0 10 Expression expression NN 11 13 of of IN 14 19 PILOT PILOT NNP 19 20 , , , 21 22 a a DT 23 31 putative putative JJ 32 45 transcription transcription NN 46 52 factor factor NN 52 53 , , , 54 62 requires require VBZ 63 66 two two CD 67 74 signals signal NNS 75 78 and and CC 79 81 is be VBZ 82 93 cyclosporin cyclosporin NN 94 95 A a NN 96 105 sensitive sensitive JJ 106 108 in in IN 109 110 T t NN 111 116 cells cell NNS 116 117 . . . 119 122 Few few JJ 123 128 known known JJ 129 134 genes gene NNS 135 136 ( ( ( 136 140 IL-2 IL-2 NNP 140 141 , , , 142 149 members member NNS 150 152 of of IN 153 156 the the DT 157 161 IL-8 il-8 NN 162 168 family family NN 168 169 , , , 170 186 interferon-gamma interferon-gamma NN 186 187 ) ) ) 188 191 are be VBP 192 199 induced induce VBN 200 202 in in IN 203 204 T t NN 205 210 cells cell NNS 211 215 only only RB 216 223 through through IN 224 227 the the DT 228 236 combined combined JJ 237 243 effect effect NN 244 246 of of IN 247 254 phorbol phorbol NN 255 263 myristic myristic JJ 264 271 acetate acetate NN 272 273 ( ( ( 273 276 PMA PMA NNP 276 277 ) ) ) 278 281 and and CC 282 283 a a DT 284 300 Ca(2+)-ionophore Ca(2+)-ionophore NNP 300 301 , , , 302 305 and and CC 306 316 expression expression NN 317 319 of of IN 320 324 only only RB 325 330 these these DT 331 336 genes gene NNS 337 340 can can MD 341 343 be be VB 344 349 fully fully RB 350 360 suppressed suppress VBN 361 363 by by IN 364 375 Cyclosporin Cyclosporin NNP 376 377 A A NNP 378 379 ( ( ( 379 382 CyA CyA NNP 382 383 ) ) ) 383 384 . . . 385 387 We we PRP 388 392 have have VBP 393 403 identified identify VBN 404 405 a a DT 406 414 putative putative JJ 415 428 transcription transcription NN 429 435 factor factor NN 435 436 , , , 437 447 designated designate VBN 448 453 PILOT PILOT NNP 453 454 , , , 455 459 with with IN 460 462 an an DT 463 472 identical identical JJ 473 477 dual dual JJ 478 484 signal signal NN 485 496 requirement requirement NN 497 500 for for IN 501 511 expression expression NN 511 512 . . . 513 522 Induction Induction NNP 523 525 of of IN 526 529 the the DT 530 535 PILOT PILOT NNP 536 540 gene gene NN 541 543 is be VBZ 544 554 detectable detectable JJ 555 557 in in IN 558 563 human human JJ 564 565 T t NN 566 571 cells cell NNS 572 574 20 20 CD 575 578 min min NN 579 588 following follow VBG 589 599 activation activation NN 600 602 in in IN 603 606 the the DT 607 615 presence presence NN 616 618 of of IN 619 632 cycloheximide cycloheximide NN 633 636 and and CC 637 639 is be VBZ 640 645 fully fully RB 646 656 suppressed suppress VBN 657 659 by by IN 660 663 CyA CyA NNP 663 664 . . . 665 668 The the DT 669 674 PILOT pilot NN 675 682 protein protein NN 683 686 has have VBZ 687 688 a a DT 689 699 calculated calculate VBN 700 704 M(r) m(r) NN 705 707 of of IN 708 712 42.6 42.6 CD 713 716 kDa kda NN 717 720 and and CC 721 729 contains contain VBZ 730 735 three three CD 736 740 zinc zinc NN 741 748 fingers finger NNS 749 751 of of IN 752 755 the the DT 756 765 C2H2-type c2h2-type NN 766 768 at at IN 769 772 the the DT 773 790 carboxyl-terminus carboxyl-terminus NN 791 796 which which WDT 797 800 are be VBP 801 807 highly highly RB 808 818 homologous homologous JJ 819 821 to to TO 822 825 the the DT 826 830 zinc zinc NN 831 837 finger finger NN 838 845 regions region NNS 846 848 of of IN 849 852 the the DT 853 866 transcription transcription NN 867 874 factors factor NNS 875 879 EGR1 egr1 NN 879 880 , , , 881 885 EGR2 EGR2 NNP 885 886 , , , 887 890 and and CC 891 894 pAT pat NN 895 898 133 133 CD 898 899 . . . 900 902 In in IN 903 911 contrast contrast NN 912 914 to to TO 915 916 T t NN 917 922 cells cell NNS 922 923 , , , 924 926 in in IN 927 938 fibroblasts fibroblast NNS 939 944 PILOT PILOT NNP 945 949 gene gene NN 950 960 expression expression NN 961 969 requires require VBZ 970 974 only only RB 975 978 one one CD 979 985 signal signal NN 986 987 ( ( ( 987 990 PMA PMA NNP 990 991 ) ) ) 992 995 and and CC 996 998 is be VBZ 999 1002 not not RB 1003 1011 affected affect VBN 1012 1014 by by IN 1015 1018 CyA CyA NNP 1018 1019 . . . 1020 1024 This this DT 1025 1036 observation observation NN 1037 1045 directly directly RB 1046 1058 demonstrates demonstrate VBZ 1059 1062 the the DT 1063 1072 existence existence NN 1073 1075 of of IN 1076 1077 a a DT 1078 1082 Ca2+ ca2+ NN 1083 1099 signal-dependent signal-dependent JJ 1100 1110 regulatory regulatory JJ 1111 1118 element element NN 1119 1129 obligatory obligatory JJ 1130 1133 for for IN 1134 1144 expression expression NN 1145 1147 of of IN 1148 1152 some some DT 1153 1158 genes gene NNS 1159 1161 in in IN 1162 1163 T t NN 1164 1169 cells cell NNS 1170 1173 but but CC 1174 1177 not not RB 1178 1180 in in IN 1181 1192 fibroblasts fibroblast NNS 1192 1193 . . . 1194 1198 This this DT 1199 1211 differential differential JJ 1212 1222 expression expression NN 1223 1228 model model NN 1229 1233 will will MD 1234 1236 be be VB 1237 1245 valuable valuable JJ 1246 1248 in in IN 1249 1252 the the DT 1253 1263 dissection dissection NN 1264 1266 of of IN 1267 1270 the the DT 1271 1275 dual dual JJ 1276 1282 signal signal NN 1283 1290 pathway pathway NN 1291 1293 in in IN 1294 1295 T t NN 1296 1301 cells cell NNS 1302 1305 and and CC 1306 1309 the the DT 1310 1317 effects effect NNS 1318 1320 of of IN 1321 1324 CyA CyA NNP 1325 1329 upon upon IN 1330 1332 it it PRP 1332 1333 . . .