0 11 Suppression suppression NN 12 14 of of IN 15 16 a a DT 17 25 cellular cellular JJ 26 41 differentiation differentiation NN 42 49 program program NN 50 52 by by IN 53 60 phorbol phorbol NN 61 67 esters ester NNS 68 77 coincides coincide VBZ 78 82 with with IN 83 93 inhibition inhibition NN 94 96 of of IN 97 104 binding binding NN 105 107 of of IN 108 109 a a DT 110 123 cell-specific cell-specific JJ 124 137 transcription transcription NN 138 144 factor factor NN 145 146 ( ( ( 146 151 NF-E2 nf-e2 NN 151 152 ) ) ) 153 155 to to TO 156 158 an an DT 159 167 enhancer enhancer NN 168 175 element element NN 176 184 required require VBN 185 188 for for IN 189 199 expression expression NN 200 202 of of IN 203 205 an an DT 206 224 erythroid-specific erythroid-specific JJ 225 229 gene gene NN 229 230 . . . 232 241 Induction Induction NNP 242 244 by by IN 245 250 hemin hemin NN 251 260 increases increase VBZ 260 261 , , , 262 267 while while IN 268 277 induction induction NN 278 282 with with IN 283 319 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 320 321 ( ( ( 321 324 TPA TPA NNP 324 325 ) ) ) 326 335 represses repress VBZ 335 336 , , , 337 355 erythroid-specific erythroid-specific JJ 356 360 gene gene NN 361 371 expression expression NN 372 374 in in IN 375 378 the the DT 379 384 human human JJ 385 389 cell cell NN 390 394 line line NN 395 399 K562 k562 NN 399 400 . . . 401 403 We we PRP 404 412 analyzed analyze VBD 413 416 the the DT 417 424 effects effect NNS 425 427 of of IN 428 433 hemin hemin NN 434 436 or or CC 437 440 TPA TPA NNP 441 450 induction induction NN 451 453 on on IN 454 457 the the DT 458 465 binding binding NN 466 469 and and CC 470 478 activity activity NN 479 481 of of IN 482 495 transcription transcription NN 496 503 factors factor NNS 504 506 at at IN 507 508 a a DT 509 519 regulatory regulatory JJ 520 527 element element NN 528 533 found find VBN 534 540 within within IN 541 544 the the DT 545 560 transcriptional transcriptional JJ 561 571 regulatory regulatory JJ 572 581 sequences sequence NNS 582 584 of of IN 585 589 many many JJ 590 608 erythroid-specific erythroid-specific JJ 609 614 genes gene NNS 614 615 . . . 616 619 TPA TPA NNP 620 629 induction induction NN 630 639 increases increase VBZ 640 643 the the DT 644 651 binding binding NN 652 654 of of IN 655 665 ubiquitous ubiquitous JJ 666 670 AP-1 ap-1 NN 671 678 factors factor NNS 679 681 to to TO 682 686 this this DT 687 694 element element NN 694 695 . . . 696 699 TPA TPA NNP 700 709 induction induction NN 710 718 inhibits inhibit VBZ 719 722 the the DT 723 730 binding binding NN 731 733 of of IN 734 737 the the DT 738 745 lineage lineage NN 746 753 limited limited JJ 754 767 transcription transcription NN 768 774 factor factor NN 775 780 NF-E2 nf-e2 NN 781 783 to to TO 784 788 this this DT 789 804 transcriptional transcriptional JJ 805 812 control control NN 813 820 element element NN 820 821 . . . 822 827 Hemin Hemin NNP 828 837 induction induction NN 838 840 of of IN 841 845 K562 k562 NN 846 851 cells cell NNS 852 856 does do VBZ 857 860 not not RB 861 871 facilitate facilitate VB 872 875 the the DT 876 883 binding binding NN 884 886 of of IN 887 892 NF-E2 nf-e2 NN 893 895 to to TO 896 899 its its PRP$ 900 911 recognition recognition NN 912 916 site site NN 916 917 . . . 918 923 Hemin Hemin NNP 924 933 induction induction NN 934 941 appears appear VBZ 942 944 to to TO 945 960 nonspecifically nonspecifically RB 961 969 increase increase VB 970 973 the the DT 974 984 expression expression NN 985 987 of of IN 988 999 transiently transiently RB 1000 1011 transfected transfecte VBN 1012 1017 genes gene NNS 1018 1020 in in IN 1021 1025 K562 k562 NN 1026 1031 cells cell NNS 1031 1032 . . . 1033 1039 Beyond beyond IN 1040 1044 this this DT 1045 1056 nonspecific nonspecific JJ 1057 1065 increase increase NN 1066 1068 in in IN 1069 1073 gene gene NN 1074 1084 expression expression NN 1084 1085 , , , 1086 1091 hemin hemin NN 1092 1101 induction induction NN 1102 1106 acts act VBZ 1107 1109 to to TO 1110 1118 increase increase VB 1119 1122 the the DT 1123 1131 activity activity NN 1132 1134 of of IN 1135 1138 the the DT 1139 1146 lineage lineage NN 1147 1154 limited limited JJ 1155 1168 transcription transcription NN 1169 1175 factor factor NN 1176 1181 NF-E2 nf-e2 NN 1181 1182 . . . 1183 1186 The the DT 1187 1196 divergent divergent JJ 1197 1204 effects effect NNS 1205 1207 of of IN 1208 1213 hemin hemin NN 1214 1217 and and CC 1218 1221 TPA TPA NNP 1222 1224 on on IN 1225 1229 gene gene NN 1230 1240 expression expression NN 1241 1243 in in IN 1244 1248 K562 k562 NN 1249 1254 cells cell NNS 1255 1258 are be VBP 1259 1267 mediated mediate VBN 1267 1268 , , , 1269 1271 in in IN 1272 1276 part part NN 1276 1277 , , , 1278 1280 by by IN 1281 1286 their their PRP$ 1287 1298 contrasting contrast VBG 1299 1306 effects effect NNS 1307 1309 on on IN 1310 1313 the the DT 1314 1327 transcription transcription NN 1328 1334 factor factor NN 1335 1340 NF-E2 nf-e2 NN 1340 1341 . . .