0 11 Alternative alternative JJ 12 20 splicing splicing NN 21 23 of of IN 24 27 RNA RNA NNP 28 39 transcripts transcript NNS 40 47 encoded encode VBN 48 50 by by IN 51 54 the the DT 55 61 murine murine JJ 62 66 p105 p105 NN 67 75 NF-kappa NF-kappa NNP 76 77 B B NNP 78 82 gene gene NN 83 92 generates generate VBZ 93 94 I i NN 95 100 kappa kappa NN 101 102 B B NNP 103 108 gamma gamma NN 109 117 isoforms isoform NNS 118 122 with with IN 123 132 different different JJ 133 143 inhibitory inhibitory JJ 144 154 activities activity NNS 154 155 . . . 157 160 The the DT 161 165 gene gene NN 166 174 encoding encode VBG 175 178 the the DT 179 186 105-kDa 105-kda JJ 187 194 protein protein NN 195 196 ( ( ( 196 200 p105 p105 NN 200 201 ) ) ) 202 211 precursor precursor NN 212 214 of of IN 215 218 the the DT 219 222 p50 p50 NN 223 230 subunit subunit NN 231 233 of of IN 234 247 transcription transcription NN 248 254 factor factor NN 255 263 NF-kappa NF-kappa NNP 264 265 B B NNP 266 270 also also RB 271 278 encodes encode VBZ 279 280 a a DT 281 284 p70 p70 NN 285 286 I i NN 287 292 kappa kappa NN 293 294 B B NNP 295 302 protein protein NN 302 303 , , , 304 305 I i NN 306 311 kappa kappa NN 312 313 B B NNP 314 319 gamma gamma NN 319 320 , , , 321 326 which which WDT 327 329 is be VBZ 330 339 identical identical JJ 340 342 to to TO 343 346 the the DT 347 357 C-terminal c-terminal JJ 358 361 607 607 CD 362 367 amino amino NN 368 373 acids acid NNS 374 376 of of IN 377 381 p105 p105 NN 381 382 . . . 383 387 Here here RB 388 390 we we PRP 391 395 show show VBP 396 400 that that IN 401 412 alternative alternative JJ 413 416 RNA RNA NNP 417 425 splicing splicing NN 426 435 generates generate VBZ 436 437 I i NN 438 443 kappa kappa NN 444 445 B B NNP 446 451 gamma gamma NN 452 460 isoforms isoform NNS 461 465 with with IN 466 476 properties property NNS 477 486 different different JJ 487 491 from from IN 492 497 those those DT 498 500 of of IN 501 504 p70 p70 NN 504 505 . . . 506 509 One one CD 510 516 63-kDa 63-kda JJ 517 524 isoform isoform NN 524 525 , , , 526 532 termed term VBN 533 534 I i NN 535 540 kappa kappa NN 541 542 B B NNP 543 550 gamma-1 gamma-1 NN 550 551 , , , 552 557 which which WDT 558 563 lacks lack VBZ 564 566 59 59 CD 567 572 amino amino NN 573 578 acids acid NNS 579 589 C-terminal c-terminal JJ 590 592 to to TO 593 600 ankyrin ankyrin NN 601 607 repeat repeat NN 608 609 7 7 CD 609 610 , , , 611 614 has have VBZ 615 616 a a DT 617 622 novel novel JJ 623 631 35-amino 35-amino NN 632 636 acid acid NN 637 638 C C NNP 639 647 terminus terminus NNP 648 655 encoded encode VBN 656 658 by by IN 659 661 an an DT 662 673 alternative alternative JJ 674 681 reading reading NN 682 687 frame frame NN 688 690 of of IN 691 694 the the DT 695 699 p105 p105 NN 700 704 gene gene NN 704 705 . . . 706 707 A a DT 708 714 55-kDa 55-kda JJ 715 722 isoform isoform NN 722 723 , , , 724 725 I i NN 726 731 kappa kappa NN 732 733 B B NNP 734 741 gamma-2 gamma-2 NN 741 742 , , , 743 748 lacks lack VBZ 749 752 the the DT 753 756 190 190 CD 757 767 C-terminal c-terminal JJ 768 773 amino amino NN 774 779 acids acid NNS 780 782 of of IN 783 787 p70I p70i NN 788 793 kappa kappa NN 794 795 B B NNP 796 801 gamma gamma NN 801 802 . . . 803 805 In in IN 806 814 contrast contrast NN 815 817 to to TO 818 822 p70I p70i NN 823 828 kappa kappa NN 829 830 B B NNP 831 836 gamma gamma NN 836 837 , , , 838 843 which which WDT 844 846 is be VBZ 847 848 a a DT 849 860 cytoplasmic cytoplasmic JJ 861 868 protein protein NN 868 869 , , , 870 871 I i NN 872 877 kappa kappa NN 878 879 B B NNP 880 887 gamma-1 gamma-1 NN 888 890 is be VBZ 891 896 found find VBN 897 899 in in IN 900 904 both both CC 905 908 the the DT 909 918 cytoplasm cytoplasm NN 919 922 and and CC 923 930 nucleus nucleus NN 930 931 , , , 932 939 whereas whereas IN 940 941 I i NN 942 947 kappa kappa NN 948 949 B B NNP 950 957 gamma-2 gamma-2 NN 958 960 is be VBZ 961 974 predominantly predominantly RB 975 982 nuclear nuclear JJ 982 983 . . . 984 987 The the DT 988 989 I i NN 990 995 kappa kappa NN 996 997 B B NNP 998 1003 gamma gamma NN 1004 1012 isoforms isoform NNS 1013 1017 also also RB 1018 1025 display display VBP 1026 1037 differences difference NNS 1038 1040 in in IN 1041 1052 specificity specificity NN 1053 1056 and and CC 1057 1065 affinity affinity NN 1066 1069 for for IN 1070 1082 Rel/NF-kappa Rel/NF-kappa NNP 1083 1084 B B NNP 1085 1093 proteins protein NNS 1093 1094 . . . 1095 1100 While while IN 1101 1105 p70I p70i NN 1106 1111 kappa kappa NN 1112 1113 B B NNP 1114 1119 gamma gamma NN 1120 1128 inhibits inhibit VBZ 1129 1133 p50- p50- NN 1133 1134 , , , 1135 1139 p65- p65- NN 1139 1140 , , , 1141 1144 and and CC 1145 1159 c-Rel-mediated c-rel-mediated JJ 1160 1175 transactivation transactivation NN 1176 1182 and/or and/or CC 1183 1186 DNA dna NN 1187 1194 binding binding NN 1194 1195 , , , 1196 1200 both both CC 1201 1202 I i NN 1203 1208 kappa kappa NN 1209 1210 B B NNP 1211 1218 gamma-1 gamma-1 NN 1219 1222 and and CC 1223 1224 I i NN 1225 1230 kappa kappa NN 1231 1232 B B NNP 1233 1240 gamma-2 gamma-2 NN 1241 1244 are be VBP 1245 1253 specific specific JJ 1254 1257 for for IN 1258 1261 p50 p50 NN 1262 1265 and and CC 1266 1270 have have VBP 1271 1280 different different JJ 1281 1291 affinities affinity NNS 1292 1295 for for IN 1296 1300 this this DT 1301 1308 subunit subunit NN 1308 1309 . . . 1310 1313 The the DT 1314 1321 absence absence NN 1322 1324 in in IN 1325 1326 I i NN 1327 1332 kappa kappa NN 1333 1334 B B NNP 1335 1342 gamma-1 gamma-1 NN 1343 1346 and and CC 1347 1348 I i NN 1349 1354 kappa kappa NN 1355 1356 B B NNP 1357 1364 gamma-2 gamma-2 NN 1365 1367 of of IN 1368 1369 a a DT 1370 1377 protein protein NN 1378 1384 kinase kinase NNP 1385 1386 A a NN 1387 1391 site site NN 1392 1397 whose whose WP$ 1398 1413 phosphorylation phosphorylation NN 1414 1423 modulates modulate VBZ 1424 1428 p70I p70i NN 1429 1434 kappa kappa NN 1435 1436 B B NNP 1437 1442 gamma gamma NN 1443 1453 inhibitory inhibitory JJ 1454 1462 activity activity NN 1463 1471 suggests suggest VBZ 1472 1476 that that IN 1477 1488 alternative alternative JJ 1489 1492 RNA RNA NNP 1493 1501 splicing splicing NN 1502 1505 may may MD 1506 1508 be be VB 1509 1513 used use VBN 1514 1516 to to TO 1517 1525 generate generate VB 1526 1527 I i NN 1528 1533 kappa kappa NN 1534 1535 B B NNP 1536 1541 gamma gamma NN 1542 1550 isoforms isoform NNS 1551 1555 that that WDT 1556 1563 respond respond VBP 1564 1575 differently differently RB 1576 1578 to to TO 1579 1592 intracellular intracellular JJ 1593 1600 signals signal NNS 1600 1601 . . .