0 18 Lipopolysaccharide Lipopolysaccharide NNP 19 28 induction induction NN 29 31 of of IN 32 38 tissue tissue NN 39 45 factor factor NN 46 50 gene gene NN 51 61 expression expression NN 62 64 in in IN 65 74 monocytic monocytic JJ 75 80 cells cell NNS 81 83 is be VBZ 84 92 mediated mediate VBN 93 95 by by IN 96 103 binding binding NN 104 106 of of IN 107 116 c-Rel/p65 c-rel/p65 NN 117 129 heterodimers heterodimer NNS 130 132 to to TO 133 134 a a DT 135 140 kappa kappa NN 141 147 B-like b-like JJ 148 152 site site NN 152 153 . . . 155 163 Exposure exposure NN 164 166 of of IN 167 176 monocytic monocytic JJ 177 182 cells cell NNS 183 185 to to TO 186 195 bacterial bacterial JJ 196 214 lipopolysaccharide lipopolysaccharide NN 215 216 ( ( ( 216 219 LPS LPS NNP 219 220 ) ) ) 221 230 activates activate VBZ 231 234 the the DT 235 243 NF-kappa NF-kappa NNP 244 249 B/Rel B/Rel NNP 250 256 family family NN 257 259 of of IN 260 268 proteins protein NNS 269 272 and and CC 273 278 leads lead VBZ 279 281 to to TO 282 285 the the DT 286 291 rapid rapid JJ 292 301 induction induction NN 302 304 of of IN 305 317 inflammatory inflammatory JJ 318 322 gene gene NN 323 331 products product NNS 331 332 , , , 333 342 including include VBG 343 349 tissue tissue NN 350 356 factor factor NN 357 358 ( ( ( 358 360 TF TF NNP 360 361 ) ) ) 361 362 . . . 363 365 TF TF NNP 366 368 is be VBZ 369 372 the the DT 373 380 primary primary JJ 381 389 cellular cellular JJ 390 399 initiator initiator NN 400 402 of of IN 403 406 the the DT 407 418 coagulation coagulation NN 419 427 protease protease NN 428 436 cascades cascade NNS 436 437 . . . 438 442 Here here RB 443 445 we we PRP 446 452 report report VBP 453 456 the the DT 457 473 characterization characterization NN 474 476 of of IN 477 478 a a DT 479 486 nuclear nuclear JJ 487 494 complex complex NN 495 499 from from IN 500 505 human human JJ 506 515 monocytic monocytic JJ 516 521 cells cell NNS 522 526 that that WDT 527 532 bound bind VBD 533 535 to to TO 536 537 a a DT 538 543 kappa kappa NN 544 550 B-like b-like JJ 551 555 site site NN 555 556 , , , 557 572 5’-CGGAGTTTCC-3 5'-CGGAGTTTCC-3 NNP 572 573 ’ ' '' 573 574 , , , 575 577 in in IN 578 581 the the DT 582 593 5’-flanking 5'-flanking JJ 594 600 region region NN 601 603 of of IN 604 607 the the DT 608 613 human human JJ 614 616 TF tf NN 617 621 gene gene NN 621 622 . . . 623 627 This this DT 628 635 nuclear nuclear JJ 636 643 complex complex NN 644 647 was be VBD 648 657 activated activate VBN 658 660 by by IN 661 664 LPS lps NN 665 669 with with IN 670 678 kinetics kinetic NNS 679 683 that that WDT 684 692 preceded precede VBD 693 702 induction induction NN 703 705 of of IN 706 709 the the DT 710 712 TF TF NNP 713 717 gene gene NN 717 718 . . . 719 721 In in FW 722 727 vitro vitro FW 728 735 binding binding NN 736 743 studies study NNS 744 756 demonstrated demonstrate VBD 757 761 that that IN 762 765 the the DT 766 768 TF TF NNP 769 773 site site NN 774 779 bound bind VBD 780 790 translated translate VBN 791 796 c-Rel c-rel NN 797 800 and and CC 801 804 p65 p65 NN 805 815 homodimers homodimer NNS 816 819 but but CC 820 823 not not RB 824 831 p50/p65 p50/p65 NN 832 844 heterodimers heterodimer NNS 845 847 or or CC 848 851 p50 p50 NN 852 862 homodimers homodimer NNS 862 863 . . . 864 873 Base-pair base-pair JJ 874 887 substitutions substitution NNS 888 890 in in IN 891 894 the the DT 895 897 TF TF NNP 898 902 site site NN 903 912 indicated indicate VBD 913 917 that that IN 918 921 the the DT 922 930 presence presence NN 931 933 of of IN 934 935 a a DT 936 944 cytosine cytosine NN 945 947 at at IN 948 956 position position NN 957 958 1 1 CD 959 968 precluded preclude VBD 969 976 binding binding NN 977 979 of of IN 980 988 NF-kappa NF-kappa NNP 989 991 B. B. NNP 992 994 In in IN 995 999 fact fact NN 999 1000 , , , 1001 1006 under under IN 1007 1025 low-ionic-strength low-ionic-strength JJ 1026 1036 conditions condition NNS 1036 1037 , , , 1038 1041 the the DT 1042 1044 TF TF NNP 1045 1052 complex complex NN 1053 1056 did do VBD 1057 1060 not not RB 1061 1068 migrate migrate VB 1069 1073 with with IN 1074 1084 translated translate VBN 1085 1092 p50/p65 p50/p65 NN 1093 1099 dimers dimer NNS 1100 1103 but but CC 1104 1111 instead instead RB 1112 1122 comigrated comigrate VBD 1123 1127 with with IN 1128 1137 c-Rel/p65 c-rel/p65 NN 1138 1144 dimers dimer NNS 1144 1145 . . . 1146 1156 Antibodies antibody NNS 1157 1164 against against IN 1165 1168 the the DT 1169 1177 NF-kappa NF-kappa NNP 1178 1179 B B NNP 1180 1183 and and CC 1184 1187 Rel Rel NNP 1188 1196 proteins protein NNS 1197 1200 and and CC 1201 1203 UV UV NNP 1204 1217 cross-linking cross-linking JJ 1218 1225 studies study NNS 1226 1234 revealed reveal VBD 1235 1238 the the DT 1239 1247 presence presence NN 1248 1250 of of IN 1251 1256 c-Rel c-rel NN 1257 1260 and and CC 1261 1264 p65 p65 NN 1265 1268 and and CC 1269 1272 the the DT 1273 1280 absence absence NN 1281 1283 of of IN 1284 1287 p50 p50 NN 1288 1290 in in IN 1291 1294 the the DT 1295 1297 TF TF NNP 1298 1305 complex complex NN 1306 1309 and and CC 1310 1317 further further RB 1318 1324 showed show VBD 1325 1329 that that IN 1330 1339 c-Rel/p65 c-rel/p65 NN 1340 1352 heterodimers heterodimer NNS 1353 1364 selectively selectively RB 1365 1370 bound bind VBD 1371 1373 to to TO 1374 1377 the the DT 1378 1380 TF TF NNP 1381 1386 kappa kappa NN 1387 1393 B-like b-like JJ 1394 1398 site site NN 1398 1399 . . . 1400 1410 Functional functional JJ 1411 1418 studies study NNS 1419 1428 indicated indicate VBD 1429 1433 that that IN 1434 1437 the the DT 1438 1440 TF TF NNP 1441 1445 site site NN 1446 1455 conferred confer VBD 1456 1459 LPS LPS NNP 1460 1472 inducibility inducibility NN 1473 1475 on on IN 1476 1477 a a DT 1478 1490 heterologous heterologous JJ 1491 1499 promoter promoter NN 1500 1503 and and CC 1504 1507 was be VBD 1508 1522 transactivated transactivate VBN 1523 1525 by by IN 1526 1531 c-Rel c-rel NN 1532 1534 or or CC 1535 1538 p65 p65 NN 1538 1539 . . . 1540 1545 Taken take VBN 1546 1554 together together RB 1554 1555 , , , 1556 1559 our our PRP$ 1560 1567 results result NNS 1568 1580 demonstrated demonstrate VBD 1581 1585 that that IN 1586 1593 binding binding NN 1594 1596 of of IN 1597 1606 c-Rel/p65 c-rel/p65 NN 1607 1619 heterodimers heterodimer NNS 1620 1622 to to TO 1623 1624 a a DT 1625 1630 novel novel JJ 1631 1636 kappa kappa NN 1637 1643 B-like b-like JJ 1644 1648 site site NN 1649 1657 mediated mediated JJ 1658 1661 LPS LPS NNP 1662 1671 induction induction NN 1672 1674 of of IN 1675 1677 TF TF NNP 1678 1682 gene gene NN 1683 1693 expression expression NN 1694 1696 in in IN 1697 1706 monocytic monocytic JJ 1707 1712 cells cell NNS 1712 1713 . . .