0	8	Protease	Protease	NNP
9	18	treatment	treatment	NN
19	21	of	of	IN
22	29	nuclear	nuclear	JJ
30	38	extracts	extract	NNS
39	52	distinguishes	distinguish	VBZ
53	60	between	between	IN
61	66	class	class	NN
67	69	II	ii	CD
70	73	MHC	MHC	NNP
74	76	X1	X1	NNP
77	80	box	box	NN
81	92	DNA-binding	dna-binding	JJ
93	101	proteins	protein	NNS
102	104	in	in	IN
105	114	wild-type	wild-type	JJ
115	118	and	and	CC
119	124	class	class	NN
125	137	II-deficient	ii-deficient	JJ
138	139	B	B	NNP
140	145	cells	cell	NNS
145	146	.	.	.

148	151	The	the	DT
152	153	X	X	NNP
154	157	box	box	NN
158	164	region	region	NN
165	167	is	be	VBZ
168	176	critical	critical	JJ
177	180	for	for	IN
181	190	directing	direct	VBG
191	194	the	the	DT
195	205	expression	expression	NN
206	208	of	of	IN
209	214	class	class	NN
215	217	II	ii	CD
218	223	major	major	JJ
224	242	histocompatibility	histocompatibility	NN
243	250	complex	complex	NN
251	256	genes	gene	NNS
257	259	in	in	IN
260	261	B	B	NNP
262	273	lymphocytes	lymphocyte	NNS
273	274	.	.	.

275	283	Although	although	IN
284	291	several	several	JJ
292	297	class	class	NN
298	300	II	ii	CD
301	318	promoter-specific	promoter-specific	JJ
319	322	DNA	dna	NN
323	330	binding	binding	NN
331	338	factors	factor	NNS
339	343	have	have	VBP
344	348	been	be	VBN
349	358	described	describe	VBN
358	359	,	,	,
360	364	only	only	RB
365	368	the	the	DT
369	370	X	X	NNP
371	374	box	box	NN
375	381	region	region	NN
382	388	factor	factor	NN
388	389	,	,	,
390	393	RFX	RFX	NNP
393	394	,	,	,
395	400	shows	show	VBZ
401	402	a	a	DT
403	410	genetic	genetic	JJ
411	422	correlation	correlation	NN
423	427	with	with	IN
428	433	class	class	NN
434	436	II	ii	CD
437	447	expression	expression	NN
447	448	,	,	,
449	454	being	be	VBG
455	464	deficient	deficient	JJ
465	467	in	in	IN
468	472	some	some	DT
473	474	B	B	NNP
475	479	cell	cell	NN
480	485	lines	line	NNS
486	493	derived	derive	VBN
494	498	from	from	IN
499	507	patients	patient	NNS
508	512	with	with	IN
513	518	class	class	NN
519	531	II-deficient	ii-deficient	JJ
532	542	congenital	congenital	JJ
543	559	immunodeficiency	immunodeficiency	NN
559	560	.	.	.

561	563	To	to	TO
564	571	further	further	RB
572	580	evaluate	evaluate	VB
581	584	the	the	DT
585	589	role	role	NN
590	592	of	of	IN
593	594	X	X	NNP
595	598	box	box	NN
599	610	DNA-binding	dna-binding	JJ
611	619	proteins	protein	NNS
620	622	in	in	IN
623	628	class	class	NN
629	631	II	ii	CD
632	636	gene	gene	NN
637	647	expression	expression	NN
647	648	,	,	,
649	652	the	the	DT
653	657	role	role	NN
658	660	of	of	IN
661	664	the	the	DT
665	666	X	X	NNP
667	670	box	box	NN
671	677	region	region	NN
678	681	was	be	VBD
682	690	examined	examine	VBN
691	693	in	in	IN
694	698	both	both	CC
699	704	class	class	NN
705	716	II-positive	ii-positive	JJ
717	720	and	and	CC
721	730	-negative	-negative	JJ
731	739	lymphoid	lymphoid	JJ
740	745	cells	cell	NNS
745	746	.	.	.

747	749	In	in	IN
750	758	addition	addition	NN
759	761	to	to	TO
762	765	the	the	DT
766	775	wild-type	wild-type	JJ
776	777	B	B	NNP
778	782	cell	cell	NN
783	787	line	line	NN
788	792	Raji	Raji	NNP
792	793	,	,	,
794	797	two	two	CD
798	803	class	class	NN
804	806	II	ii	CD
807	822	transcriptional	transcriptional	JJ
823	829	mutant	mutant	JJ
830	834	cell	cell	NN
835	840	lines	line	NNS
840	841	,	,	,
842	845	SJO	SJO	NNP
846	849	and	and	CC
850	857	RJ2.2.5	RJ2.2.5	NNP
857	858	,	,	,
859	862	and	and	CC
863	869	Jurkat	Jurkat	NNP
869	870	,	,	,
871	872	a	a	DT
873	878	class	class	NN
879	881	II	ii	CD
882	890	negative	negative	JJ
891	892	T	t	NN
893	897	cell	cell	NN
898	902	line	line	NN
902	903	,	,	,
904	908	were	be	VBD
909	917	examined	examine	VBN
917	918	.	.	.

919	921	In	in	IN
922	930	contrast	contrast	NN
931	933	to	to	TO
934	943	wild-type	wild-type	JJ
944	945	B	B	NNP
946	951	cells	cell	NNS
951	952	,	,	,
953	960	neither	neither	DT
961	963	of	of	IN
964	967	the	the	DT
968	973	class	class	NN
974	976	II	ii	CD
977	983	mutant	mutant	JJ
984	988	cell	cell	NN
989	994	lines	line	NNS
995	1000	could	could	MD
1001	1004	use	use	VB
1005	1008	the	the	DT
1009	1010	X	X	NNP
1011	1014	box	box	NN
1015	1021	region	region	NN
1022	1024	to	to	TO
1025	1031	direct	direct	VB
1032	1035	the	the	DT
1036	1046	expression	expression	NN
1047	1049	of	of	IN
1050	1051	a	a	DT
1052	1063	transiently	transiently	RB
1064	1075	transfected	transfecte	VBN
1076	1084	reporter	reporter	NN
1085	1089	gene	gene	NN
1089	1090	,	,	,
1091	1101	indicating	indicate	VBG
1102	1106	that	that	IN
1107	1110	the	the	DT
1111	1112	X	X	NNP
1113	1126	box-dependent	box-dependent	JJ
1127	1142	transcriptional	transcriptional	JJ
1143	1150	pathway	pathway	NN
1151	1153	is	be	VBZ
1154	1163	defective	defective	JJ
1164	1166	in	in	IN
1167	1172	these	these	DT
1173	1178	cells	cell	NNS
1178	1179	.	.	.

1180	1183	The	the	DT
1184	1191	binding	binding	NN
1192	1200	activity	activity	NN
1201	1203	of	of	IN
1204	1207	the	the	DT
1208	1210	X1	x1	NN
1211	1214	box	box	NN
1215	1226	DNA-binding	dna-binding	JJ
1227	1234	protein	protein	NN
1235	1238	RFX	RFX	NNP
1239	1242	was	be	VBD
1243	1251	examined	examine	VBN
1252	1255	and	and	CC
1256	1261	found	find	VBN
1262	1264	to	to	TO
1265	1267	be	be	VB
1268	1275	present	present	JJ
1276	1278	in	in	IN
1279	1288	wild-type	wild-type	JJ
1289	1290	B	B	NNP
1291	1296	cells	cell	NNS
1297	1300	and	and	CC
1301	1304	the	the	DT
1305	1311	mutant	mutant	JJ
1312	1319	RJ2.2.5	rj2.2.5	NN
1320	1323	but	but	CC
1324	1327	was	be	VBD
1328	1334	absent	absent	JJ
1335	1337	in	in	IN
1338	1341	SJO	SJO	NNP
1342	1345	and	and	CC
1346	1352	Jurkat	Jurkat	NNP
1352	1353	.	.	.

1354	1361	However	however	RB
1361	1362	,	,	,
1363	1368	other	other	JJ
1369	1371	X1	x1	NN
1372	1384	box-specific	box-specific	JJ
1385	1395	activities	activity	NNS
1396	1400	were	be	VBD
1401	1409	detected	detect	VBN
1410	1412	in	in	IN
1413	1416	all	all	DT
1417	1422	these	these	DT
1423	1427	cell	cell	NN
1428	1433	lines	line	NNS
1433	1434	.	.	.

1435	1437	To	to	TO
1438	1447	determine	determine	VB
1448	1455	whether	whether	IN
1456	1461	these	these	DT
1462	1471	different	different	JJ
1472	1474	X1	x1	NN
1475	1478	box	box	NN
1479	1489	activities	activity	NNS
1490	1501	represented	represent	VBD
1502	1510	distinct	distinct	JJ
1511	1514	DNA	dna	NN
1515	1522	binding	binding	NN
1523	1531	proteins	protein	NNS
1532	1534	or	or	CC
1535	1545	multimeric	multimeric	JJ
1546	1551	forms	form	NNS
1552	1554	of	of	IN
1555	1558	the	the	DT
1559	1563	same	same	JJ
1564	1573	factor(s)	factor(s)	NN
1573	1574	,	,	,
1575	1583	protease	protease	NN
1584	1593	treatment	treatment	NN
1594	1596	of	of	IN
1597	1600	the	the	DT
1601	1606	crude	crude	JJ
1607	1614	nuclear	nuclear	JJ
1615	1623	extracts	extract	NNS
1624	1632	followed	follow	VBN
1633	1635	by	by	IN
1636	1647	DNA-binding	dna-binding	JJ
1648	1654	assays	assay	NNS
1655	1659	were	be	VBD
1660	1667	carried	carry	VBN
1668	1671	out	out	RP
1672	1675	and	and	CC
1676	1688	demonstrated	demonstrate	VBD
1689	1693	that	that	IN
1694	1695	B	B	NNP
1696	1700	cell	cell	NN
1701	1709	extracts	extract	NNS
1710	1717	contain	contain	VBP
1718	1720	at	at	IN
1721	1726	least	least	JJS
1727	1730	two	two	CD
1731	1742	X1-specific	x1-specific	JJ
1743	1750	factors	factor	NNS
1750	1751	.	.	.

1752	1755	One	one	CD
1756	1758	of	of	IN
1759	1764	these	these	DT
1765	1772	cleaved	cleave	VBN
1773	1781	products	product	NNS
1782	1783	(	(	(
1783	1787	band	band	NN
1788	1789	1	1	CD
1790	1792	pk	pk	NN
1792	1793	)	)	)
1794	1804	correlates	correlate	VBZ
1805	1809	with	with	IN
1810	1813	RFX	RFX	NNP
1814	1822	activity	activity	NN
1822	1823	.	.	.

1824	1825	A	a	DT
1826	1833	similar	similar	JJ
1834	1844	comparison	comparison	NN
1845	1849	with	with	IN
1850	1866	protease-treated	protease-treated	JJ
1867	1875	extracts	extract	NNS
1876	1884	prepared	prepare	VBN
1885	1889	from	from	IN
1890	1896	Jurkat	Jurkat	NNP
1897	1902	cells	cell	NNS
1903	1915	demonstrated	demonstrate	VBD
1916	1919	the	the	DT
1920	1928	presence	presence	NN
1929	1931	of	of	IN
1932	1935	the	the	DT
1936	1940	band	band	NN
1941	1944	1pk	1pk	NN
1945	1953	activity	activity	NN
1954	1961	despite	despite	IN
1962	1964	an	an	DT
1965	1972	absence	absence	NN
1973	1975	of	of	IN
1976	1979	the	the	DT
1980	1986	native	native	JJ
1987	1990	RFX	rfx	NN
1991	1999	activity	activity	NN
1999	2000	.	.	.

2001	2003	In	in	IN
2004	2012	contrast	contrast	NN
2012	2013	,	,	,
2014	2022	protease	protease	NN
2023	2032	treatment	treatment	NN
2033	2036	and	and	CC
2037	2045	analysis	analysis	NN
2046	2048	of	of	IN
2049	2052	SJO	SJO	NNP
2053	2061	extracts	extract	NNS
2062	2068	showed	show	VBD
2069	2071	no	no	DT
2072	2082	detectable	detectable	JJ
2083	2089	levels	level	NNS
2090	2092	of	of	IN
2093	2096	the	the	DT
2097	2101	band	band	NN
2102	2105	1pk	1pk	NN
2106	2114	activity	activity	NN
2114	2115	.	.	.

2116	2121	These	these	DT
2122	2129	results	result	NNS
2130	2141	demonstrate	demonstrate	VBP
2142	2146	that	that	IN
2147	2155	multiple	multiple	JJ
2156	2158	X1	x1	NN
2159	2171	box-specific	box-specific	JJ
2172	2183	DNA-binding	dna-binding	JJ
2184	2194	activities	activity	NNS
2195	2200	exist	exist	VBP
2201	2203	in	in	IN
2204	2207	all	all	DT
2208	2216	lymphoid	lymphoid	JJ
2217	2222	cells	cell	NNS
2222	2223	,	,	,
2224	2227	but	but	CC
2228	2231	the	the	DT
2232	2240	presence	presence	NN
2241	2243	of	of	IN
2244	2246	an	an	DT
2247	2255	actively	actively	RB
2256	2263	binding	bind	VBG
2264	2267	RFX	RFX	NNP
2268	2275	species	specie	NNS
2276	2286	correlates	correlate	VBZ
2287	2291	with	with	IN
2292	2297	class	class	NN
2298	2300	II	ii	CD
2301	2314	transcription	transcription	NN
2314	2315	.	.	.