0 16 Interferon-gamma Interferon-gamma NNP 17 26 modulates modulate VBZ 27 30 the the DT 31 57 lipopolysaccharide-induced lipopolysaccharide-induced JJ 58 68 expression expression NN 69 71 of of IN 72 76 AP-1 AP-1 NNP 77 80 and and CC 81 89 NF-kappa NF-kappa NNP 90 91 B B NNP 92 94 at at IN 95 98 the the DT 99 103 mRNA mrna NN 104 107 and and CC 108 115 protein protein NN 116 121 level level NN 122 124 in in IN 125 130 human human JJ 131 140 monocytes monocyte NNS 140 141 . . . 143 159 Interferon-gamma Interferon-gamma NNP 160 161 ( ( ( 161 170 IFN-gamma IFN-gamma NNP 170 171 ) ) ) 172 181 modulates modulate VBZ 182 185 the the DT 186 196 expression expression NN 197 199 of of IN 200 207 several several JJ 208 217 cytokines cytokine NNS 218 220 by by IN 221 226 human human JJ 227 236 monocytes monocyte NNS 237 239 at at IN 240 243 the the DT 244 259 transcriptional transcriptional JJ 260 265 level level NN 265 266 . . . 267 269 In in IN 270 274 view view NN 275 277 of of IN 278 283 these these DT 284 292 findings finding NNS 292 293 , , , 294 296 we we PRP 297 305 analyzed analyze VBD 306 309 the the DT 310 317 effects effect NNS 318 320 of of IN 321 330 IFN-gamma IFN-gamma NNP 331 333 on on IN 334 337 the the DT 338 348 expression expression NN 349 351 of of IN 352 361 different different JJ 362 375 transcription transcription NN 376 383 factors factor NNS 384 386 in in IN 387 396 activated activate VBN 397 402 human human JJ 403 412 monocytes monocyte NNS 412 413 . . . 414 421 Priming priming NN 422 424 of of IN 425 430 human human JJ 431 440 monocytes monocyte NNS 441 445 with with IN 446 455 IFN-gamma IFN-gamma NNP 456 464 resulted result VBD 465 467 in in IN 468 471 the the DT 472 476 down down JJ 477 487 regulation regulation NN 488 490 of of IN 491 496 c-fos c-fos NN 497 500 and and CC 501 506 c-jun c-jun NN 507 511 mRNA mrna NN 512 514 in in IN 515 523 response response NN 524 526 to to TO 527 538 stimulation stimulation NN 539 543 with with IN 544 562 lipopolysaccharide lipopolysaccharide NN 563 564 ( ( ( 564 567 LPS lps NN 567 568 ) ) ) 569 577 compared compare VBN 578 580 to to TO 581 584 the the DT 585 592 effects effect NNS 593 595 of of IN 596 599 LPS lps NN 600 605 alone alone RB 605 606 . . . 607 610 Not not RB 611 615 only only RB 616 619 was be VBD 620 624 this this DT 625 631 effect effect NN 632 640 observed observe VBN 641 643 at at IN 644 647 the the DT 648 652 mRNA mrna NN 653 658 level level NN 658 659 , , , 660 663 but but CC 664 673 activator activator NN 674 683 protein-1 protein-1 NN 684 685 ( ( ( 685 689 AP-1 ap-1 NN 689 690 ) ) ) 691 694 DNA DNA NNP 695 702 binding binding NN 703 711 capacity capacity NN 712 715 was be VBD 716 724 affected affect VBN 725 727 as as RB 728 732 well well RB 732 733 , , , 734 735 A a DT 736 742 strong strong JJ 743 752 reduction reduction NN 753 756 was be VBD 757 765 observed observe VBN 766 768 in in IN 769 772 the the DT 773 784 LPS-induced lps-induced JJ 785 796 DNA-binding dna-binding JJ 797 805 activity activity NN 806 808 of of IN 809 813 AP-1 ap-1 NN 814 816 in in IN 817 820 the the DT 821 829 presence presence NN 830 832 of of IN 833 842 IFN-gamma IFN-gamma NNP 842 843 . . . 844 858 LPS-stimulated lps-stimulated JJ 859 868 monocytes monocyte NNS 869 875 showed show VBD 876 878 an an DT 879 888 increased increase VBN 889 899 expression expression NN 900 902 of of IN 903 907 p105 p105 NN 908 912 mRNA mRNA NNP 912 913 , , , 914 917 the the DT 918 927 precursor precursor NN 928 930 of of IN 931 934 the the DT 935 938 p50 p50 NN 939 946 subunit subunit NN 947 949 of of IN 950 953 the the DT 954 967 transcription transcription NN 968 974 factor factor NN 975 982 nuclear nuclear JJ 983 995 factor-kappa factor-kappa NN 996 997 B B NNP 998 999 ( ( ( 999 1007 NF-kappa NF-kappa NNP 1008 1009 B B NNP 1009 1010 ) ) ) 1010 1011 , , , 1012 1017 while while IN 1018 1020 no no DT 1021 1027 effect effect NN 1028 1031 was be VBD 1032 1039 noticed notice VBN 1040 1042 on on IN 1043 1046 the the DT 1047 1057 expression expression NN 1058 1060 of of IN 1061 1064 p65 p65 NN 1065 1069 mRNA mrna NN 1069 1070 . . . 1071 1073 In in IN 1074 1082 contrast contrast NN 1082 1083 , , , 1084 1093 IFN-gamma IFN-gamma NNP 1094 1101 priming priming NN 1102 1105 did do VBD 1106 1109 not not RB 1110 1116 affect affect VB 1117 1120 the the DT 1121 1131 expression expression NN 1132 1134 of of IN 1135 1139 p105 p105 NN 1140 1151 transcripts transcript NNS 1152 1155 but but CC 1156 1164 enhanced enhance VBD 1165 1168 the the DT 1169 1179 expression expression NN 1180 1182 of of IN 1183 1186 p65 p65 NN 1187 1191 mRNA mrna NN 1192 1193 ( ( ( 1193 1201 two-fold two-fold JJ 1201 1202 ) ) ) 1202 1203 . . . 1204 1211 Priming prim VBG 1212 1216 with with IN 1217 1226 IFN-gamma IFN-gamma NNP 1227 1235 followed follow VBN 1236 1238 by by IN 1239 1242 LPS LPS NNP 1243 1254 stimulation stimulation NN 1255 1263 resulted result VBD 1264 1266 in in IN 1267 1268 a a DT 1269 1276 further further JJ 1277 1285 increase increase NN 1286 1288 in in IN 1289 1292 the the DT 1293 1303 expression expression NN 1304 1306 of of IN 1307 1310 p65 p65 NN 1311 1315 mRNA mrna NN 1315 1316 . . . 1317 1321 This this DT 1322 1325 was be VBD 1326 1329 due due JJ 1330 1332 to to TO 1333 1335 an an DT 1336 1344 increase increase NN 1345 1347 in in IN 1348 1351 the the DT 1352 1361 half-life half-life NN 1362 1364 of of IN 1365 1368 p65 p65 NN 1369 1373 mRNA mrna NN 1374 1375 ( ( ( 1375 1377 75 75 CD 1378 1380 vs vs IN 1381 1384 150 150 CD 1385 1392 minutes minute NNS 1392 1393 ) ) ) 1393 1394 . . . 1395 1410 Electrophoretic electrophoretic JJ 1411 1419 mobility mobility NN 1420 1425 shift shift NN 1426 1432 assays assay NNS 1433 1434 ( ( ( 1434 1439 EMSAs EMSA NNP 1439 1440 ) ) ) 1441 1453 demonstrated demonstrate VBD 1454 1458 that that IN 1459 1471 unstimulated unstimulated JJ 1472 1481 monocytes monocyte NNS 1482 1495 predominantly predominantly RB 1496 1505 expressed express VBD 1506 1509 p50 p50 NN 1510 1518 NF-kappa NF-kappa NNP 1519 1521 B. B. NNP 1522 1533 Stimulation Stimulation NNP 1534 1538 with with IN 1539 1542 LPS LPS NNP 1543 1545 or or CC 1546 1555 IFN-gamma IFN-gamma NNP 1556 1564 resulted result VBD 1565 1567 in in IN 1568 1571 the the DT 1572 1582 expression expression NN 1583 1585 of of IN 1586 1589 p50 p50 NN 1590 1593 and and CC 1594 1597 p65 p65 NN 1598 1606 subunits subunit NNS 1606 1607 , , , 1608 1613 while while IN 1614 1617 the the DT 1618 1629 combination combination NN 1630 1632 of of IN 1633 1642 IFN-gamma IFN-gamma NNP 1643 1647 plus plus CC 1648 1651 LPS LPS NNP 1652 1658 caused cause VBD 1659 1660 a a DT 1661 1668 further further JJ 1669 1677 increase increase NN 1678 1680 in in IN 1681 1684 the the DT 1685 1695 expression expression NN 1696 1698 of of IN 1699 1707 NF-kappa NF-kappa NNP 1708 1710 B. B. NNP 1711 1715 With with IN 1716 1723 Western Western NNP 1724 1732 blotting blotting NN 1732 1733 , , , 1734 1736 it it PRP 1737 1740 was be VBD 1741 1746 shown show VBN 1747 1751 that that IN 1752 1759 nuclear nuclear JJ 1760 1768 extracts extract NNS 1769 1773 from from IN 1774 1783 monocytes monocyte NNS 1784 1793 contained contain VBD 1794 1797 p50 p50 NN 1798 1801 and and CC 1802 1805 p65 p65 NN 1806 1813 protein protein NN 1814 1816 in in IN 1817 1825 response response NN 1826 1828 to to TO 1829 1832 LPS LPS NNP 1833 1836 and and CC 1837 1846 IFN-gamma IFN-gamma NNP 1847 1858 stimulation stimulation NN 1858 1859 . . . 1860 1867 However however RB 1867 1868 , , , 1869 1872 the the DT 1873 1881 combined combined JJ 1882 1893 stimulation stimulation NN 1894 1897 did do VBD 1898 1901 not not RB 1902 1908 result result VB 1909 1911 in in IN 1912 1920 enhanced enhance VBN 1921 1924 p50 p50 NN 1925 1928 and and CC 1929 1932 p65 p65 NN 1933 1940 protein protein NN 1941 1951 expression expression NN 1951 1952 . . . 1953 1956 The the DT 1957 1964 effects effect NNS 1965 1967 of of IN 1968 1977 IFN-gamma IFN-gamma NNP 1978 1980 on on IN 1981 1984 the the DT 1985 1998 transcription transcription NN 1999 2006 factors factor NNS 2007 2011 were be VBD 2012 2020 specific specific JJ 2020 2021 , , , 2022 2027 since since IN 2028 2030 no no DT 2031 2037 change change NN 2038 2041 was be VBD 2042 2050 observed observe VBN 2051 2053 in in IN 2054 2057 the the DT 2058 2068 expression expression NN 2069 2071 of of IN 2072 2079 NF-IL-6 NF-IL-6 NNP 2080 2082 or or CC 2083 2084 I i NN 2085 2090 kappa kappa NN 2091 2092 B B NNP 2093 2098 alpha alpha NN 2098 2099 , , , 2100 2103 the the DT 2104 2113 inhibitor inhibitor NN 2114 2116 of of IN 2117 2125 NF-kappa NF-kappa NNP 2126 2128 B. B. NNP 2129 2131 We we PRP 2132 2140 conclude conclude VBP 2141 2145 that that IN 2146 2149 the the DT 2150 2157 effects effect NNS 2158 2160 of of IN 2161 2170 IFN-gamma IFN-gamma NNP 2171 2173 on on IN 2174 2177 the the DT 2178 2188 expression expression NN 2189 2191 of of IN 2192 2195 the the DT 2196 2209 transcription transcription NN 2210 2217 factors factor NNS 2218 2222 AP-1 AP-1 NNP 2223 2226 and and CC 2227 2235 NF-kappa NF-kappa NNP 2236 2237 B B NNP 2238 2241 may may MD 2242 2244 be be VB 2245 2254 important important JJ 2255 2258 for for IN 2259 2262 the the DT 2263 2273 modulatory modulatory JJ 2274 2281 effects effect NNS 2282 2284 of of IN 2285 2294 IFN-gamma IFN-gamma NNP 2295 2297 on on IN 2298 2301 the the DT 2302 2310 cytokine cytokine NN 2311 2321 expression expression NN 2322 2324 in in IN 2325 2334 activated activate VBN 2335 2340 human human JJ 2341 2350 monocytes monocyte NNS 2350 2351 . . .