0 12 Differential differential JJ 13 27 autoregulation autoregulation NN 28 30 of of IN 31 45 glucocorticoid glucocorticoid NN 46 54 receptor receptor NN 55 65 expression expression NN 66 68 in in IN 69 74 human human JJ 75 77 T- t- NN 78 81 and and CC 82 88 B-cell b-cell NN 89 94 lines line NNS 94 95 . . . 97 107 Regulation regulation NN 108 110 of of IN 111 125 glucocorticoid glucocorticoid NN 126 134 receptor receptor NN 135 136 ( ( ( 136 138 GR GR NNP 138 139 ) ) ) 140 150 expression expression NN 151 153 by by IN 154 157 its its PRP$ 158 165 cognate cognate JJ 166 172 ligand ligand NN 173 176 was be VBD 177 185 examined examine VBN 186 188 in in IN 189 192 the the DT 193 217 glucocorticoid-sensitive glucocorticoid-sensitive JJ 218 223 human human JJ 224 232 leukemic leukemic JJ 233 239 T-cell t-cell NN 240 244 line line NN 245 251 6TG1.1 6tg1.1 NN 252 255 and and CC 256 258 in in IN 259 262 the the DT 263 268 human human JJ 269 275 B-cell b-cell NN 276 280 line line NN 281 285 IM-9 im-9 NN 285 286 . . . 287 289 In in IN 290 298 contrast contrast NN 299 301 to to TO 302 305 the the DT 306 314 decrease decrease NN 315 317 in in IN 318 320 GR GR NNP 321 325 mRNA mRNA NNP 326 330 seen see VBN 331 333 in in IN 334 338 IM-9 IM-9 NNP 339 344 cells cell NNS 345 350 after after IN 351 360 treatment treatment NN 361 365 with with IN 366 367 1 1 CD 368 374 microM microm NN 375 388 dexamethasone dexamethasone NN 389 392 for for IN 393 398 16-18 16-18 CD 399 400 h h NN 400 401 , , , 402 411 treatment treatment NN 412 414 of of IN 415 421 6TG1.1 6tg1.1 NN 422 427 cells cell NNS 428 436 resulted result VBD 437 439 in in IN 440 442 an an DT 443 449 8-fold 8-fold JJ 450 458 increase increase NN 459 461 in in IN 462 464 GR GR NNP 465 469 mRNA mRNA NNP 469 470 , , , 471 473 as as IN 474 484 determined determine VBN 485 487 by by IN 488 496 Northern northern NN 497 501 blot blot NN 502 505 and and CC 506 511 RNase rnase NN 512 522 protection protection NN 523 531 analysis analysis NN 531 532 , , , 533 537 with with IN 538 539 a a DT 540 553 corresponding corresponding JJ 554 556 3- 3- CD 557 559 to to TO 560 566 4-fold 4-fold JJ 567 575 increase increase NN 576 578 in in IN 579 581 GR GR NNP 582 589 protein protein NN 589 590 . . . 591 603 Half-maximal half-maximal JJ 604 613 induction induction NN 614 616 of of IN 617 619 GR GR NNP 620 624 mRNA mRNA NNP 625 628 and and CC 629 636 protein protein NN 637 639 in in IN 640 646 6TG1.1 6tg1.1 NN 647 652 cells cell NNS 653 656 was be VBD 657 665 observed observe VBN 666 673 between between IN 674 680 10-100 10-100 CD 681 683 nM nm NN 684 697 dexamethasone dexamethasone NN 697 698 , , , 699 702 and and CC 703 712 inclusion inclusion NN 713 715 of of IN 716 717 1 1 CD 718 724 microM microm NN 725 727 RU RU NNP 728 733 38486 38486 CD 734 744 completely completely RB 745 752 blocked block VBD 753 756 the the DT 757 764 effects effect NNS 765 767 of of IN 768 771 100 100 CD 772 774 nM nm NN 775 788 dexamethasone dexamethasone NN 788 789 , , , 790 803 demonstrating demonstrate VBG 804 808 that that IN 809 817 positive positive JJ 818 832 autoregulation autoregulation NN 833 835 of of IN 836 838 GR GR NNP 839 849 expression expression NN 850 852 in in IN 853 859 6TG1.1 6tg1.1 NN 860 865 cells cell NNS 866 868 is be VBZ 869 870 a a DT 871 888 receptor-mediated receptor-mediated JJ 889 897 response response NN 897 898 . . . 899 907 Positive positive JJ 908 922 autoregulation autoregulation NN 923 925 of of IN 926 928 GR GR NNP 929 939 expression expression NN 940 943 was be VBD 944 948 also also RB 949 957 observed observe VBN 958 960 in in IN 961 985 glucocorticoid-resistant glucocorticoid-resistant JJ 986 992 CEM-C1 cem-c1 NN 993 998 cells cell NNS 998 999 , , , 1000 1005 which which WDT 1006 1013 contain contain VBP 1014 1024 functional functional JJ 1025 1027 GR gr NN 1027 1028 , , , 1029 1032 but but CC 1033 1038 whose whose WP$ 1039 1045 growth growth NN 1046 1048 is be VBZ 1049 1059 unaffected unaffected JJ 1060 1062 by by IN 1063 1078 glucocorticoids glucocorticoid NNS 1078 1079 . . . 1080 1084 Thus thus RB 1084 1085 , , , 1086 1094 positive positive JJ 1095 1109 autoregulation autoregulation NN 1110 1112 is be VBZ 1113 1120 neither neither CC 1121 1122 a a DT 1123 1134 consequence consequence NN 1135 1138 nor nor CC 1139 1142 the the DT 1143 1147 sole sole JJ 1148 1153 cause cause NN 1154 1156 of of IN 1157 1163 growth growth NN 1164 1170 arrest arrest NN 1170 1171 . . . 1172 1175 The the DT 1176 1182 degree degree NN 1183 1185 of of IN 1186 1194 negative negative JJ 1195 1209 autoregulation autoregulation NN 1210 1212 in in IN 1213 1217 IM-9 IM-9 NNP 1218 1223 cells cell NNS 1224 1227 and and CC 1228 1236 positive positive JJ 1237 1251 autoregulation autoregulation NN 1252 1254 in in IN 1255 1261 6TG1.1 6tg1.1 NN 1262 1267 cells cell NNS 1268 1271 was be VBD 1272 1282 unaffected unaffected JJ 1283 1285 by by IN 1286 1296 inhibition inhibition NN 1297 1299 of of IN 1300 1307 protein protein NN 1308 1317 synthesis synthesis NN 1318 1322 with with IN 1323 1336 cycloheximide cycloheximide NN 1336 1337 . . . 1338 1349 Measurement Measurement NNP 1350 1352 of of IN 1353 1355 GR GR NNP 1356 1360 mRNA mrna NN 1361 1369 turnover turnover NN 1370 1372 in in IN 1373 1379 6TG1.1 6tg1.1 NN 1380 1385 cells cell NNS 1386 1393 treated treat VBN 1394 1398 with with IN 1399 1412 actinomycin-D actinomycin-d NN 1413 1421 revealed reveal VBD 1422 1423 a a DT 1424 1433 half-life half-life NN 1434 1436 of of IN 1437 1440 2.5 2.5 CD 1441 1442 h h NN 1442 1443 , , , 1444 1449 which which WDT 1450 1453 was be VBD 1454 1464 unaffected unaffected JJ 1465 1467 by by IN 1468 1481 dexamethasone dexamethasone NN 1482 1491 treatment treatment NN 1491 1492 . . . 1493 1494 A a DT 1495 1502 similar similar JJ 1503 1512 half-life half-life NN 1513 1516 was be VBD 1517 1527 determined determine VBN 1528 1530 in in IN 1531 1535 IM-9 IM-9 NNP 1536 1541 cells cell NNS 1542 1545 and and CC 1546 1549 was be VBD 1550 1554 also also RB 1555 1565 unaffected unaffected JJ 1566 1568 by by IN 1569 1576 steroid steroid NN 1577 1586 treatment treatment NN 1586 1587 . . . 1588 1593 These these DT 1594 1601 results result NNS 1602 1605 are be VBP 1606 1616 consistent consistent JJ 1617 1621 with with IN 1622 1625 the the DT 1626 1640 interpretation interpretation NN 1641 1645 that that IN 1646 1669 glucocorticoid-mediated glucocorticoid-mediated JJ 1670 1684 autoregulation autoregulation NN 1685 1687 of of IN 1688 1690 GR GR NNP 1691 1701 expression expression NN 1702 1704 is be VBZ 1705 1706 a a DT 1707 1722 tissue-specific tissue-specific JJ 1723 1730 primary primary JJ 1731 1746 transcriptional transcriptional JJ 1747 1755 response response NN 1755 1756 . . .