0 5 Human human JJ 6 15 CD3-CD16+ cd3-cd16+ JJ 16 23 natural natural JJ 24 30 killer killer NN 31 36 cells cell NNS 37 44 express express VBP 45 48 the the DT 49 56 hGATA-3 hgata-3 NN 57 58 T t NN 59 63 cell cell NN 64 77 transcription transcription NN 78 84 factor factor NN 85 88 and and CC 89 91 an an DT 92 104 unrearranged unrearranged JJ 105 111 2.3-kb 2.3-kb JJ 112 115 TcR tcr NN 116 121 delta delta NN 122 132 transcript transcript NN 132 133 . . . 135 137 In in IN 138 142 this this DT 143 148 study study NN 149 151 we we PRP 152 160 analyzed analyze VBD 161 164 the the DT 165 166 T t NN 167 171 cell cell NN 172 185 receptor(TcR) receptor(tcr) NN 186 191 delta delta NN 192 203 transcripts transcript NNS 204 213 expressed express VBN 214 216 by by IN 217 226 CD3-CD16+ cd3-cd16+ JJ 227 232 cells cell NNS 233 236 and and CC 237 239 we we PRP 240 252 investigated investigate VBD 253 260 whether whether IN 261 266 these these DT 267 272 cells cell NNS 273 282 expressed express VBD 283 286 the the DT 287 294 hGATA-3 hgata-3 NN 295 296 T t NN 297 301 cell cell NN 302 315 transcription transcription NN 316 322 factor factor NN 323 326 and and CC 327 330 the the DT 331 355 recombination-activating recombination-activating JJ 356 360 gene gene NN 361 362 ( ( ( 362 368 RAG)-1 rag)-1 NN 368 369 . . . 370 378 Multiple multiple JJ 379 382 TcR tcr NN 383 388 delta delta NN 389 400 transcripts transcript NNS 401 409 deriving derive VBG 410 414 from from IN 415 417 an an DT 418 430 unrearranged unrearranged JJ 431 434 TcR tcr NN 435 440 delta delta NN 441 445 gene gene NN 446 450 were be VBD 451 459 detected detect VBN 460 462 in in IN 463 467 both both CC 468 478 polyclonal polyclonal JJ 479 482 and and CC 483 489 clonal clonal JJ 490 499 CD3-CD16+ cd3-cd16+ JJ 500 507 natural natural JJ 508 518 killer(NK) killer(nk) NN 519 523 cell cell NN 524 529 lines line NNS 529 530 . . . 531 534 Two two CD 535 547 unrearranged unrearranged JJ 548 551 TcR tcr NN 552 557 delta delta NN 558 569 transcripts transcript NNS 570 573 had have VBD 574 575 a a DT 576 580 size size NN 581 588 similar similar JJ 589 591 to to TO 592 596 that that DT 597 599 of of IN 600 603 the the DT 604 614 functional functional JJ 615 618 TcR tcr NN 619 624 delta delta NN 625 629 mRNA mRNA NNP 630 631 ( ( ( 631 634 2.3 2.3 CD 635 638 and and CC 639 642 1.3 1.3 CD 643 645 kb kb NN 645 646 ) ) ) 647 652 found find VBN 653 655 in in IN 656 659 TcR TcR NNP 660 672 gamma/delta+ gamma/delta+ NNP 673 674 T t NN 675 686 lymphocytes lymphocyte NNS 686 687 . . . 688 696 Sequence sequence NN 697 705 analysis analysis NN 706 708 of of IN 709 713 nine nine CD 714 723 different different JJ 724 730 2.3-kb 2.3-kb JJ 731 735 cDNA cdna NN 736 742 clones clone NNS 743 751 obtained obtain VBN 752 756 from from IN 757 767 NK-derived nk-derived JJ 768 774 polyA+ polya+ JJ 775 778 RNA RNA NNP 779 788 confirmed confirm VBD 789 793 that that IN 794 798 they they PRP 799 811 corresponded correspond VBD 812 814 to to TO 815 817 an an DT 818 830 unrearranged unrearranged JJ 831 834 TcR tcr NN 835 840 delta delta NN 841 845 gene gene NN 845 846 . . . 847 852 These these DT 853 857 cDNA cDNA NNP 858 862 were be VBD 863 867 2343 2343 CD 868 870 bp bp NN 871 875 long long JJ 876 879 and and CC 880 885 their their PRP$ 886 899 transcription transcription NN 900 910 initiation initiation NN 911 915 site site NN 916 919 was be VBD 920 927 located located JJ 928 931 814 814 CD 932 934 bp bp NN 935 943 upstream upstream RB 944 948 from from IN 949 952 the the DT 953 954 J J NNP 955 960 delta delta NN 961 962 1 1 CD 963 970 segment segment NN 970 971 . . . 972 975 The the DT 976 984 sequence sequence NN 985 992 located located JJ 993 1001 upstream upstream RB 1002 1004 of of IN 1005 1008 the the DT 1009 1010 J J NNP 1011 1016 delta delta NN 1017 1018 1 1 CD 1019 1026 segment segment NN 1027 1039 corresponded correspond VBD 1040 1042 to to TO 1043 1046 the the DT 1047 1057 previously previously RB 1058 1066 reported report VBN 1067 1076 germ-line germ-line NN 1077 1085 sequence sequence NN 1085 1086 . . . 1087 1090 The the DT 1091 1092 J J NNP 1093 1098 delta delta NN 1099 1100 1 1 CD 1101 1108 segment segment NN 1109 1112 was be VBD 1113 1122 correctly correctly RB 1123 1130 spliced splice VBN 1131 1133 to to TO 1134 1135 C C NNP 1136 1141 delta delta NN 1141 1142 ; ; : 1143 1145 in in IN 1146 1154 addition addition NN 1155 1158 the the DT 1159 1163 four four CD 1164 1165 C C NNP 1166 1171 delta delta NN 1172 1177 exons exon NNS 1178 1182 were be VBD 1183 1188 found find VBN 1189 1191 to to TO 1192 1194 be be VB 1195 1202 already already RB 1203 1212 assembled assemble VBN 1212 1213 . . . 1214 1217 Two two CD 1218 1233 polyadenylation polyadenylation NN 1234 1239 sites site NNS 1240 1244 were be VBD 1245 1252 present present JJ 1253 1255 in in IN 1256 1259 the the DT 1260 1266 fourth fourth JJ 1267 1268 C C NNP 1269 1274 delta delta NN 1275 1279 exon exon NN 1279 1280 . . . 1281 1288 However however RB 1288 1289 , , , 1290 1294 only only RB 1295 1299 that that RB 1300 1307 located located JJ 1308 1310 at at IN 1311 1314 the the DT 1315 1316 3 3 CD 1316 1317 ’ ' SYM 1318 1321 end end NN 1322 1330 appeared appear VBD 1331 1333 to to TO 1334 1336 be be VB 1337 1345 utilized utilize VBN 1346 1348 in in IN 1349 1352 the the DT 1353 1359 2.3-kb 2.3-kb JJ 1360 1364 cDNA cdna NN 1364 1365 . . . 1366 1369 The the DT 1370 1380 expression expression NN 1381 1383 of of IN 1384 1391 hGATA-3 hgata-3 NN 1391 1392 , , , 1393 1394 a a DT 1395 1396 T t NN 1397 1410 cell-specific cell-specific JJ 1411 1417 factor factor NN 1418 1423 known know VBN 1424 1426 to to TO 1427 1429 be be VB 1430 1438 involved involve VBN 1439 1441 in in IN 1442 1445 the the DT 1446 1456 regulation regulation NN 1457 1459 of of IN 1460 1463 the the DT 1464 1477 transcription transcription NN 1478 1480 of of IN 1481 1484 TcR TcR NNP 1485 1490 delta delta NN 1491 1496 locus locus NN 1496 1497 , , , 1498 1501 was be VBD 1502 1510 analyzed analyze VBN 1511 1513 by by IN 1514 1522 Northern northern NN 1523 1527 blot blot NN 1527 1528 , , , 1529 1531 in in IN 1532 1540 cultured culture VBN 1541 1543 NK NK NNP 1544 1548 cell cell NN 1549 1559 population population NN 1560 1563 and and CC 1564 1570 clones clone NNS 1571 1572 ( ( ( 1572 1575 but but CC 1576 1579 not not RB 1580 1582 in in IN 1583 1590 freshly freshly RB 1591 1598 derived derive VBN 1599 1603 cell cell NN 1604 1615 populations population NNS 1615 1616 ) ) ) 1616 1617 . . . 1618 1621 All all DT 1622 1624 NK nk NN 1625 1631 clones clone NNS 1632 1635 and and CC 1636 1640 cell cell NN 1641 1646 lines line NNS 1647 1654 studied study VBN 1655 1659 were be VBD 1660 1665 found find VBN 1666 1668 to to TO 1669 1676 express express VB 1677 1693 hGATA-3-specific hgata-3-specific JJ 1694 1698 mRNA mrna NN 1698 1699 , , , 1700 1710 suggesting suggest VBG 1711 1715 that that IN 1716 1723 hGATA-3 hgata-3 NN 1724 1727 may may MD 1728 1730 be be VB 1731 1739 involved involve VBN 1740 1742 in in IN 1743 1746 the the DT 1747 1757 regulation regulation NN 1758 1760 of of IN 1761 1764 the the DT 1765 1777 unrearranged unrearranged JJ 1778 1781 TcR tcr NN 1782 1787 delta delta NN 1788 1792 gene gene NN 1793 1803 expression expression NN 1804 1806 in in IN 1807 1809 NK NK NNP 1810 1815 cells cell NNS 1815 1816 . . . 1817 1824 Finally finally RB 1824 1825 , , , 1826 1828 no no DT 1829 1842 transcription transcription NN 1843 1845 of of IN 1846 1849 the the DT 1850 1855 RAG-1 rag-1 NN 1856 1860 gene gene NN 1861 1866 could could MD 1867 1869 be be VB 1870 1878 detected detect VBN 1879 1881 in in IN 1882 1885 all all DT 1886 1888 NK NK NNP 1889 1893 cell cell NN 1894 1899 lines line NNS 1900 1902 or or CC 1903 1909 clones clone NNS 1910 1918 analyzed analyze VBN 1918 1919 . . .