0 4 Role role NN 5 7 of of IN 8 12 EGR1 egr1 NN 13 15 in in IN 16 26 regulation regulation NN 27 29 of of IN 30 48 stimulus-dependent stimulus-dependent JJ 49 53 CD44 cd44 NN 54 67 transcription transcription NN 68 70 in in IN 71 72 B B NNP 73 84 lymphocytes lymphocyte NNS 84 85 . . . 87 90 The the DT 91 106 immediate-early immediate-early JJ 107 111 gene gene NN 112 117 egr-1 egr-1 NN 118 125 encodes encode VBZ 126 127 a a DT 128 141 transcription transcription NN 142 148 factor factor NN 149 150 ( ( ( 150 154 EGR1 EGR1 NNP 154 155 ) ) ) 156 160 that that WDT 161 166 links link VBZ 167 173 B-cell b-cell NN 174 181 antigen antigen NN 182 190 receptor receptor NN 191 192 ( ( ( 192 195 BCR BCR NNP 195 196 ) ) ) 197 204 signals signal NNS 205 207 to to TO 208 218 downstream downstream JJ 219 229 activation activation NN 230 236 events event NNS 237 244 through through IN 245 248 the the DT 249 259 regulation regulation NN 260 262 of of IN 263 273 previously previously RB 274 286 unidentified unidentified JJ 287 293 target target NN 294 299 genes gene NNS 299 300 . . . 301 305 Here here RB 306 308 we we PRP 309 317 identify identify VBP 318 321 the the DT 322 326 gene gene NN 327 335 encoding encode VBG 336 339 the the DT 340 350 lymphocyte lymphocyte NN 351 357 homing homing NN 358 361 and and CC 362 371 migration migration NN 372 379 protein protein NN 380 384 CD44 cd44 NN 385 387 as as IN 388 389 a a DT 390 396 target target NN 397 399 of of IN 400 404 EGR1 egr1 NN 405 415 regulation regulation NN 416 418 in in IN 419 420 B B NNP 421 426 cells cell NNS 426 427 . . . 428 439 BCR-induced bcr-induced JJ 440 449 increases increase NNS 450 452 in in IN 453 457 CD44 CD44 NNP 458 462 mRNA mrna NN 463 473 expression expression NN 474 477 and and CC 478 491 transcription transcription NN 492 498 levels level NNS 499 502 are be VBP 503 508 shown show VBN 509 511 to to TO 512 517 occur occur VB 518 520 in in IN 521 536 EGR1-expressing egr1-expressing JJ 537 540 but but CC 541 544 not not RB 545 547 in in IN 548 561 nonexpressing nonexpressing JJ 562 571 subclones subclone NNS 572 574 of of IN 575 578 the the DT 579 585 B-cell b-cell NN 586 590 line line NN 591 599 WEHI-231 wehi-231 NN 599 600 . . . 601 609 Kinetics Kinetic NNP 610 612 of of IN 613 618 egr-1 egr-1 NN 619 632 transcription transcription NN 633 636 and and CC 637 640 the the DT 641 651 appearance appearance NN 652 654 of of IN 655 662 nuclear nuclear JJ 663 667 EGR1 egr1 NN 668 675 protein protein NN 676 683 precede precede VBP 684 688 CD44 cd44 NN 689 698 induction induction NN 699 702 and and CC 703 708 occur occur VBP 709 715 within within IN 716 718 30 30 CD 719 722 min min NN 723 728 after after IN 729 740 stimulation stimulation NN 741 743 in in IN 744 747 the the DT 748 763 EGR1-expressing egr1-expressing JJ 764 772 subclone subclone NN 772 773 . . . 774 775 A a DT 776 782 single single JJ 783 787 EGR1 egr1 NN 788 795 binding binding NN 796 801 motif motif NN 802 804 is be VBZ 805 817 demonstrated demonstrate VBN 818 820 at at IN 821 823 bp bp NN 824 828 -301 -301 CD 829 831 of of IN 832 835 the the DT 836 841 human human JJ 842 846 CD44 cd44 NN 847 855 promoter promoter NN 855 856 . . . 857 871 Cotransfection cotransfection NN 872 874 of of IN 875 876 a a DT 877 881 CD44 cd44 NN 882 906 promoter-chloramphenicol promoter-chloramphenicol JJ 907 924 acetyltransferase acetyltransferase NN 925 933 reporter reporter NN 934 943 construct construct NN 944 948 with with IN 949 951 an an DT 952 957 egr-1 egr-1 NN 958 968 expression expression NN 969 975 vector vector NN 976 984 resulted result VBD 985 987 in in IN 988 989 a a DT 990 994 6.5- 6.5- CD 995 997 to to TO 998 1006 8.5-fold 8.5-fold JJ 1007 1016 induction induction NN 1017 1019 of of IN 1020 1035 transcriptional transcriptional JJ 1036 1044 activity activity NN 1045 1053 relative relative JJ 1054 1056 to to TO 1057 1059 an an DT 1060 1065 empty empty JJ 1066 1076 expression expression NN 1077 1083 vector vector NN 1083 1084 . . . 1085 1088 The the DT 1089 1093 EGR1 egr1 NN 1094 1101 binding binding NN 1102 1107 motif motif NN 1108 1111 was be VBD 1112 1117 shown show VBN 1118 1120 to to TO 1121 1123 be be VB 1124 1133 necessary necessary JJ 1134 1137 for for IN 1138 1154 stimulus-induced stimulus-induced JJ 1155 1165 expression expression NN 1166 1168 of of IN 1169 1170 a a DT 1171 1175 CD44 cd44 NN 1176 1200 promoter-chloramphenicol promoter-chloramphenicol JJ 1201 1218 acetyltransferase acetyltransferase NN 1219 1227 reporter reporter NN 1228 1237 construct construct NN 1238 1240 in in IN 1241 1255 nontransformed nontransformed JJ 1256 1257 B B NNP 1258 1269 lymphocytes lymphocyte NNS 1270 1273 and and CC 1274 1277 was be VBD 1278 1286 required require VBN 1287 1290 for for IN 1291 1306 transactivation transactivation NN 1307 1309 by by IN 1310 1312 an an DT 1313 1317 EGR1 egr1 NN 1318 1328 expression expression NN 1329 1335 vector vector NN 1336 1338 in in IN 1339 1340 a a DT 1341 1347 B-cell b-cell NN 1348 1352 line line NN 1352 1353 . . . 1354 1359 These these DT 1360 1367 studies study NNS 1368 1376 identify identify VBP 1377 1381 EGR1 egr1 NN 1382 1384 as as IN 1385 1387 an an DT 1388 1400 intermediary intermediary JJ 1401 1408 linking link VBG 1409 1420 BCR-derived bcr-derived JJ 1421 1428 signals signal NNS 1429 1431 to to TO 1432 1435 the the DT 1436 1445 induction induction NN 1446 1448 of of IN 1449 1453 CD44 cd44 NN 1453 1454 . . . 1455 1458 The the DT 1459 1468 relevance relevance NN 1469 1471 of of IN 1472 1477 these these DT 1478 1487 molecular molecular JJ 1488 1494 events event NNS 1495 1497 to to TO 1498 1501 BCR BCR NNP 1502 1508 signal signal NN 1509 1521 transduction transduction NN 1522 1525 and and CC 1526 1544 antigen-stimulated antigen-stimulated JJ 1545 1560 B-cell-mediated b-cell-mediated JJ 1561 1567 immune immune JJ 1568 1577 responses response NNS 1578 1580 is be VBZ 1581 1590 discussed discuss VBN 1590 1591 . . .