0 13 Interleukin-3 interleukin-3 NN 14 24 expression expression NN 25 27 by by IN 28 37 activated activate VBN 38 39 T t NN 40 45 cells cell NNS 46 54 involves involve VBZ 55 57 an an DT 58 67 inducible inducible JJ 67 68 , , , 69 84 T-cell-specific t-cell-specific JJ 85 91 factor factor NN 92 95 and and CC 96 98 an an DT 99 106 octamer octamer NN 107 114 binding binding NN 115 122 protein protein NN 122 123 . . . 125 138 Interleukin-3 interleukin-3 NN 139 140 ( ( ( 140 144 IL-3 IL-3 NNP 144 145 ) ) ) 146 148 is be VBZ 149 160 exclusively exclusively RB 161 170 expressed express VBN 171 173 by by IN 174 183 activated activate VBN 184 185 T t NN 186 189 and and CC 190 197 natural natural JJ 198 204 killer killer NN 205 210 cells cell NNS 210 211 , , , 212 213 a a DT 214 222 function function NN 223 227 that that WDT 228 230 is be VBZ 231 238 tightly tightly RB 239 249 controlled control VBN 250 254 both both CC 255 257 in in IN 258 259 a a DT 260 276 lineage-specific lineage-specific JJ 277 280 and and CC 281 283 in in IN 284 285 a a DT 286 307 stimulation-dependent stimulation-dependent JJ 308 314 manner manner NN 314 315 . . . 316 318 We we PRP 319 323 have have VBP 324 336 investigated investigate VBN 337 340 the the DT 341 348 protein protein NN 349 356 binding binding NN 357 372 characteristics characteristic NNS 373 376 and and CC 377 387 functional functional JJ 388 398 importance importance NN 399 401 of of IN 402 405 the the DT 406 422 ACT-1-activating act-1-activating JJ 423 429 region region NN 430 432 of of IN 433 436 the the DT 437 441 IL-3 il-3 NN 442 450 promoter promoter NN 450 451 . . . 452 456 This this DT 457 463 region region NN 464 469 binds bind VBZ 470 472 an an DT 473 482 inducible inducible JJ 482 483 , , , 484 499 T-cell-specific t-cell-specific JJ 500 506 factor factor NN 507 511 over over IN 512 515 its its PRP$ 516 517 5 5 CD 517 518 ’ ' SYM 519 522 end end NN 522 523 , , , 524 525 a a DT 526 530 site site NN 531 535 that that WDT 536 538 is be VBZ 539 548 necessary necessary JJ 549 552 for for IN 553 556 the the DT 557 567 expression expression NN 568 570 of of IN 571 575 IL-3 il-3 NN 576 578 in in IN 579 582 the the DT 583 590 absence absence NN 591 593 of of IN 594 599 other other JJ 600 608 upstream upstream JJ 609 617 elements element NNS 617 618 . . . 619 623 Over over IN 624 627 its its PRP$ 628 629 3 3 CD 629 630 ’ ' SYM 631 634 end end NN 634 635 , , , 636 638 it it PRP 639 644 binds bind VBZ 645 646 a a DT 647 653 factor factor NN 654 658 that that WDT 659 661 is be VBZ 662 674 ubiquitously ubiquitously RB 675 678 and and CC 679 693 constitutively constitutively RB 694 703 expressed express VBN 703 704 . . . 705 709 This this DT 710 716 factor factor NN 717 719 is be VBZ 720 725 Oct-1 oct-1 NN 726 728 or or CC 729 731 an an DT 732 747 immunologically immunologically RB 748 755 related related JJ 756 771 octamer-binding octamer-binding JJ 772 779 protein protein NN 779 780 , , , 781 784 and and CC 785 787 it it PRP 788 793 plays play VBZ 794 795 a a DT 796 800 role role NN 801 803 in in IN 804 816 coordinating coordinate VBG 817 820 the the DT 821 829 activity activity NN 830 832 of of IN 833 840 several several JJ 841 851 regulatory regulatory JJ 852 860 elements element NNS 860 861 . . . 862 867 These these DT 868 883 characteristics characteristic NNS 884 888 make make VBP 889 892 the the DT 893 898 ACT-1 act-1 NN 899 903 site site NN 904 913 analogous analogous JJ 914 916 to to TO 917 920 the the DT 921 931 activating activate VBG 932 938 ARRE-1 arre-1 NN 939 943 site site NN 944 946 in in IN 947 950 the the DT 951 955 IL-2 il-2 NN 956 964 promoter promoter NN 964 965 . . . 966 977 Furthermore furthermore RB 977 978 , , , 979 982 and and CC 983 990 despite despite IN 991 992 a a DT 993 997 lack lack NN 998 1000 of of IN 1001 1009 sequence sequence NN 1010 1018 homology homology NN 1018 1019 , , , 1020 1023 the the DT 1024 1033 promoters promoter NNS 1034 1036 of of IN 1037 1041 IL-3 IL-3 NNP 1042 1045 and and CC 1046 1050 IL-2 il-2 NN 1051 1056 share share VBP 1057 1059 an an DT 1060 1074 organizational organizational JJ 1075 1082 pattern pattern NN 1083 1085 of of IN 1086 1096 regulatory regulatory JJ 1097 1105 elements element NNS 1106 1110 that that WDT 1111 1113 is be VBZ 1114 1120 likely likely JJ 1121 1123 to to TO 1124 1126 be be VB 1127 1136 important important JJ 1137 1140 for for IN 1141 1144 the the DT 1145 1160 T-cell-specific t-cell-specific JJ 1161 1171 expression expression NN 1172 1174 of of IN 1175 1180 these these DT 1181 1186 genes gene NNS 1186 1187 . . .