0 8 Patterns pattern NNS 9 11 of of IN 12 15 Pan Pan NNP 16 26 expression expression NN 27 30 and and CC 31 35 role role NN 36 38 of of IN 39 42 Pan Pan NNP 43 51 proteins protein NNS 52 54 in in IN 55 64 endocrine endocrine JJ 65 69 cell cell NN 70 83 type-specific type-specific JJ 84 91 complex complex NN 92 101 formation formation NN 101 102 . . . 104 107 The the DT 108 111 Pan Pan NNP 112 116 gene gene NN 117 124 encodes encode VBZ 125 127 at at IN 128 133 least least JJS 134 137 two two CD 138 146 distinct distinct JJ 147 158 transcripts transcript NNS 158 159 , , , 160 165 Pan-1 pan-1 NN 166 169 and and CC 170 175 Pan-2 pan-2 NN 176 177 ( ( ( 177 181 also also RB 182 187 known know VBN 188 190 as as IN 191 194 E47 E47 NNP 195 198 and and CC 199 202 E12 E12 NNP 202 203 , , , 204 216 respectively respectively RB 216 217 ) ) ) 217 218 , , , 219 221 by by IN 222 225 the the DT 226 235 mechanism mechanism NN 236 238 of of IN 239 250 alternative alternative JJ 251 254 RNA RNA NNP 255 263 splicing splicing NN 263 264 . . . 265 273 Northern Northern NNP 274 278 blot blot NN 279 287 analyses analysis NNS 288 297 performed perform VBN 298 300 on on IN 301 304 rat rat NN 305 308 and and CC 309 314 mouse mouse NN 315 322 tissues tissue NNS 323 327 have have VBP 328 336 detected detect VBN 337 349 ubiquitously ubiquitously RB 350 359 expressed express VBN 360 363 Pan Pan NNP 364 375 transcripts transcript NNS 375 376 , , , 377 380 but but CC 381 384 the the DT 385 394 abundance abundance NN 394 395 , , , 396 408 distribution distribution NN 408 409 , , , 410 413 and and CC 414 418 form form NN 419 421 of of IN 422 425 Pan Pan NNP 426 434 proteins protein NNS 435 439 have have VBP 440 443 not not RB 444 448 been be VBN 449 456 clearly clearly RB 457 464 defined define VBN 464 465 . . . 466 473 Studies study NNS 474 476 of of IN 477 481 cell cell NN 482 487 lines line NNS 488 500 representing represent VBG 501 510 endocrine endocrine NN 510 511 , , , 512 522 fibroblast fibroblast NN 522 523 , , , 524 527 and and CC 528 536 lymphoid lymphoid JJ 537 545 lineages lineage NNS 546 551 using use VBG 552 562 polyclonal polyclonal JJ 563 571 antisera antiserum NNS 572 574 to to TO 575 581 detect detect VB 582 585 E2A E2A NNP 586 594 proteins protein NNS 595 599 have have VBP 600 609 suggested suggest VBN 610 614 that that IN 615 626 significant significant JJ 627 630 E2A E2A NNP 631 638 protein protein NN 639 649 expression expression NN 650 652 is be VBZ 653 663 restricted restrict VBN 664 666 to to TO 667 680 B-lymphocytes b-lymphocyte NNS 680 681 . . . 682 684 We we PRP 685 689 have have VBP 690 699 developed develop VBN 700 701 a a DT 702 712 monoclonal monoclonal JJ 713 721 antibody antibody NN 721 722 , , , 723 726 Yae Yae NNP 726 727 , , , 728 733 which which WDT 734 736 is be VBZ 737 745 specific specific JJ 746 749 for for IN 750 757 Pan/E2A Pan/E2A NNP 758 766 proteins protein NNS 766 767 , , , 768 771 and and CC 772 776 have have VBP 777 781 used use VBN 782 785 the the DT 786 789 Yae yae NN 790 798 antibody antibody NN 799 801 to to TO 802 809 examine examine VB 810 811 a a DT 812 819 variety variety NN 820 822 of of IN 823 832 endocrine endocrine JJ 833 836 and and CC 837 849 nonendocrine nonendocrine JJ 850 854 cell cell NN 855 863 lineages lineage NNS 864 867 for for IN 868 879 differences difference NNS 880 882 in in IN 883 890 Pan/E2A Pan/E2A NNP 891 898 protein protein NN 899 909 expression expression NN 909 910 , , , 911 922 subcellular subcellular JJ 923 935 localization localization NN 935 936 , , , 937 940 and and CC 941 952 heteromeric heteromeric JJ 953 960 complex complex NN 961 970 formation formation NN 970 971 . . . 972 974 In in IN 975 983 contrast contrast NN 984 986 to to TO 987 995 previous previous JJ 996 1003 results result NNS 1004 1012 obtained obtain VBN 1013 1018 using use VBG 1019 1029 polyclonal polyclonal JJ 1030 1039 antiseras antisera NNS 1040 1042 to to TO 1043 1049 detect detect VB 1050 1057 Pan/E2A Pan/E2A NNP 1058 1066 proteins protein NNS 1066 1067 , , , 1068 1070 we we PRP 1071 1077 report report VBP 1078 1088 comparable comparable JJ 1089 1095 levels level NNS 1096 1098 of of IN 1099 1102 Pan Pan NNP 1103 1111 proteins protein NNS 1112 1114 in in IN 1115 1122 GH/PRL- gh/prl- NN 1123 1126 and and CC 1127 1144 insulin-producing insulin-producing JJ 1144 1145 , , , 1146 1148 B- b- NN 1149 1152 and and CC 1153 1165 T-lymphocyte t-lymphocyte NN 1166 1171 cells cell NNS 1171 1172 . . . 1173 1178 IEF-1 IEF-1 NNP 1178 1179 , , , 1180 1181 a a DT 1182 1192 pancreatic pancreatic JJ 1193 1202 beta-cell beta-cell NN 1203 1216 type-specific type-specific JJ 1217 1224 complex complex NN 1225 1233 believed believe VBN 1234 1236 to to TO 1237 1245 regulate regulate VB 1246 1253 insulin insulin NN 1254 1264 expression expression NN 1264 1265 , , , 1266 1268 is be VBZ 1269 1281 demonstrated demonstrate VBN 1282 1284 to to TO 1285 1292 consist consist VB 1293 1295 of of IN 1296 1298 at at IN 1299 1304 least least JJS 1305 1308 two two CD 1309 1317 distinct distinct JJ 1318 1325 species specie NNS 1325 1326 , , , 1327 1330 one one CD 1331 1333 of of IN 1334 1339 which which WDT 1340 1344 does do VBZ 1345 1348 not not RB 1349 1356 contain contain VB 1357 1360 Pan Pan NNP 1361 1370 molecules molecule NNS 1370 1371 . . . 1372 1380 Although although IN 1381 1383 it it PRP 1384 1387 has have VBZ 1388 1392 been be VBN 1393 1403 postulated postulate VBN 1404 1408 that that IN 1409 1418 pituitary pituitary JJ 1419 1428 endocrine endocrine JJ 1429 1434 cells cell NNS 1435 1438 and and CC 1439 1449 pancreatic pancreatic JJ 1450 1459 endocrine endocrine JJ 1460 1470 beta-cells beta-cell NNS 1471 1476 share share VBP 1477 1486 identical identical JJ 1487 1494 Pan/E2A Pan/E2A NNP 1495 1504 complexes complex NNS 1504 1505 , , , 1506 1520 native-Western native-western JJ 1521 1529 analyses analysis NNS 1530 1532 of of IN 1533 1542 pituitary pituitary JJ 1543 1546 and and CC 1547 1556 endocrine endocrine JJ 1557 1567 beta-cells beta-cell NNS 1568 1574 detect detect VBP 1575 1578 Pan Pan NNP 1579 1587 proteins protein NNS 1588 1590 in in IN 1591 1599 distinct distinct JJ 1600 1604 cell cell NN 1605 1618 type-specific type-specific JJ 1619 1628 complexes complex NNS 1628 1629 . . .