0 8 Function function NN 9 11 of of IN 12 20 NF-kappa NF-kappa NNP 21 26 B/Rel B/Rel NNP 27 34 binding binding NN 35 40 sites site NNS 41 43 in in IN 44 47 the the DT 48 53 major major JJ 54 72 histocompatibility histocompatibility NN 73 80 complex complex NN 81 86 class class NN 87 89 II ii CD 90 99 invariant invariant JJ 100 105 chain chain NN 106 114 promoter promoter NN 115 117 is be VBZ 118 127 dependent dependent JJ 128 130 on on IN 131 144 cell-specific cell-specific JJ 145 152 binding binding NN 153 155 of of IN 156 165 different different JJ 166 174 NF-kappa NF-kappa NNP 175 180 B/Rel B/Rel NNP 181 189 subunits subunit NNS 189 190 . . . 192 195 The the DT 196 204 promoter promoter NN 205 207 of of IN 208 211 the the DT 212 217 human human JJ 218 223 major major JJ 224 242 histocompatibility histocompatibility NN 243 250 complex complex NN 251 256 class class NN 257 270 II-associated ii-associated JJ 271 286 invariant-chain invariant-chain JJ 287 291 gene gene NN 292 293 ( ( ( 293 295 Ii Ii NNP 295 296 ) ) ) 297 305 contains contain VBZ 306 309 two two CD 310 318 NF-kappa NF-kappa NNP 319 324 B/Rel B/Rel NNP 325 332 binding binding NN 333 338 sites site NNS 339 346 located located JJ 347 349 at at IN 350 354 -109 -109 CD 355 357 to to TO 358 362 -118 -118 CD 363 364 ( ( ( 364 366 Ii ii NN 367 372 kappa kappa NN 373 376 B-1 b-1 NN 376 377 ) ) ) 378 381 and and CC 382 386 -163 -163 CD 387 389 to to TO 390 394 -172 -172 CD 395 396 ( ( ( 396 398 Ii Ii NNP 399 404 kappa kappa NN 405 408 B-2 b-2 NN 408 409 ) ) ) 410 414 from from IN 415 418 the the DT 419 432 transcription transcription NN 433 438 start start NN 439 443 site site NN 443 444 . . . 445 447 We we PRP 448 454 report report VBP 455 459 here here RB 460 464 that that IN 465 468 the the DT 469 481 differential differential JJ 482 490 function function NN 491 493 of of IN 494 498 each each DT 499 501 of of IN 502 507 these these DT 508 516 NF-kappa NF-kappa NNP 517 522 B/Rel B/Rel NNP 523 528 sites site NNS 529 531 in in IN 532 539 several several JJ 540 548 distinct distinct JJ 549 553 cell cell NN 554 559 types type NNS 560 567 depends depend VBZ 568 570 on on IN 571 584 cell-specific cell-specific JJ 585 592 binding binding NN 593 595 of of IN 596 604 NF-kappa NF-kappa NNP 605 610 B/Rel B/Rel NNP 611 624 transcription transcription NN 625 632 factors factor NNS 632 633 . . . 634 636 Ii Ii NNP 637 642 kappa kappa NN 643 646 B-1 b-1 NN 647 649 is be VBZ 650 651 a a DT 652 660 positive positive JJ 661 671 regulatory regulatory JJ 672 679 element element NN 680 682 in in IN 683 689 B-cell b-cell NN 690 695 lines line NNS 696 699 and and CC 700 702 in in IN 703 706 the the DT 707 720 Ii-expressing ii-expressing JJ 721 727 T-cell t-cell NN 728 732 line line NN 732 733 , , , 734 736 H9 H9 NNP 736 737 , , , 738 741 but but CC 742 746 acts act VBZ 747 749 as as IN 750 751 a a DT 752 760 negative negative JJ 761 771 regulatory regulatory JJ 772 779 element element NN 780 782 in in IN 783 797 myelomonocytic myelomonocytic JJ 798 801 and and CC 802 806 glia glia NN 807 811 cell cell NN 812 817 lines line NNS 817 818 . . . 819 821 In in FW 822 826 vivo vivo FW 827 838 protein-DNA protein-dna JJ 839 847 contacts contact NNS 848 851 are be VBP 852 862 detectable detectable JJ 863 865 at at IN 866 868 Ii Ii NNP 869 874 kappa kappa NN 875 878 B-1 b-1 NN 879 881 in in IN 882 886 cell cell NN 887 892 lines line NNS 893 895 in in IN 896 901 which which WDT 902 906 this this DT 907 911 site site NN 912 914 is be VBZ 915 925 functional functional JJ 926 928 as as IN 929 935 either either CC 936 937 a a DT 938 946 positive positive JJ 947 949 or or CC 950 958 negative negative JJ 959 968 regulator regulator NN 968 969 . . . 970 985 Electrophoretic electrophoretic JJ 986 994 mobility mobility NN 995 1005 supershift supershift NN 1006 1012 assays assay NNS 1013 1022 determine determine VBP 1023 1027 that that IN 1028 1035 members member NNS 1036 1038 of of IN 1039 1042 the the DT 1043 1051 NF-kappa NF-kappa NNP 1052 1057 B/Rel B/Rel NNP 1058 1064 family family NN 1065 1067 of of IN 1068 1081 transcription transcription NN 1082 1089 factors factor NNS 1090 1093 can can MD 1094 1098 bind bind VB 1099 1101 to to TO 1102 1106 this this DT 1107 1111 site site NN 1112 1114 in in FW 1115 1120 vitro vitro FW 1121 1124 and and CC 1125 1129 that that IN 1130 1141 DNA-binding dna-binding JJ 1142 1151 complexes complex NNS 1152 1156 that that WDT 1157 1164 contain contain VBP 1165 1168 p50 p50 NN 1168 1169 , , , 1170 1173 p52 p52 NN 1173 1174 , , , 1175 1178 p65 p65 NN 1178 1179 , , , 1180 1183 and and CC 1184 1188 cRel crel NN 1189 1198 correlate correlate VBP 1199 1203 with with IN 1204 1212 positive positive JJ 1213 1223 regulation regulation NN 1224 1231 whereas whereas IN 1232 1235 the the DT 1236 1244 presence presence NN 1245 1247 of of IN 1248 1251 p50 p50 NN 1252 1262 correlates correlate VBZ 1263 1267 with with IN 1268 1276 negative negative JJ 1277 1287 regulation regulation NN 1287 1288 . . . 1289 1291 Ii Ii NNP 1292 1297 kappa kappa NN 1298 1301 B-2 B-2 NNP 1302 1304 is be VBZ 1305 1306 a a DT 1307 1311 site site NN 1312 1314 of of IN 1315 1323 positive positive JJ 1324 1334 regulation regulation NN 1335 1337 in in IN 1338 1344 B-cell b-cell NN 1345 1350 lines line NNS 1351 1354 and and CC 1355 1356 a a DT 1357 1361 site site NN 1362 1364 of of IN 1365 1373 negative negative JJ 1374 1384 regulation regulation NN 1385 1387 in in IN 1388 1390 H9 H9 NNP 1391 1392 T t NN 1393 1398 cells cell NNS 1398 1399 , , , 1400 1414 myelomonocytic myelomonocytic JJ 1414 1415 , , , 1416 1419 and and CC 1420 1425 glial glial JJ 1426 1430 cell cell NN 1431 1436 lines line NNS 1436 1437 . . . 1438 1440 In in FW 1441 1445 vivo vivo FW 1446 1455 occupancy occupancy NN 1456 1458 of of IN 1459 1463 this this DT 1464 1468 site site NN 1469 1471 is be VBZ 1472 1480 observed observe VBN 1481 1485 only only RB 1486 1488 in in IN 1489 1492 the the DT 1493 1495 H9 H9 NNP 1496 1502 T-cell T-cell NNP 1503 1507 line line NN 1507 1508 . . . 1509 1514 Again again RB 1514 1515 , , , 1516 1518 in in FW 1519 1524 vitro vitro FW 1525 1535 supershift supershift NN 1536 1543 studies study NNS 1544 1552 indicate indicate VBP 1553 1557 that that IN 1558 1561 the the DT 1562 1570 presence presence NN 1571 1573 of of IN 1574 1577 p50 p50 NN 1577 1578 , , , 1579 1582 p52 p52 NN 1582 1583 , , , 1584 1587 p65 p65 NN 1587 1588 , , , 1589 1592 and and CC 1593 1597 cRel crel NN 1598 1608 correlates correlate VBZ 1609 1613 with with IN 1614 1622 positive positive JJ 1623 1631 function function NN 1632 1639 whereas whereas IN 1640 1643 the the DT 1644 1652 presence presence NN 1653 1655 of of IN 1656 1660 only only JJ 1661 1664 p50 p50 NN 1665 1668 and and CC 1669 1672 p52 p52 NN 1673 1683 correlates correlate VBZ 1684 1688 with with IN 1689 1697 negative negative JJ 1698 1706 function function NN 1706 1707 . . . 1708 1712 This this DT 1713 1725 differential differential JJ 1726 1733 binding binding NN 1734 1736 of of IN 1737 1745 specific specific JJ 1746 1754 NF-kappa NF-kappa NNP 1755 1760 B/Rel B/Rel NNP 1761 1769 subunits subunit NNS 1770 1772 is be VBZ 1773 1779 likely likely JJ 1780 1782 to to TO 1783 1790 mediate mediate VB 1791 1794 the the DT 1795 1804 disparate disparate JJ 1805 1814 functions function NNS 1815 1817 of of IN 1818 1823 these these DT 1824 1827 two two CD 1828 1836 NF-kappa NF-kappa NNP 1837 1842 B/Rel B/Rel NNP 1843 1850 binding binding NN 1851 1856 sites site NNS 1856 1857 . . .