0	3	ras	ras	NN
4	11	protein	protein	NN
12	20	activity	activity	NN
21	23	is	be	VBZ
24	33	essential	essential	JJ
34	37	for	for	IN
38	44	T-cell	t-cell	NN
45	52	antigen	antigen	NN
53	61	receptor	receptor	NN
62	68	signal	signal	NN
69	81	transduction	transduction	NN
81	82	.	.	.

84	86	In	in	IN
87	88	a	a	DT
89	95	Jurkat	Jurkat	NNP
96	100	cell	cell	NN
101	106	model	model	NN
107	109	of	of	IN
110	116	T-cell	t-cell	NN
117	127	activation	activation	NN
128	130	an	an	DT
131	144	interleukin-2	interleukin-2	NN
145	162	promoter/reporter	promoter/reporter	NN
163	167	gene	gene	NN
168	177	construct	construct	NN
178	181	was	be	VBD
182	191	activated	activate	VBN
192	194	by	by	IN
195	202	antigen	antigen	NN
203	211	receptor	receptor	NN
212	219	agonism	agonism	NN
220	222	in	in	IN
223	234	combination	combination	NN
235	239	with	with	IN
240	243	the	the	DT
244	254	lymphokine	lymphokine	NN
255	268	interleukin-1	interleukin-1	NN
268	269	.	.	.

270	277	Antigen	antigen	NN
278	286	receptor	receptor	NN
287	294	signals	signal	NNS
295	300	could	could	MD
301	303	be	be	VB
304	312	mimicked	mimic	VBN
313	315	by	by	IN
316	326	suboptimal	suboptimal	JJ
327	337	activation	activation	NN
338	340	of	of	IN
341	348	protein	protein	NN
349	355	kinase	kinase	NNP
356	357	C	C	NNP
358	359	(	(	(
359	362	PKC	PKC	NNP
362	363	)	)	)
364	368	with	with	IN
369	376	phorbol	phorbol	NN
377	383	esters	ester	NNS
384	386	in	in	IN
387	398	combination	combination	NN
399	403	with	with	IN
404	411	calcium	calcium	NN
412	424	mobilization	mobilization	NN
425	427	by	by	IN
428	430	an	an	DT
431	440	ionophore	ionophore	NN
440	441	.	.	.

442	444	In	in	IN
445	459	cotransfection	cotransfection	NN
460	471	experiments	experiment	NNS
471	472	,	,	,
473	482	oncogenic	oncogenic	JJ
483	487	rats	rat	NNS
488	496	obviated	obviate	VBD
497	500	the	the	DT
501	505	need	need	NN
506	509	for	for	IN
510	513	PKC	PKC	NNP
514	525	stimulation	stimulation	NN
526	529	but	but	CC
530	533	did	do	VBD
534	537	not	not	RB
538	545	replace	replace	VB
546	552	either	either	CC
553	556	the	the	DT
557	564	calcium	calcium	NN
565	571	signal	signal	NN
572	574	or	or	CC
575	588	interleukin-1	interleukin-1	NN
588	589	.	.	.

590	599	Activated	activate	VBN
600	603	ras	ras	NN
604	614	expression	expression	NN
615	619	also	also	RB
620	628	replaced	replace	VBD
629	632	the	the	DT
633	644	requirement	requirement	NN
645	648	for	for	IN
649	652	PKC	PKC	NNP
653	664	stimulation	stimulation	NN
665	667	in	in	IN
668	678	activation	activation	NN
679	681	of	of	IN
682	685	the	the	DT
686	692	T-cell	t-cell	NN
693	706	transcription	transcription	NN
707	713	factor	factor	NN
714	719	NF-AT	nf-at	NN
719	720	.	.	.

721	722	A	a	DT
723	731	dominant	dominant	JJ
732	742	inhibitory	inhibitory	JJ
743	746	ras	ras	NN
747	753	mutant	mutant	NN
754	766	specifically	specifically	RB
767	774	blocked	block	VBD
775	782	antigen	antigen	NN
783	791	receptor	receptor	NN
792	799	agonism	agonism	NN
799	800	,	,	,
801	811	indicating	indicate	VBG
812	816	that	that	IN
817	820	ras	ras	NN
821	829	activity	activity	NN
830	832	is	be	VBZ
833	841	required	require	VBN
842	845	for	for	IN
846	853	antigen	antigen	NN
854	862	receptor	receptor	NN
863	872	signaling	signaling	NN
872	873	.	.	.

874	876	In	in	IN
877	885	addition	addition	NN
885	886	,	,	,
887	889	an	an	DT
890	899	inhibitor	inhibitor	NN
900	902	of	of	IN
903	906	PKC	PKC	NNP
907	914	blocked	block	VBD
915	919	both	both	CC
920	929	activated	activate	VBD
930	933	ras	ras	NN
934	937	and	and	CC
938	945	phorbol	phorbol	NN
946	951	ester	ester	NN
952	963	stimulation	stimulation	NN
963	964	,	,	,
965	975	suggesting	suggest	VBG
976	977	a	a	DT
978	982	role	role	NN
983	986	for	for	IN
987	990	ras	ras	NN
991	999	upstream	upstream	JJ
1000	1002	of	of	IN
1003	1006	PKC	PKC	NNP
1006	1007	.	.	.