0	16	Characterization	characterization	NN
17	19	of	of	IN
20	25	NF(P)	NF(P)	NNP
25	26	,	,	,
27	30	the	the	DT
31	38	nuclear	nuclear	JJ
39	45	factor	factor	NN
46	50	that	that	WDT
51	60	interacts	interact	VBZ
61	65	with	with	IN
66	69	the	the	DT
70	80	regulatory	regulatory	JJ
81	82	P	p	NN
83	91	sequence	sequence	NN
92	93	(	(	(
93	109	5’-CGAAAATTTCC-3	5'-CGAAAATTTCC-3	NNP
109	110	’	'	''
110	111	)	)	)
112	114	of	of	IN
115	118	the	the	DT
119	124	human	human	JJ
125	138	interleukin-4	interleukin-4	NN
139	143	gene	gene	NN
143	144	:	:	:
145	157	relationship	relationship	NN
158	160	to	to	TO
161	169	NF-kappa	NF-kappa	NNP
170	171	B	B	NNP
172	175	and	and	CC
176	181	NF-AT	NF-AT	NNP
181	182	.	.	.

184	187	The	the	DT
188	189	P	p	NN
190	198	sequence	sequence	NN
199	201	of	of	IN
202	205	the	the	DT
206	211	human	human	JJ
212	225	interleukin-4	interleukin-4	NN
226	227	(	(	(
227	231	IL-4	il-4	NN
231	232	)	)	)
233	237	gene	gene	NN
237	238	,	,	,
239	244	which	which	WDT
245	248	was	be	VBD
249	256	defined	define	VBN
257	259	as	as	IN
260	261	a	a	DT
262	272	responsive	responsive	JJ
273	280	element	element	NN
281	284	for	for	IN
285	292	phorbol	phorbol	NN
293	305	12-myristate	12-myristate	NN
306	316	13-acetate	13-acetate	NN
317	320	and	and	CC
321	328	calcium	calcium	NN
329	338	ionophore	ionophore	NN
339	340	(	(	(
340	346	A23187	a23187	NN
346	347	)	)	)
348	350	in	in	IN
351	357	Jurkat	Jurkat	NNP
358	359	T	t	NN
360	365	cells	cell	NNS
365	366	,	,	,
367	373	shares	share	VBZ
374	382	sequence	sequence	NN
383	393	similarity	similarity	NN
394	398	with	with	IN
399	402	the	the	DT
403	411	NF-kappa	NF-kappa	NNP
412	413	B	B	NNP
414	417	and	and	CC
418	421	the	the	DT
422	427	NF-AT	nf-at	NN
428	435	binding	binding	NN
436	441	sites	site	NNS
441	442	.	.	.

443	445	We	we	PRP
446	454	examined	examine	VBD
455	462	whether	whether	IN
463	468	NF(P)	NF(P)	NNP
468	469	,	,	,
470	471	a	a	DT
472	479	nuclear	nuclear	JJ
480	486	factor	factor	NN
487	495	specific	specific	JJ
496	499	for	for	IN
500	503	the	the	DT
504	505	P	p	NN
506	514	sequence	sequence	NN
514	515	,	,	,
516	518	is	be	VBZ
519	526	related	related	JJ
527	529	to	to	TO
530	538	NF-kappa	NF-kappa	NNP
539	540	B	B	NNP
541	544	and	and	CC
545	550	NF-AT	NF-AT	NNP
550	551	.	.	.

552	560	NF-kappa	NF-kappa	NNP
561	562	B	B	NNP
563	564	(	(	(
564	567	P65	P65	NNP
568	570	or	or	CC
571	578	P65/P50	P65/P50	NNP
579	590	heterodimer	heterodimer	NN
590	591	)	)	)
592	597	bound	bind	VBD
598	600	to	to	TO
601	604	the	the	DT
605	606	P	p	NN
607	615	sequence	sequence	NN
616	618	in	in	IN
619	634	electrophoretic	electrophoretic	JJ
635	643	mobility	mobility	NN
644	649	shift	shift	NN
650	656	assays	assay	NNS
657	658	(	(	(
658	662	EMSA	EMSA	NNP
662	663	)	)	)
664	667	and	and	CC
668	677	activated	activate	VBD
678	691	transcription	transcription	NN
692	699	through	through	IN
700	703	the	the	DT
704	705	P	p	NN
706	714	sequence	sequence	NN
715	719	when	when	WRB
720	730	expression	expression	NN
731	739	plasmids	plasmid	NNS
740	744	were	be	VBD
745	758	cotransfected	cotransfecte	VBN
759	763	with	with	IN
764	765	P	p	NN
766	781	sequence-driven	sequence-driven	JJ
782	790	reporter	reporter	NN
791	799	plasmids	plasmid	NNS
800	802	in	in	IN
803	809	Jurkat	Jurkat	NNP
810	811	T	t	NN
812	817	cells	cell	NNS
817	818	.	.	.

819	821	In	in	IN
822	827	EMSAs	EMSA	NNP
827	828	,	,	,
829	834	NF(P)	nf(p)	NN
835	842	binding	binding	NN
843	846	was	be	VBD
847	856	inhibited	inhibit	VBN
857	859	by	by	IN
860	863	the	the	DT
864	873	unlabeled	unlabeled	JJ
874	879	NF-AT	nf-at	NN
880	887	binding	binding	NN
888	892	site	site	NN
893	896	but	but	CC
897	900	not	not	RB
901	903	by	by	IN
904	907	the	the	DT
908	917	unlabeled	unlabeled	JJ
918	921	AP1	ap1	NN
922	929	binding	binding	NN
930	934	site	site	NN
935	938	and	and	CC
939	947	purified	purify	VBN
948	953	NF-AT	NF-AT	NNP
954	963	contained	contain	VBD
964	966	an	an	DT
967	975	activity	activity	NN
976	980	that	that	WDT
981	986	bound	bind	VBD
987	989	to	to	TO
990	993	the	the	DT
994	995	P	p	NN
996	1004	sequence	sequence	NN
1004	1005	.	.	.

1006	1010	Both	both	CC
1011	1019	mobility	mobility	NN
1020	1025	shift	shift	NN
1026	1029	and	and	CC
1030	1038	sequence	sequence	NN
1039	1050	specificity	specificity	NN
1051	1053	of	of	IN
1054	1059	NF-AT	NF-AT	NNP
1060	1064	were	be	VBD
1065	1072	similar	similar	JJ
1073	1075	to	to	TO
1076	1081	those	those	DT
1082	1084	of	of	IN
1085	1090	NF(P)	NF(P)	NNP
1091	1094	and	and	CC
1095	1099	only	only	RB
1100	1101	a	a	DT
1102	1107	small	small	JJ
1108	1114	amount	amount	NN
1115	1117	of	of	IN
1118	1121	P65	P65	NNP
1122	1125	was	be	VBD
1126	1134	detected	detect	VBN
1135	1137	in	in	IN
1138	1143	NF(P)	nf(p)	NN
1144	1146	in	in	IN
1147	1152	crude	crude	JJ
1153	1160	nuclear	nuclear	JJ
1161	1169	extracts	extract	NNS
1169	1170	.	.	.

1171	1176	These	these	DT
1177	1184	results	result	NNS
1185	1193	indicate	indicate	VBP
1194	1198	that	that	IN
1199	1202	the	the	DT
1203	1215	component(s)	component(s)	NN
1216	1218	of	of	IN
1219	1224	NF-AT	NF-AT	NNP
1225	1228	has	have	VBZ
1229	1232	the	the	DT
1233	1242	potential	potential	NN
1243	1245	to	to	TO
1246	1258	reconstitute	reconstitute	VB
1259	1264	NF(P)	nf(p)	NN
1265	1272	whereas	whereas	IN
1273	1281	NF-kappa	NF-kappa	NNP
1282	1283	B	B	NNP
1284	1289	alone	alone	RB
1290	1296	cannot	cannot	MD
1297	1304	account	account	VB
1305	1308	for	for	IN
1309	1314	NF(P)	nf(p)	NN
1315	1317	in	in	IN
1318	1323	crude	crude	JJ
1324	1332	extracts	extract	NNS
1332	1333	.	.	.

1334	1340	Unlike	unlike	IN
1341	1346	NF-AT	NF-AT	NNP
1346	1347	,	,	,
1348	1353	NF(P)	NF(P)	NNP
1354	1358	does	do	VBZ
1359	1362	not	not	RB
1363	1370	contain	contain	VB
1371	1374	AP1	ap1	NN
1375	1377	as	as	IN
1378	1381	its	its	PRP$
1382	1385	DNA	dna	NN
1386	1393	binding	binding	NN
1394	1403	component	component	NN
1403	1404	.	.	.