0 10 Structural structural JJ 11 14 and and CC 15 25 functional functional JJ 26 42 characterization characterization NN 43 45 of of IN 46 49 the the DT 50 55 human human JJ 56 60 CD36 cd36 NN 61 65 gene gene NN 66 74 promoter promoter NN 74 75 : : : 76 90 identification identification NN 91 93 of of IN 94 95 a a DT 96 104 proximal proximal JJ 105 114 PEBP2/CBF pebp2/cbf NN 115 119 site site NN 119 120 . . . 122 126 CD36 CD36 NNP 127 129 is be VBZ 130 131 a a DT 132 136 cell cell NN 137 144 surface surface NN 145 157 glycoprotein glycoprotein NN 158 166 composed compose VBN 167 169 of of IN 170 171 a a DT 172 178 single single JJ 179 190 polypeptide polypeptide NN 191 196 chain chain NN 196 197 , , , 198 203 which which WDT 204 213 interacts interact VBZ 214 218 with with IN 219 233 thrombospondin thrombospondin NN 233 234 , , , 235 244 collagens collagen NNS 245 249 type type NN 250 251 I i CD 252 255 and and CC 256 258 IV iv CD 258 259 , , , 260 268 oxidized oxidize VBN 269 272 low low JJ 273 280 density density NN 281 292 lipoprotein lipoprotein NN 292 293 , , , 294 299 fatty fatty JJ 300 305 acids acid NNS 305 306 , , , 307 314 anionic anionic JJ 315 328 phospholipids phospholipid NNS 328 329 , , , 330 333 and and CC 334 346 erythrocytes erythrocyte NNS 347 358 parasitized parasitize VBN 359 363 with with IN 364 374 Plasmodium Plasmodium NNP 375 385 falciparum falciparum NNP 385 386 . . . 387 390 Its its PRP$ 391 401 expression expression NN 402 404 is be VBZ 405 415 restricted restricted JJ 416 418 to to TO 419 420 a a DT 421 424 few few JJ 425 429 cell cell NN 430 435 types type NNS 435 436 , , , 437 446 including include VBG 447 467 monocyte/macrophages monocyte/macrophage NNS 467 468 . . . 469 471 In in IN 472 477 these these DT 478 483 cells cell NNS 483 484 , , , 485 489 CD36 CD36 NNP 490 492 is be VBZ 493 501 involved involve VBN 502 504 in in IN 505 517 phagocytosis phagocytosis NN 518 520 of of IN 521 530 apoptotic apoptotic JJ 531 536 cells cell NNS 536 537 , , , 538 541 and and CC 542 546 foam foam NN 547 551 cell cell NN 552 561 formation formation NN 562 564 by by IN 565 571 uptake uptake NN 572 574 of of IN 575 583 oxidized oxidize VBN 584 587 low low JJ 588 595 density density NN 596 607 lipoprotein lipoprotein NN 607 608 . . . 609 611 To to TO 612 617 study study VB 618 621 the the DT 622 631 molecular molecular JJ 632 642 mechanisms mechanism NNS 643 647 that that WDT 648 655 control control VBP 656 659 the the DT 660 673 transcription transcription NN 674 676 of of IN 677 680 the the DT 681 685 CD36 CD36 NNP 686 690 gene gene NN 691 693 in in IN 694 703 monocytic monocytic JJ 704 709 cells cell NNS 710 712 we we PRP 713 717 have have VBP 718 726 isolated isolate VBN 727 730 and and CC 731 739 analyzed analyze VBN 740 743 the the DT 744 748 CD36 cd36 NN 749 757 promoter promoter NN 757 758 . . . 759 768 Transient transient JJ 769 779 expression expression NN 780 791 experiments experiment NNS 792 794 of of IN 795 806 5’-deletion 5'-deletion JJ 807 816 fragments fragment NNS 817 819 of of IN 820 823 the the DT 824 828 CD36 CD36 NNP 829 837 promoter promoter NN 838 845 coupled couple VBN 846 848 to to TO 849 859 luciferase luciferase NN 860 872 demonstrated demonstrate VBD 873 877 that that IN 878 880 as as RB 881 884 few few JJ 885 887 as as IN 888 891 158 158 CD 892 896 base base NN 897 902 pairs pair NNS 903 911 upstream upstream JJ 912 916 from from IN 917 920 the the DT 921 934 transcription transcription NN 935 945 initiation initiation NN 946 950 site site NN 951 955 were be VBD 956 966 sufficient sufficient JJ 967 969 to to TO 970 976 direct direct VB 977 980 the the DT 981 998 monocyte-specific monocyte-specific JJ 999 1012 transcription transcription NN 1013 1015 of of IN 1016 1019 the the DT 1020 1028 reporter reporter NN 1029 1033 gene gene NN 1033 1034 . . . 1035 1041 Within within IN 1042 1045 the the DT 1046 1051 above above JJ 1052 1058 region region NN 1058 1059 , , , 1060 1063 the the DT 1064 1072 fragment fragment NN 1073 1081 spanning span VBG 1082 1093 nucleotides nucleotide NNS 1094 1098 -158 -158 CD 1099 1101 to to TO 1102 1105 -90 -90 CD 1106 1109 was be VBD 1110 1118 required require VBN 1119 1122 for for IN 1123 1130 optimal optimal JJ 1131 1144 transcription transcription NN 1145 1147 in in IN 1148 1157 monocytic monocytic JJ 1158 1163 cells cell NNS 1163 1164 . . . 1165 1176 Biochemical biochemical JJ 1177 1185 analysis analysis NN 1186 1188 of of IN 1189 1192 the the DT 1193 1199 region region NN 1200 1208 -158/-90 -158/-90 CD 1209 1217 revealed reveal VBD 1218 1219 a a DT 1220 1227 binding bind VBG 1228 1232 site site NN 1233 1236 for for IN 1237 1250 transcription transcription NN 1251 1258 factors factor NNS 1259 1261 of of IN 1262 1265 the the DT 1266 1278 polyomavirus polyomavirus NN 1279 1295 enhancer-binding enhancer-binding JJ 1296 1303 protein protein NN 1304 1318 2/core-binding 2/core-binding JJ 1319 1325 factor factor NN 1326 1327 ( ( ( 1327 1336 PEBP2/CBF PEBP2/CBF NNP 1336 1337 ) ) ) 1338 1344 family family NN 1345 1347 at at IN 1348 1356 position position NN 1357 1361 -103 -103 CD 1361 1362 . . . 1363 1373 Disruption disruption NN 1374 1376 of of IN 1377 1380 the the DT 1381 1390 PEBP2/CBF PEBP2/CBF NNP 1391 1395 site site NN 1396 1404 markedly markedly RB 1405 1415 diminished diminish VBD 1416 1419 the the DT 1420 1424 role role NN 1425 1427 of of IN 1428 1431 the the DT 1432 1441 PEBP2/CBF PEBP2/CBF NNP 1442 1449 factors factor NNS 1450 1452 in in IN 1453 1456 the the DT 1457 1469 constitutive constitutive JJ 1470 1483 transcription transcription NN 1484 1486 of of IN 1487 1490 the the DT 1491 1495 CD36 CD36 NNP 1496 1500 gene gene NN 1500 1501 . . . 1502 1505 The the DT 1506 1517 involvement involvement NN 1518 1520 of of IN 1521 1528 members member NNS 1529 1531 of of IN 1532 1535 the the DT 1536 1545 PEBP2/CBF PEBP2/CBF NNP 1546 1552 family family NN 1553 1555 in in IN 1556 1566 chromosome chromosome NN 1567 1581 translocations translocation NNS 1582 1592 associated associate VBN 1593 1597 with with IN 1598 1603 acute acute JJ 1604 1611 myeloid myeloid JJ 1612 1620 leukemia leukemia NN 1620 1621 , , , 1622 1625 and and CC 1626 1628 in in IN 1629 1632 the the DT 1633 1648 transcriptional transcriptional JJ 1649 1659 regulation regulation NN 1660 1662 of of IN 1663 1666 the the DT 1667 1683 myeloid-specific myeloid-specific JJ 1684 1689 genes gene NNS 1690 1698 encoding encode VBG 1699 1702 for for IN 1703 1718 myeloperoxidase myeloperoxidase NN 1718 1719 , , , 1720 1728 elastase elastase NN 1728 1729 , , , 1730 1733 and and CC 1734 1737 the the DT 1738 1756 colony-stimulating colony-stimulating JJ 1757 1763 factor factor NN 1764 1772 receptor receptor NN 1772 1773 , , , 1774 1784 highlights highlight VBZ 1785 1788 the the DT 1789 1798 relevance relevance NN 1799 1801 of of IN 1802 1805 the the DT 1806 1816 regulation regulation NN 1817 1819 of of IN 1820 1823 the the DT 1824 1828 CD36 CD36 NNP 1829 1833 gene gene NN 1834 1842 promoter promoter NN 1843 1845 in in IN 1846 1855 monocytic monocytic JJ 1856 1861 cells cell NNS 1862 1864 by by IN 1865 1872 members member NNS 1873 1875 of of IN 1876 1879 the the DT 1880 1889 PEBP2/CBF PEBP2/CBF NNP 1890 1896 family family NN 1896 1897 . . .