0	5	Human	human	JJ
6	22	immunodeficiency	immunodeficiency	NN
23	28	virus	virus	NN
29	33	type	type	NN
34	35	1	1	CD
36	39	Nef	nef	NN
40	47	protein	protein	NN
48	62	down-regulates	down-regulate	VBZ
63	76	transcription	transcription	NN
77	84	factors	factor	NNS
85	93	NF-kappa	NF-kappa	NNP
94	95	B	B	NNP
96	99	and	and	CC
100	104	AP-1	ap-1	NN
105	107	in	in	IN
108	113	human	human	JJ
114	115	T	t	NN
116	121	cells	cell	NNS
122	124	in	in	FW
125	130	vitro	vitro	FW
131	136	after	after	IN
137	143	T-cell	t-cell	NN
144	152	receptor	receptor	NN
153	164	stimulation	stimulation	NN
164	165	.	.	.

167	172	Human	human	JJ
173	189	immunodeficiency	immunodeficiency	NN
190	195	virus	virus	NN
196	200	type	type	NN
201	202	1	1	CD
203	204	(	(	(
204	209	HIV-1	HIV-1	NNP
209	210	)	)	)
211	219	negative	negative	JJ
220	226	factor	factor	NN
227	228	(	(	(
228	231	Nef	Nef	NNP
231	232	)	)	)
233	236	has	have	VBZ
237	241	been	be	VBN
242	247	shown	show	VBN
248	250	to	to	TO
251	264	down-regulate	down-regulate	VB
265	268	the	the	DT
269	282	transcription	transcription	NN
283	290	factors	factor	NNS
291	299	NF-kappa	NF-kappa	NNP
300	301	B	B	NNP
302	305	and	and	CC
306	310	AP-1	ap-1	NN
311	313	in	in	FW
314	319	vitro	vitro	FW
319	320	.	.	.

321	323	To	to	TO
324	330	define	define	VB
331	334	the	the	DT
335	344	mechanism	mechanism	NN
345	347	of	of	IN
348	354	action	action	NN
355	357	of	of	IN
358	361	the	the	DT
362	365	Nef	Nef	NNP
366	373	protein	protein	NN
373	374	,	,	,
375	378	the	the	DT
379	385	signal	signal	NN
386	398	transduction	transduction	NN
399	407	pathways	pathway	NNS
408	413	which	which	WDT
414	417	may	may	MD
418	420	be	be	VB
421	429	affected	affect	VBN
430	432	in	in	IN
433	434	T	t	NN
435	440	cells	cell	NNS
441	443	by	by	IN
444	456	constitutive	constitutive	JJ
457	467	expression	expression	NN
468	470	of	of	IN
471	474	the	the	DT
475	478	nef	nef	NN
479	483	gene	gene	NN
484	488	were	be	VBD
489	497	examined	examine	VBN
497	498	.	.	.

499	510	Stimulation	stimulation	NN
511	513	of	of	IN
514	515	T	t	NN
516	521	cells	cell	NNS
522	526	with	with	IN
527	532	tumor	tumor	NN
533	541	necrosis	necrosis	NN
542	548	factor	factor	NN
548	549	,	,	,
550	563	interleukin-1	interleukin-1	NN
563	564	,	,	,
565	567	or	or	CC
568	586	lipopolysaccharide	lipopolysaccharide	NN
587	595	resulted	result	VBD
596	598	in	in	IN
599	602	the	the	DT
603	614	recruitment	recruitment	NN
615	617	of	of	IN
618	633	transcriptional	transcriptional	JJ
634	641	factors	factor	NNS
642	644	to	to	TO
645	646	a	a	DT
647	654	similar	similar	JJ
655	660	level	level	NN
661	668	whether	whether	IN
669	671	or	or	CC
672	675	not	not	RB
676	679	the	the	DT
680	685	cells	cell	NNS
686	695	expressed	express	VBD
696	699	the	the	DT
700	703	nef	nef	NN
704	708	gene	gene	NN
708	709	.	.	.

710	712	On	on	IN
713	716	the	the	DT
717	722	other	other	JJ
723	727	hand	hand	NN
727	728	,	,	,
729	740	stimulation	stimulation	NN
741	743	of	of	IN
744	745	T	t	NN
746	751	cells	cell	NNS
752	754	by	by	IN
755	763	mitogens	mitogen	NNS
764	766	or	or	CC
767	777	antibodies	antibody	NNS
778	780	to	to	TO
781	784	the	the	DT
785	791	T-cell	t-cell	NN
792	800	receptor	receptor	NN
801	802	(	(	(
802	810	TCR)-CD3	tcr)-cd3	NN
811	818	complex	complex	NN
819	827	resulted	result	VBD
828	830	in	in	IN
831	834	the	the	DT
835	850	down-regulation	down-regulation	NN
851	853	of	of	IN
854	869	transcriptional	transcriptional	JJ
870	877	factors	factor	NNS
878	886	NF-kappa	NF-kappa	NNP
887	888	B	B	NNP
889	892	and	and	CC
893	897	AP-1	ap-1	NN
898	900	in	in	IN
901	906	cells	cell	NNS
907	917	expressing	express	VBG
918	921	the	the	DT
922	925	nef	nef	NN
926	930	gene	gene	NN
931	939	compared	compare	VBN
940	944	with	with	IN
945	950	cells	cell	NNS
951	954	not	not	RB
955	965	expressing	express	VBG
966	969	the	the	DT
970	973	nef	nef	NN
974	978	gene	gene	NN
978	979	.	.	.

980	987	Because	because	IN
988	991	the	the	DT
992	995	Nef	Nef	NNP
996	1003	protein	protein	NN
1004	1008	does	do	VBZ
1009	1012	not	not	RB
1013	1019	affect	affect	VB
1020	1023	the	the	DT
1024	1031	surface	surface	NN
1032	1042	expression	expression	NN
1043	1045	of	of	IN
1046	1049	the	the	DT
1050	1057	CD3-TCR	CD3-TCR	NNP
1058	1065	complex	complex	NN
1065	1066	,	,	,
1067	1069	we	we	PRP
1070	1078	conclude	conclude	VBP
1079	1083	that	that	IN
1084	1087	the	the	DT
1088	1091	Nef	Nef	NNP
1092	1099	protein	protein	NN
1100	1114	down-regulates	down-regulate	VBZ
1115	1118	the	the	DT
1119	1134	transcriptional	transcriptional	JJ
1135	1142	factors	factor	NNS
1143	1151	NF-kappa	NF-kappa	NNP
1152	1153	B	B	NNP
1154	1157	and	and	CC
1158	1162	AP-1	ap-1	NN
1163	1165	in	in	IN
1166	1167	T	t	NN
1168	1173	cells	cell	NNS
1174	1176	in	in	FW
1177	1182	vitro	vitro	FW
1183	1190	through	through	IN
1191	1193	an	an	DT
1194	1200	effect	effect	NN
1201	1203	on	on	IN
1204	1207	the	the	DT
1208	1221	TCR-dependent	tcr-dependent	JJ
1222	1228	signal	signal	NN
1229	1241	transduction	transduction	NN
1242	1249	pathway	pathway	NN
1249	1250	.	.	.