0 5 Genes gene NNS 6 14 encoding encode VBG 15 22 general general JJ 23 33 initiation initiation NN 34 41 factors factor NNS 42 45 for for IN 46 49 RNA RNA NNP 50 60 polymerase polymerase NN 61 63 II ii CD 64 77 transcription transcription NN 78 81 are be VBP 82 91 dispersed disperse VBN 92 94 in in IN 95 98 the the DT 99 104 human human JJ 105 111 genome genome NN 111 112 . . . 114 121 General general JJ 122 135 transcription transcription NN 136 143 factors factor NNS 144 147 are be VBP 148 156 required require VBN 157 160 for for IN 161 169 accurate accurate JJ 170 180 initiation initiation NN 181 183 of of IN 184 197 transcription transcription NN 198 200 by by IN 201 204 RNA RNA NNP 205 215 polymerase polymerase NN 216 218 II ii CD 218 219 . . . 220 225 Human human JJ 226 231 cDNAs cdna NNS 232 240 encoding encode VBG 241 249 subunits subunit NNS 250 252 of of IN 253 258 these these DT 259 266 factors factor NNS 267 271 have have VBP 272 276 been be VBN 277 283 cloned clone VBN 284 287 and and CC 288 297 sequenced sequence VBN 297 298 . . . 299 304 Using use VBG 305 317 fluorescence fluorescence NN 318 320 in in FW 321 325 situ situ FW 326 339 hybridization hybridization NN 340 341 ( ( ( 341 345 FISH fish NN 345 346 ) ) ) 346 347 , , , 348 350 we we PRP 351 355 show show VBP 356 360 here here RB 361 365 that that IN 366 369 the the DT 370 375 genes gene NNS 376 384 encoding encode VBG 385 388 the the DT 389 397 TATA-box tata-box NN 398 405 binding binding NN 406 413 protein protein NN 414 415 ( ( ( 415 418 TBP TBP NNP 418 419 ) ) ) 419 420 , , , 421 426 TFIIB TFIIB NNP 426 427 , , , 428 433 TFIIE TFIIE NNP 434 439 alpha alpha NN 439 440 , , , 441 446 TFIIE TFIIE NNP 447 451 beta beta NN 451 452 , , , 453 458 RAP30 RAP30 NNP 458 459 , , , 460 465 RAP74 rap74 NN 466 469 and and CC 470 473 the the DT 474 476 62 62 CD 477 480 kDa kda NN 481 488 subunit subunit NN 488 489 , , , 490 492 of of IN 493 498 TFIIH tfiih NN 499 502 are be VBP 503 510 located located JJ 511 513 at at IN 514 517 the the DT 518 523 human human JJ 524 535 chromosomal chromosomal JJ 536 541 bands band NNS 542 549 6q26-27 6q26-27 NN 549 550 , , , 551 558 1p21-22 1p21-22 NN 558 559 , , , 560 567 3q21-24 3q21-24 NN 567 568 , , , 569 573 8p12 8p12 NN 573 574 , , , 575 580 13q14 13q14 NN 580 581 , , , 582 589 19p13.3 19p13.3 NN 590 593 and and CC 594 604 11p14-15.1 11p14-15.1 NN 604 605 , , , 606 618 respectively respectively RB 618 619 . . . 620 624 This this DT 625 634 dispersed dispersed JJ 635 647 localization localization NN 648 650 of of IN 651 652 a a DT 653 658 group group NN 659 661 of of IN 662 674 functionally functionally RB 675 682 related related JJ 683 687 gene gene NN 688 696 provides provide VBZ 697 705 insights insight NNS 706 710 into into IN 711 714 the the DT 715 724 molecular molecular JJ 725 734 mechanism mechanism NN 735 737 of of IN 738 743 human human JJ 744 750 genome genome NN 751 760 evolution evolution NN 761 764 and and CC 765 770 their their PRP$ 771 779 possible possible JJ 780 791 involvement involvement NN 792 794 in in IN 795 800 human human JJ 801 809 diseases disease NNS 809 810 . . .