0 14 Identification identification NN 15 17 of of IN 18 20 an an DT 21 30 inducible inducible JJ 31 40 regulator regulator NN 41 43 of of IN 44 49 c-myb c-myb NN 50 60 expression expression NN 61 67 during during IN 68 74 T-cell t-cell NN 75 85 activation activation NN 85 86 . . . 88 95 Resting rest VBG 96 97 T t NN 98 103 cells cell NNS 104 111 express express VBP 112 116 very very RB 117 120 low low JJ 121 127 levels level NNS 128 130 of of IN 131 136 c-Myb c-myb NN 137 144 protein protein NN 144 145 . . . 146 152 During during IN 153 159 T-cell t-cell NN 160 170 activation activation NN 170 171 , , , 172 177 c-myb c-myb NN 178 188 expression expression NN 189 191 is be VBZ 192 199 induced induce VBN 200 203 and and CC 204 208 much much JJ 209 211 of of IN 212 215 the the DT 216 224 increase increase NN 225 227 in in IN 228 238 expression expression NN 239 245 occurs occur VBZ 246 248 at at IN 249 252 the the DT 253 268 transcriptional transcriptional JJ 269 274 level level NN 274 275 . . . 276 278 We we PRP 279 289 identified identify VBD 290 291 a a DT 292 298 region region NN 299 301 of of IN 302 305 the the DT 306 311 c-myb c-myb NN 312 313 5 5 CD 313 314 ’ ' SYM 315 323 flanking flanking NN 324 332 sequence sequence NN 333 337 that that WDT 338 347 increased increase VBD 348 353 c-myb c-myb NN 354 364 expression expression NN 365 371 during during IN 372 378 T-cell t-cell NN 379 389 activation activation NN 389 390 . . . 391 393 In in FW 394 398 vivo vivo FW 399 411 footprinting footprinting NN 412 414 by by IN 415 432 ligation-mediated ligation-mediated JJ 433 436 PCR pcr NN 437 440 was be VBD 441 450 performed perform VBN 451 453 to to TO 454 463 correlate correlate VB 464 466 in in FW 467 471 vivo vivo FW 472 479 protein protein NN 480 487 binding binding NN 488 492 with with IN 493 503 functional functional JJ 504 512 activity activity NN 512 513 . . . 514 515 A a DT 516 523 protein protein NN 524 533 footprint footprint NN 534 537 was be VBD 538 545 visible visible JJ 546 550 over over IN 551 555 this this DT 556 562 region region NN 563 565 of of IN 566 569 the the DT 570 575 c-myb c-myb NN 576 577 5 5 CD 577 578 ’ ' SYM 579 587 flanking flanking NN 588 596 sequence sequence NN 597 599 in in IN 600 609 activated activate VBN 610 611 T t NN 612 617 cells cell NNS 618 621 but but CC 622 625 not not RB 626 628 in in IN 629 640 unactivated unactivated JJ 641 642 T t NN 643 648 cells cell NNS 648 649 . . . 650 652 An an DT 653 668 electrophoretic electrophoretic JJ 669 677 mobility mobility NN 678 683 shift shift NN 684 689 assay assay NN 690 691 ( ( ( 691 695 EMSA EMSA NNP 695 696 ) ) ) 697 701 with with IN 702 709 nuclear nuclear JJ 710 717 extract extract NN 718 722 from from IN 723 732 activated activate VBN 733 734 T t NN 735 740 cells cell NNS 741 744 and and CC 745 747 an an DT 748 763 oligonucleotide oligonucleotide NN 764 766 of of IN 767 771 this this DT 772 779 binding bind VBG 780 784 site site NN 785 797 demonstrated demonstrate VBD 798 799 a a DT 800 803 new new JJ 804 815 protein-DNA protein-dna JJ 816 823 complex complex NN 823 824 , , , 825 833 referred refer VBN 834 836 to to TO 837 839 as as IN 840 844 CMAT CMAT NNP 845 848 for for IN 849 854 c-myb c-myb NN 855 857 in in IN 858 867 activated activate VBN 868 869 T t NN 870 875 cells cell NNS 875 876 ; ; : 877 881 this this DT 882 889 complex complex NN 890 893 was be VBD 894 897 not not RB 898 905 present present JJ 906 908 in in IN 909 920 unactivated unactivated JJ 921 922 T t NN 923 928 cells cell NNS 928 929 . . . 930 937 Because because IN 938 941 the the DT 942 949 binding bind VBG 950 954 site site NN 955 961 showed show VBD 962 966 some some DT 967 975 sequence sequence NN 976 986 similarity similarity NN 987 991 with with IN 992 995 the the DT 996 1003 nuclear nuclear JJ 1004 1010 factor factor NN 1011 1013 of of IN 1014 1023 activated activate VBN 1024 1025 T t NN 1026 1031 cells cell NNS 1032 1033 ( ( ( 1033 1037 NFAT NFAT NNP 1037 1038 ) ) ) 1039 1046 binding binding NN 1047 1051 site site NN 1051 1052 , , , 1053 1055 we we PRP 1056 1064 compared compare VBD 1065 1068 the the DT 1069 1077 kinetics kinetic NNS 1078 1080 of of IN 1081 1090 induction induction NN 1091 1093 of of IN 1094 1097 the the DT 1098 1101 two two CD 1102 1109 binding bind VBG 1110 1119 complexes complex NNS 1120 1123 and and CC 1124 1127 the the DT 1128 1137 molecular molecular JJ 1138 1144 masses mass NNS 1145 1147 of of IN 1148 1151 the the DT 1152 1155 two two CD 1156 1164 proteins protein NNS 1164 1165 . . . 1166 1173 Studies study NNS 1174 1176 of of IN 1177 1180 the the DT 1181 1189 kinetics kinetic NNS 1190 1192 of of IN 1193 1202 induction induction NN 1203 1209 showed show VBD 1210 1214 that that IN 1215 1218 the the DT 1219 1223 NFAT NFAT NNP 1224 1228 EMSA EMSA NNP 1229 1236 binding binding NN 1237 1244 complex complex NN 1245 1253 appeared appear VBD 1254 1261 earlier earlier RBR 1262 1266 than than IN 1267 1270 the the DT 1271 1275 CMAT CMAT NNP 1276 1283 complex complex NN 1283 1284 . . . 1285 1288 The the DT 1289 1293 NFAT NFAT NNP 1294 1301 protein protein NN 1302 1310 migrated migrate VBD 1311 1315 more more RBR 1316 1322 slowly slowly RB 1323 1325 in in IN 1326 1327 a a DT 1328 1334 sodium sodium NN 1335 1342 dodecyl dodecyl NN 1343 1365 sulfate-polyacrylamide sulfate-polyacrylamide JJ 1366 1369 gel gel NN 1370 1374 than than IN 1375 1378 the the DT 1379 1383 CMAT CMAT NNP 1384 1391 protein protein NN 1392 1395 did do VBD 1395 1396 . . . 1397 1399 In in IN 1400 1408 addition addition NN 1408 1409 , , , 1410 1412 an an DT 1413 1421 antibody antibody NN 1422 1429 against against IN 1430 1434 NFAT NFAT NNP 1435 1438 did do VBD 1439 1442 not not RB 1443 1454 cross-react cross-react VB 1455 1459 with with IN 1460 1463 the the DT 1464 1468 CMAT CMAT NNP 1469 1476 protein protein NN 1476 1477 . . . 1478 1481 The the DT 1482 1492 appearance appearance NN 1493 1495 of of IN 1496 1499 the the DT 1500 1504 CMAT CMAT NNP 1505 1512 binding binding NN 1513 1520 complex complex NN 1521 1524 was be VBD 1525 1534 inhibited inhibit VBN 1535 1537 by by IN 1538 1542 both both CC 1543 1554 cyclosporin cyclosporin NN 1555 1556 A a NN 1557 1560 and and CC 1561 1570 rapamycin rapamycin NN 1570 1571 . . . 1572 1575 The the DT 1576 1580 CMAT CMAT NNP 1581 1588 protein protein NN 1589 1596 appears appear VBZ 1597 1599 to to TO 1600 1602 be be VB 1603 1604 a a DT 1605 1610 novel novel JJ 1611 1620 inducible inducible JJ 1621 1628 protein protein NN 1629 1637 involved involve VBN 1638 1640 in in IN 1641 1644 the the DT 1645 1655 regulation regulation NN 1656 1658 of of IN 1659 1664 c-myb c-myb NN 1665 1675 expression expression NN 1676 1682 during during IN 1683 1689 T-cell t-cell NN 1690 1700 activation activation NN 1700 1701 . . .