0 8 Abundant abundant JJ 9 19 expression expression NN 20 22 of of IN 23 32 erythroid erythroid JJ 33 46 transcription transcription NN 47 53 factor factor NN 54 57 P45 P45 NNP 58 63 NF-E2 NF-E2 NNP 64 68 mRNA mRNA NNP 69 71 in in IN 72 77 human human JJ 78 88 peripheral peripheral JJ 89 101 granurocytes granurocyte NNS 101 102 . . . 104 117 Transcription Transcription NNP 118 124 factor factor NN 125 130 NF-E2 nf-e2 NN 131 133 is be VBZ 134 141 crucial crucial JJ 142 145 for for IN 146 156 regulation regulation NN 157 159 of of IN 160 178 erythroid-specific erythroid-specific JJ 179 183 gene gene NN 184 195 expression. expression. NN 196 199 p45 p45 NN 200 207 subunit subunit NN 208 210 of of IN 211 216 NF-E2 nf-e2 NN 217 225 contains contain VBZ 226 227 a a DT 228 241 basic-leucine basic-leucine NN 242 248 zipper zipper NN 249 255 domain domain NN 256 259 and and CC 260 269 dimerizes dimerize VBZ 270 274 with with IN 275 278 the the DT 279 284 small small JJ 285 288 Maf Maf NNP 289 295 family family NN 296 303 protein protein NN 304 306 to to TO 307 311 form form VB 312 322 functional functional JJ 323 328 NF-E2 nf-e2 NN 329 336 complex complex NN 336 337 . . . 338 343 While while IN 344 347 p45 p45 NN 348 358 expression expression NN 359 362 was be VBD 363 368 shown show VBN 369 371 to to TO 372 374 be be VB 375 385 restricted restricted JJ 386 388 to to TO 389 398 erythroid erythroid JJ 399 404 cells cell NNS 404 405 , , , 406 420 megakaryocytes megakaryocyte NNS 421 424 and and CC 425 429 mast mast NN 430 435 cells cell NNS 436 438 in in IN 439 452 hematopoietic hematopoietic JJ 453 460 lineage lineage NN 460 461 , , , 462 464 we we PRP 465 470 found find VBD 471 473 in in IN 474 478 this this DT 479 484 study study NN 485 489 that that IN 490 493 p45 p45 NN 494 498 mRNA mrna NN 499 501 is be VBZ 502 512 abundantly abundantly RB 513 524 transcribed transcribe VBN 525 527 in in IN 528 531 the the DT 532 543 granulocyte granulocyte NN 544 552 fraction fraction NN 553 555 of of IN 556 561 human human JJ 562 572 peripheral peripheral JJ 573 578 blood blood NN 579 584 cells cell NNS 584 585 . . . 586 588 As as IN 589 600 neutrophils neutrophil NNS 601 607 occupy occupy VBP 608 621 approximately approximately RB 622 624 92 92 CD 624 625 % % NN 626 628 of of IN 629 632 the the DT 633 638 cells cell NNS 639 641 in in IN 642 653 granulocyte granulocyte NN 654 662 fraction fraction NN 663 665 of of IN 666 671 human human JJ 672 682 peripheral peripheral JJ 683 688 blood blood NN 689 694 cells cell NNS 694 695 . . . 696 698 As as IN 699 710 neutrophils neutrophil NNS 711 717 occupy occupy VBP 718 731 approximately approximately RB 732 734 92 92 CD 734 735 % % NN 736 738 of of IN 739 742 the the DT 743 748 cells cell NNS 749 751 in in IN 752 756 this this DT 757 765 fraction fraction NN 765 766 , , , 767 770 the the DT 771 776 cells cell NNS 777 787 expressing express VBG 788 791 p45 p45 NN 792 794 is be VBZ 795 799 most most RBS 800 806 likely likely JJ 807 809 to to TO 810 812 be be VB 813 825 neutrophils. neutrophils. JJ 826 829 p45 p45 NN 830 834 mRNA mRNA NNP 835 837 is be VBZ 838 842 also also RB 843 852 expressed express VBN 853 855 in in IN 856 861 HL-60 hl-60 NN 862 875 promyelocytes promyelocyte NNS 875 876 , , , 877 883 albeit albeit IN 884 887 the the DT 888 898 expression expression NN 899 904 level level NN 905 907 is be VBZ 908 912 much much RB 913 918 lower low JJR 919 923 than than IN 924 928 that that DT 929 931 of of IN 932 935 the the DT 936 947 granulocyte granulocyte NN 948 956 fraction fraction NN 956 957 . . . 958 963 HL-60 HL-60 NNP 964 969 cells cell NNS 970 974 were be VBD 975 980 found find VBN 981 983 to to TO 984 991 express express VB 992 996 mafK mafk NN 997 1001 mRNA mrna NN 1001 1002 , , , 1003 1013 indicating indicate VBG 1014 1017 the the DT 1018 1026 presence presence NN 1027 1029 of of IN 1030 1037 genuine genuine JJ 1038 1043 NF-E2 nf-e2 NN 1044 1051 complex complex NN 1052 1054 in in IN 1055 1058 the the DT 1059 1064 cells cell NNS 1064 1065 . . . 1066 1074 Although although IN 1075 1078 p45 p45 NN 1079 1083 mRNA mrna NN 1084 1086 is be VBZ 1087 1098 transcribed transcribe VBN 1099 1103 from from IN 1104 1107 two two CD 1108 1117 different different JJ 1118 1127 promoters promoter NNS 1127 1128 , , , 1129 1135 aNF-E2 anf-e2 NN 1136 1144 promoter promoter NN 1145 1148 and and CC 1149 1155 fNF-E2 fnf-e2 NN 1156 1164 promoter promoter NN 1164 1165 , , , 1166 1168 in in IN 1169 1178 erythroid erythroid JJ 1179 1182 and and CC 1183 1197 megakaryocytic megakaryocytic JJ 1198 1205 lineage lineage NN 1206 1211 cells cell NNS 1211 1212 , , , 1213 1216 p45 p45 NN 1217 1221 mRNA mRNA NNP 1222 1224 is be VBZ 1225 1236 transcribed transcribe VBN 1237 1241 only only RB 1242 1246 from from IN 1247 1253 aNF-E2 anf-e2 NN 1254 1262 promoter promoter NN 1262 1263 . . . 1264 1267 The the DT 1268 1278 expression expression NN 1279 1281 of of IN 1282 1285 p45 p45 NN 1286 1300 megakaryocytic megakaryocytic JJ 1301 1308 lineage lineage NN 1309 1314 cells cell NNS 1314 1315 , , , 1316 1319 p45 p45 NN 1320 1324 mRNA mRNA NNP 1325 1327 is be VBZ 1328 1339 transcribed transcribe VBN 1340 1344 only only RB 1345 1349 from from IN 1350 1356 aNF-E2 anf-e2 NN 1357 1365 promoter promoter NN 1365 1366 . . . 1367 1370 The the DT 1371 1381 expression expression NN 1382 1384 of of IN 1385 1388 p45 p45 NN 1389 1393 mRNA mrna NN 1394 1396 in in IN 1397 1400 the the DT 1401 1412 neutrophils neutrophil NNS 1413 1421 declined decline VBD 1422 1429 rapidly rapidly RB 1430 1435 after after IN 1436 1444 transfer transfer NN 1445 1447 of of IN 1448 1451 the the DT 1452 1457 cells cell NNS 1458 1460 to to TO 1461 1463 in in FW 1464 1469 vitro vitro FW 1470 1477 culture culture NN 1478 1481 and and CC 1482 1487 G-CSF G-CSF NNP 1488 1493 could could MD 1494 1497 not not RB 1498 1505 sustain sustain VB 1506 1509 the the DT 1510 1520 expression expression NN 1521 1525 from from IN 1526 1529 the the DT 1530 1545 down-regulation down-regulation NN 1545 1546 , , , 1547 1557 suggesting suggest VBG 1558 1561 the the DT 1562 1564 E2 e2 NN 1565 1568 may may MD 1569 1573 also also RB 1574 1585 participate participate VB 1586 1588 in in IN 1589 1592 the the DT 1593 1603 regulation regulation NN 1604 1606 of of IN 1607 1626 neutrophil-specific neutrophil-specific JJ 1627 1631 gene gene NN 1632 1642 expression expression NN 1642 1643 . . .