0	15	Transcriptional	transcriptional	JJ
16	26	regulation	regulation	NN
27	29	of	of	IN
30	33	the	the	DT
34	42	pyruvate	pyruvate	NN
43	49	kinase	kinase	NNP
50	68	erythroid-specific	erythroid-specific	JJ
69	77	promoter	promoter	NN
77	78	.	.	.

80	86	Mammal	mammal	JJ
87	95	pyruvate	pyruvate	NN
96	103	kinases	kinase	NNS
104	107	are	be	VBP
108	115	encoded	encode	VBN
116	118	by	by	IN
119	122	two	two	CD
123	128	genes	gene	NNS
128	129	.	.	.

130	133	The	the	DT
134	135	L	L	NNP
136	140	gene	gene	NN
141	149	produces	produce	VBZ
150	153	the	the	DT
154	163	erythroid	erythroid	JJ
164	165	(	(	(
165	169	R-PK	R-PK	NNP
169	170	)	)	)
171	173	or	or	CC
174	177	the	the	DT
178	185	hepatic	hepatic	JJ
186	187	(	(	(
187	191	L-PK	L-PK	NNP
191	192	)	)	)
193	201	isozymes	isozyme	NNS
202	204	by	by	IN
205	208	the	the	DT
209	220	alternative	alternative	JJ
221	224	use	use	NN
225	227	of	of	IN
228	231	two	two	CD
232	241	promoters	promoter	NNS
241	242	.	.	.

243	245	We	we	PRP
246	252	report	report	VBP
253	256	the	the	DT
257	273	characterization	characterization	NN
274	276	of	of	IN
277	280	the	the	DT
281	285	cis-	cis-	JJ
286	289	and	and	CC
290	302	trans-acting	trans-acting	JJ
303	311	elements	element	NNS
312	320	involved	involve	VBN
321	323	in	in	IN
324	327	the	the	DT
328	343	tissue-specific	tissue-specific	JJ
344	352	activity	activity	NN
353	355	of	of	IN
356	359	the	the	DT
360	361	L	L	NNP
362	366	gene	gene	NN
367	376	erythroid	erythroid	JJ
377	385	promoter	promoter	NN
385	386	.	.	.

387	388	A	a	DT
389	393	R-PK	R-PK	NNP
394	397	DNA	DNA	NNP
398	406	fragment	fragment	NN
407	416	extending	extend	VBG
417	421	from	from	IN
422	426	-870	-870	CD
427	429	to	to	TO
430	433	+54	+54	CD
434	442	relative	relative	JJ
443	445	to	to	TO
446	449	the	the	DT
450	453	cap	cap	NN
454	458	site	site	NN
459	466	confers	confer	VBZ
467	476	erythroid	erythroid	JJ
477	488	specificity	specificity	NN
489	491	to	to	TO
492	493	a	a	DT
494	502	reporter	reporter	NN
503	507	gene	gene	NN
507	508	.	.	.

509	515	Within	within	IN
516	520	this	this	DT
521	527	region	region	NN
527	528	,	,	,
529	531	we	we	PRP
532	538	define	define	VBP
539	540	a	a	DT
541	548	minimal	minimal	JJ
549	557	promoter	promoter	NN
558	559	(	(	(
559	562	-62	-62	CD
563	565	to	to	TO
566	569	+54	+54	CD
569	570	)	)	)
571	575	that	that	WDT
576	584	displays	display	VBZ
585	603	erythroid-specific	erythroid-specific	JJ
604	612	activity	activity	NN
613	616	and	and	CC
617	625	contains	contain	VBZ
626	629	two	two	CD
630	633	DNA	dna	NN
634	641	binding	binding	NN
642	647	sites	site	NNS
647	648	.	.	.

649	652	One	one	CD
652	653	,	,	,
654	661	located	located	JJ
662	664	at	at	IN
665	668	-50	-50	CD
668	669	,	,	,
670	675	binds	bind	VBZ
676	683	members	member	NNS
684	686	of	of	IN
687	690	the	the	DT
691	700	CCACC/Sp1	ccacc/sp1	NN
701	707	family	family	NN
708	711	and	and	CC
712	715	the	the	DT
716	721	other	other	JJ
721	722	,	,	,
723	730	located	located	JJ
731	733	at	at	IN
734	737	-20	-20	CD
737	738	,	,	,
739	744	binds	bind	VBZ
745	748	the	the	DT
749	758	erythroid	erythroid	JJ
759	765	factor	factor	NN
766	772	GATA-1	gata-1	NN
772	773	.	.	.

774	782	Although	although	IN
783	786	the	the	DT
787	790	-20	-20	CD
791	795	GATA	GATA	NNP
796	803	binding	binding	NN
804	808	site	site	NN
809	810	(	(	(
810	816	AGATAA	AGATAA	NNP
816	817	)	)	)
818	820	is	be	VBZ
821	825	also	also	RB
826	827	a	a	DT
828	837	potential	potential	JJ
838	843	TFIID	TFIID	NNP
844	851	binding	binding	NN
852	856	site	site	NN
856	857	,	,	,
858	860	it	it	PRP
861	865	does	do	VBZ
866	869	not	not	RB
870	874	bind	bind	VB
875	880	TFIID	tfiid	NN
880	881	.	.	.

882	893	Furthermore	furthermore	RB
893	894	,	,	,
895	898	the	the	DT
899	911	substitution	substitution	NN
912	914	of	of	IN
915	919	this	this	DT
920	924	GATA	GATA	NNP
925	932	binding	binding	NN
933	937	site	site	NN
938	940	by	by	IN
941	942	a	a	DT
943	952	canonical	canonical	JJ
953	958	TFIID	TFIID	NNP
959	966	binding	binding	NN
967	971	site	site	NN
972	982	suppresses	suppress	VBZ
983	986	the	the	DT
987	995	promoter	promoter	NN
996	1004	activity	activity	NN
1004	1005	.	.	.

1006	1015	Mutations	mutation	NNS
1016	1019	and	and	CC
1020	1029	deletions	deletion	NNS
1030	1032	of	of	IN
1033	1037	both	both	DT
1038	1043	sites	site	NNS
1044	1052	indicate	indicate	VBP
1053	1057	that	that	IN
1058	1062	only	only	RB
1063	1066	the	the	DT
1067	1078	association	association	NN
1079	1081	of	of	IN
1082	1091	CCACC/Sp1	CCACC/Sp1	NNP
1092	1095	and	and	CC
1096	1100	GATA	GATA	NNP
1101	1108	binding	binding	NN
1109	1114	sites	site	NNS
1115	1118	can	can	MD
1119	1124	drive	drive	VB
1125	1134	efficient	efficient	JJ
1135	1138	and	and	CC
1139	1154	tissue-specific	tissue-specific	JJ
1155	1165	expression	expression	NN
1166	1168	of	of	IN
1169	1173	this	this	DT
1174	1178	R-PK	R-PK	NNP
1179	1186	minimal	minimal	JJ
1187	1195	promoter	promoter	NN
1195	1196	.	.	.

1197	1204	Finally	finally	RB
1204	1205	,	,	,
1206	1208	by	by	IN
1209	1224	co-transfection	co-transfection	NN
1225	1236	experiments	experiment	NNS
1236	1237	,	,	,
1238	1240	we	we	PRP
1241	1246	study	study	VBP
1247	1250	the	the	DT
1251	1259	elements	element	NNS
1260	1268	involved	involve	VBN
1269	1271	in	in	IN
1272	1275	the	the	DT
1276	1283	hGATA-1	hgata-1	NN
1284	1299	transactivation	transactivation	NN
1300	1302	of	of	IN
1303	1306	the	the	DT
1307	1311	R-PK	R-PK	NNP
1312	1320	promoter	promoter	NN
1321	1323	in	in	IN
1324	1328	HeLa	HeLa	NNP
1329	1334	cells	cell	NNS
1334	1335	.	.	.