0 10 Functional functional JJ 11 27 characterization characterization NN 28 30 of of IN 31 34 the the DT 35 41 murine murine JJ 42 49 homolog homolog NN 50 52 of of IN 53 56 the the DT 57 58 B B NNP 59 72 cell-specific cell-specific JJ 73 84 coactivator coactivator NN 85 96 BOB.1/OBF.1 bob.1/obf.1 NN 96 97 . . . 99 100 B B NNP 101 114 cell-specific cell-specific JJ 115 130 transcriptional transcriptional JJ 131 139 promoter promoter NN 140 148 activity activity NN 149 157 mediated mediate VBN 158 160 by by IN 161 164 the the DT 165 172 octamer octamer NN 173 178 motif motif NN 179 187 requires require VBZ 188 191 the the DT 192 196 Oct1 oct1 NN 197 199 or or CC 200 204 Oct2 oct2 NN 205 212 protein protein NN 213 216 and and CC 217 227 additional additional JJ 228 229 B B NNP 230 245 cell-restricted cell-restricted JJ 246 255 cofactors cofactor NNS 255 256 . . . 257 260 One one CD 261 265 such such JJ 266 274 cofactor cofactor NN 274 275 , , , 276 287 BOB.1/OBF.1 BOB.1/OBF.1 NNP 287 288 , , , 289 292 was be VBD 293 301 recently recently RB 302 310 isolated isolate VBN 311 315 from from IN 316 321 human human JJ 322 323 B B NNP 324 329 cells cell NNS 329 330 . . . 331 335 Here here RB 335 336 , , , 337 339 we we PRP 340 348 describe describe VBP 349 352 the the DT 353 362 isolation isolation NN 363 366 and and CC 367 375 detailed detailed JJ 376 392 characterization characterization NN 393 395 of of IN 396 399 the the DT 400 406 murine murine JJ 407 414 homolog homolog NN 414 415 . . . 416 427 Full-length full-length JJ 428 433 cDNAs cdna NNS 434 437 and and CC 438 445 genomic genomic JJ 446 452 clones clone NNS 453 457 were be VBD 458 466 isolated isolate VBN 466 467 , , , 468 471 and and CC 472 475 the the DT 476 480 gene gene NN 481 490 structure structure NN 491 494 was be VBD 495 505 determined determine VBN 505 506 . . . 507 517 Comparison comparison NN 518 520 of of IN 521 524 the the DT 525 532 deduced deduce VBN 533 538 amino amino NN 539 544 acids acid NNS 545 550 shows show VBZ 551 553 88 88 CD 553 554 % % NN 555 563 sequence sequence NN 564 572 identity identity NN 573 580 between between IN 581 586 mouse mouse NN 587 590 and and CC 591 596 human human JJ 597 608 BOB.1/OBF.1 bob.1/obf.1 NN 608 609 . . . 610 613 The the DT 614 626 NH2-terminal nh2-terminal JJ 627 630 126 126 CD 631 636 amino amino NN 637 642 acids acid NNS 643 645 of of IN 646 657 BOB.1/OBF.1 BOB.1/OBF.1 NNP 658 661 are be VBP 662 666 both both DT 667 676 essential essential JJ 677 680 and and CC 681 691 sufficient sufficient JJ 692 695 for for IN 696 707 interaction interaction NN 708 712 with with IN 713 716 the the DT 717 720 POU POU NNP 721 728 domains domain NNS 729 731 of of IN 732 738 either either CC 739 743 Oct1 oct1 NN 744 746 or or CC 747 751 Oct2 oct2 NN 751 752 . . . 753 757 This this DT 758 773 protein-protein protein-protein JJ 774 785 interaction interaction NN 786 790 does do VBZ 791 794 not not RB 795 802 require require VB 803 806 the the DT 807 819 simultaneous simultaneous JJ 820 827 binding binding NN 828 830 of of IN 831 834 Oct Oct NNP 835 843 proteins protein NNS 844 846 to to TO 847 850 DNA DNA NNP 850 851 , , , 852 855 and and CC 856 860 high high JJ 861 871 resolution resolution NN 872 884 footprinting footprinting NN 885 887 of of IN 888 891 the the DT 892 899 Oct-DNA oct-dna JJ 900 911 interaction interaction NN 912 919 reveals reveal VBZ 920 924 that that IN 925 932 binding binding NN 933 935 of of IN 936 947 BOB.1/OBF.1 bob.1/obf.1 NN 948 950 to to TO 951 955 Oct1 Oct1 NNP 956 958 or or CC 959 963 Oct2 Oct2 NNP 964 968 does do VBZ 969 972 not not RB 973 978 alter alter VB 979 982 the the DT 983 994 interaction interaction NN 995 999 with with IN 1000 1003 DNA DNA NNP 1003 1004 . . . 1005 1016 BOB.1/OBF.1 BOB.1/OBF.1 NNP 1017 1020 can can MD 1021 1032 efficiently efficiently RB 1033 1041 activate activate VB 1042 1059 octamer-dependent octamer-dependent JJ 1060 1069 promoters promoter NNS 1070 1072 in in IN 1073 1084 fibroblasts fibroblast NNS 1084 1085 ; ; : 1086 1093 however however RB 1093 1094 , , , 1095 1097 it it PRP 1098 1103 fails fail VBZ 1104 1106 to to TO 1107 1116 stimulate stimulate VB 1117 1134 octamer-dependent octamer-dependent JJ 1135 1143 enhancer enhancer NN 1144 1152 activity activity NN 1152 1153 . . . 1154 1160 Fusion fusion NN 1161 1163 of of IN 1164 1174 subdomains subdomain NNS 1175 1177 of of IN 1178 1189 BOB.1/OBF.1 bob.1/obf.1 NN 1190 1194 with with IN 1195 1198 the the DT 1199 1203 GAL4 GAL4 NNP 1204 1207 DNA DNA NNP 1208 1215 binding binding NN 1216 1222 domain domain NN 1223 1230 reveals reveal VBZ 1231 1235 that that IN 1236 1240 both both CC 1241 1245 NH2- NH2- NNP 1246 1249 and and CC 1250 1263 COOH-terminal cooh-terminal JJ 1264 1271 domains domain NNS 1272 1274 of of IN 1275 1286 BOB.1/OBF.1 BOB.1/OBF.1 NNP 1287 1297 contribute contribute VBP 1298 1300 to to TO 1301 1305 full full JJ 1306 1321 transactivation transactivation NN 1322 1330 function function NN 1330 1331 , , , 1332 1335 the the DT 1336 1349 COOH-terminal cooh-terminal JJ 1350 1356 domain domain NN 1357 1359 is be VBZ 1360 1364 more more RBR 1365 1374 efficient efficient JJ 1375 1377 in in IN 1378 1382 this this DT 1383 1398 transactivation transactivation NN 1399 1404 assay assay NN 1404 1405 . . . 1406 1416 Consistent consistent JJ 1417 1421 with with IN 1422 1425 the the DT 1426 1433 failure failure NN 1434 1436 of of IN 1437 1448 full-length full-length JJ 1449 1460 BOB.1/OBF.1 bob.1/obf.1 NN 1461 1463 to to TO 1464 1473 stimulate stimulate VB 1474 1491 octamer-dependent octamer-dependent JJ 1492 1500 enhancer enhancer NN 1501 1509 elements element NNS 1510 1512 in in IN 1513 1516 non non JJ 1517 1518 B B NNP 1519 1524 cells cell NNS 1524 1525 , , , 1526 1529 the the DT 1530 1534 GAL4 gal4 NN 1535 1542 fusions fusion NNS 1543 1551 likewise likewise RB 1552 1556 only only RB 1557 1566 stimulate stimulate VB 1567 1571 from from IN 1572 1573 a a DT 1574 1591 promoter-proximal promoter-proximal JJ 1592 1600 position position NN 1600 1601 . . .