0 15 Transcriptional transcriptional JJ 16 26 regulation regulation NN 27 29 of of IN 30 41 interleukin interleukin NN 42 43 3 3 CD 44 45 ( ( ( 45 48 IL3 il3 NN 48 49 ) ) ) 50 52 in in IN 53 60 primary primary JJ 61 66 human human JJ 67 68 T t NN 69 80 lymphocytes lymphocyte NNS 80 81 . . . 82 86 Role role NN 87 89 of of IN 90 95 AP-1- ap-1- NN 96 99 and and CC 100 115 octamer-binding octamer-binding JJ 116 124 proteins protein NNS 125 127 in in IN 128 135 control control NN 136 138 of of IN 139 142 IL3 il3 NN 143 147 gene gene NN 148 158 expression expression NN 158 159 . . . 161 163 We we PRP 164 168 have have VBP 169 181 investigated investigate VBN 182 185 the the DT 186 195 molecular molecular JJ 196 199 and and CC 200 211 biochemical biochemical JJ 212 217 basis basis NN 218 221 for for IN 222 232 activation activation NN 233 235 of of IN 236 247 interleukin interleukin NN 248 249 3 3 CD 250 251 ( ( ( 251 254 IL3 il3 NN 254 255 ) ) ) 256 260 gene gene NN 261 271 expression expression NN 272 274 in in IN 275 282 primary primary JJ 283 288 human human JJ 289 290 T t NN 291 302 lymphocytes lymphocyte NNS 303 312 following follow VBG 313 316 CD3 cd3 NN 317 320 and and CC 321 324 CD2 cd2 NN 325 333 receptor receptor NN 334 345 stimulation stimulation NN 346 348 or or CC 349 359 activation activation NN 360 362 by by IN 363 381 phytohemagglutinin phytohemagglutinin NN 382 386 plus plus CC 387 394 phorbol phorbol NN 395 407 12-myristate 12-myristate NN 408 418 13-acetate 13-acetate NN 418 419 . . . 420 425 Using use VBG 426 438 transfection transfection NN 439 442 and and CC 443 451 reporter reporter NN 452 456 gene gene NN 457 463 assays assay NNS 464 476 specifically specifically RB 477 485 designed design VBN 486 489 for for IN 490 497 primary primary JJ 498 499 T t NN 500 511 lymphocytes lymphocyte NNS 512 514 in in IN 515 526 conjunction conjunction NN 527 531 with with IN 532 535 gel gel NN 536 547 retardation retardation NN 548 554 assays assay NNS 554 555 , , , 556 563 Western western JJ 564 568 blot blot NN 569 577 analyses analysis NNS 578 581 and and CC 582 584 UV UV NNP 585 598 cross-linking cross-linking JJ 599 606 studies study NNS 606 607 , , , 608 610 we we PRP 611 616 found find VBD 617 621 that that IN 622 627 c-Jun c-jun NN 627 628 , , , 629 634 c-Fos c-fos NN 634 635 , , , 636 639 and and CC 640 655 octamer-binding octamer-binding JJ 656 664 proteins protein NNS 665 669 play play VBP 670 671 a a DT 672 677 major major JJ 678 682 role role NN 683 685 in in IN 686 701 transcriptional transcriptional JJ 702 712 activation activation NN 713 715 of of IN 716 719 the the DT 720 723 IL3 il3 NN 724 728 gene gene NN 729 732 via via IN 733 738 their their PRP$ 739 750 interaction interaction NN 751 755 with with IN 756 759 two two CD 760 768 specific specific JJ 769 776 regions region NNS 777 786 contained contain VBN 787 793 within within IN 794 797 the the DT 798 801 IL3 il3 NN 802 813 5’-flanking 5'-flanking JJ 814 822 sequence sequence NN 822 823 . . . 824 836 Additionally additionally RB 836 837 , , , 838 841 the the DT 842 848 region region NN 849 856 between between IN 857 862 bases base NNS 863 867 -107 -107 CD 868 871 and and CC 872 875 -59 -59 CD 876 878 of of IN 879 882 the the DT 883 886 IL3 il3 NN 887 895 promoter promoter NN 896 906 containing contain VBG 907 915 putative putative JJ 916 920 AP-2 ap-2 NN 921 924 and and CC 925 928 Sp1 sp1 NN 929 936 binding binding NN 937 943 motifs motif NNS 944 951 appears appear VBZ 952 961 necessary necessary JJ 962 965 for for IN 966 971 basal basal JJ 972 977 level level NN 978 988 expression expression NN 989 991 of of IN 992 995 the the DT 996 999 IL3 il3 NN 1000 1004 gene gene NN 1004 1005 . . . 1006 1009 The the DT 1010 1014 data datum NNS 1015 1019 also also RB 1020 1028 indicate indicate VBP 1029 1033 that that IN 1034 1037 CD2 cd2 NN 1038 1046 receptor receptor NN 1047 1057 activation activation NN 1058 1061 and and CC 1062 1080 phytohemagglutinin phytohemagglutinin NN 1081 1085 plus plus CC 1086 1093 phorbol phorbol NN 1094 1106 12-myristate 12-myristate NN 1107 1117 13-acetate 13-acetate NN 1118 1129 stimulation stimulation NN 1130 1137 augment augment VBP 1138 1139 T t NN 1140 1144 cell cell NN 1145 1148 IL3 il3 NN 1149 1153 gene gene NN 1154 1164 expression expression NN 1165 1172 through through IN 1173 1176 the the DT 1177 1181 same same JJ 1182 1186 cis- cis- JJ 1187 1190 and and CC 1191 1207 trans-activating trans-activating JJ 1208 1215 signals signal NNS 1215 1216 . . . 1217 1222 These these DT 1223 1230 results result NNS 1231 1237 should should MD 1238 1248 contribute contribute VB 1249 1251 to to TO 1252 1253 a a DT 1254 1260 better good JJR 1261 1274 understanding understanding NN 1275 1277 of of IN 1278 1281 the the DT 1282 1292 regulation regulation NN 1293 1295 of of IN 1296 1299 IL3 il3 NN 1300 1304 gene gene NN 1305 1315 expression expression NN 1316 1318 in in IN 1319 1324 human human JJ 1325 1326 T t NN 1327 1338 lymphocytes lymphocyte NNS 1338 1339 . . .