1727	alone	alone	RB
1728	1732	does	do	VBZ
1733	1736	not	not	RB
1737	1744	account	account	VB
1745	1748	for	for	IN
1749	1754	NF(P)	NF(P)	NNP
1755	1757	in	in	IN
1758	1764	Jurkat	Jurkat	NNP
1765	1770	crude	crude	JJ
1771	1778	extract	extract	NN
1778	1779	.	.	.

1780	1785	Taken	take	VBN
1786	1794	together	together	RB
1794	1795	,	,	,
1796	1801	these	these	DT
1802	1809	results	result	NNS
1810	1817	suggest	suggest	VBP
1818	1822	that	that	IN
1823	1833	NF-AT-like	nf-at-like	JJ
1834	1841	factors	factor	NNS
1842	1845	are	be	VBP
1846	1854	involved	involve	VBN
1855	1857	in	in	IN
1858	1861	the	the	DT
1862	1872	regulation	regulation	NN
1873	1875	of	of	IN
1876	1880	IL-4	il-4	NN
1881	1884	and	and	CC
1885	1889	IL-5	il-5	NN
1890	1895	genes	gene	NNS
1895	1896	.	.	.