0 10 Functional functional JJ 11 16 block block NN 17 20 for for IN 21 22 1 1 CD 23 48 alpha,25-dihydroxyvitamin alpha,25-dihydroxyvitamin NN 49 60 D3-mediated d3-mediated JJ 61 65 gene gene NN 66 76 regulation regulation NN 77 79 in in IN 80 85 human human JJ 86 87 B B NNP 88 99 lymphocytes lymphocyte NNS 99 100 . . . 102 110 Elements element NNS 111 120 necessary necessary JJ 121 124 for for IN 125 128 the the DT 129 136 steroid steroid NN 137 144 hormone hormone NN 145 146 1 1 CD 147 172 alpha,25-dihydroxyvitamin alpha,25-dihydroxyvitamin NN 173 175 D3 d3 NN 176 177 ( ( ( 177 178 1 1 CD 179 196 alpha,25-(OH)2D3) alpha,25-(oh)2d3) NN 197 199 to to TO 200 206 induce induce VB 207 208 a a DT 209 219 biological biological JJ 220 228 response response NN 229 236 include include VBP 237 240 the the DT 241 249 presence presence NN 250 252 of of IN 253 261 specific specific JJ 262 275 intracellular intracellular JJ 276 285 receptors receptor NNS 286 287 ( ( ( 287 294 vitamin vitamin NN 295 297 D3 d3 NN 298 307 receptors receptor NNS 308 309 ( ( ( 309 312 VDR VDR NNP 312 313 ) ) ) 313 314 ) ) ) 315 318 and and CC 319 329 modulation modulation NN 330 332 of of IN 333 337 gene gene NN 338 348 expression expression NN 349 352 via via IN 353 370 hormone-activated hormone-activated JJ 371 379 receptor receptor NN 380 387 binding bind VBG 388 390 to to TO 391 401 regulatory regulatory JJ 402 409 regions region NNS 410 412 of of IN 413 419 target target NN 420 425 genes gene NNS 425 426 . . . 427 432 These these DT 433 443 parameters parameter NNS 444 448 were be VBD 449 457 examined examine VBN 458 460 in in IN 461 467 normal normal JJ 468 471 and and CC 472 484 Epstein-Barr epstein-barr JJ 485 503 virus-immortalized virus-immortalized JJ 504 509 human human JJ 510 511 B B NNP 512 517 cells cell NNS 518 521 and and CC 522 530 compared compare VBN 531 535 with with IN 536 537 1 1 CD 538 565 alpha,25-(OH)2D3-responsive alpha,25-(oh)2d3-responsive JJ 566 571 cells cell NNS 572 574 of of IN 575 578 the the DT 579 580 T t NN 581 584 and and CC 585 594 monocytic monocytic JJ 595 603 lineages lineage NNS 603 604 . . . 605 613 Although although IN 614 621 resting rest VBG 622 631 tonsillar tonsillar JJ 632 633 B B NNP 634 639 cells cell NNS 640 643 did do VBD 644 647 not not RB 648 655 express express VB 656 659 VDR VDR NNP 660 664 mRNA mRNA NNP 664 665 , , , 666 676 activation activation NN 677 679 of of IN 680 685 these these DT 686 691 cells cell NNS 692 696 with with IN 697 710 interleukin-4 interleukin-4 NN 711 718 induced induce VBD 719 722 VDR VDR NNP 723 725 in in IN 726 729 the the DT 730 737 absence absence NN 738 740 of of IN 741 752 exogenously exogenously RB 753 765 supplemented supplement VBN 766 767 1 1 CD 768 784 alpha,25-(OH)2D3 alpha,25-(oh)2d3 NN 784 785 . . . 786 788 As as IN 789 799 indicators indicator NNS 800 802 of of IN 803 819 hormone-mediated hormone-mediated JJ 820 824 gene gene NN 825 835 regulation regulation NN 836 838 we we PRP 839 847 analyzed analyze VBD 848 858 modulation modulation NN 859 861 of of IN 862 866 CD23 CD23 NNP 866 867 , , , 868 869 a a DT 870 876 common common JJ 877 878 B B NNP 879 892 cell/monocyte cell/monocyte NN 893 900 surface surface NN 901 908 antigen antigen NN 908 909 , , , 910 913 and and CC 914 928 24-hydroxylase 24-hydroxylase NN 928 929 . . . 930 931 1 1 CD 932 948 alpha,25-(OH)2D3 alpha,25-(oh)2d3 NN 949 958 inhibited inhibit VBD 959 963 CD23 cd23 NN 964 974 expression expression NN 975 977 in in IN 978 982 U937 u937 NN 983 988 cells cell NNS 988 989 , , , 990 993 yet yet RB 994 1000 failed fail VBD 1001 1003 to to TO 1004 1012 modulate modulate VB 1013 1017 CD23 CD23 NNP 1018 1028 expression expression NN 1029 1031 in in IN 1032 1033 B B NNP 1034 1039 cells cell NNS 1039 1040 . . . 1041 1052 Furthermore furthermore RB 1052 1053 , , , 1054 1055 1 1 CD 1056 1072 alpha,25-(OH)2D3 alpha,25-(oh)2d3 NN 1073 1080 induced induce VBD 1081 1095 24-hydroxylase 24-hydroxylase NN 1096 1100 mRNA mrna NN 1101 1111 expression expression NN 1112 1115 and and CC 1116 1125 metabolic metabolic JJ 1126 1134 activity activity NN 1135 1137 in in IN 1138 1142 both both DT 1143 1147 U937 u937 NN 1148 1153 cells cell NNS 1154 1157 and and CC 1158 1174 lectin-activated lectin-activated JJ 1175 1176 T t NN 1177 1182 cells cell NNS 1182 1183 , , , 1184 1187 yet yet RB 1188 1194 failed fail VBD 1195 1197 to to TO 1198 1204 induce induce VB 1205 1219 24-hydroxylase 24-hydroxylase NN 1220 1224 mRNA mRNA NNP 1225 1227 or or CC 1228 1231 its its PRP$ 1232 1241 metabolic metabolic JJ 1242 1250 activity activity NN 1251 1253 in in IN 1254 1255 B B NNP 1256 1261 cells cell NNS 1261 1262 . . . 1263 1268 These these DT 1269 1277 findings finding NNS 1278 1285 suggest suggest VBP 1286 1290 that that IN 1291 1299 although although IN 1300 1305 human human JJ 1306 1307 B B NNP 1308 1319 lymphocytes lymphocyte NNS 1320 1323 can can MD 1324 1331 express express VB 1332 1335 VDR VDR NNP 1336 1340 mRNA mRNA NNP 1341 1344 and and CC 1345 1352 protein protein NN 1352 1353 , , , 1354 1358 they they PRP 1359 1366 exhibit exhibit VBP 1367 1368 a a DT 1369 1379 functional functional JJ 1380 1385 block block NN 1386 1389 for for IN 1390 1397 vitamin vitamin NN 1398 1409 D-dependent d-dependent JJ 1410 1414 gene gene NN 1415 1425 regulation regulation NN 1425 1426 . . .