0 8 Multiple multiple JJ 9 22 transcription transcription NN 23 30 factors factor NNS 31 34 are be VBP 35 43 required require VBN 44 47 for for IN 48 58 activation activation NN 59 61 of of IN 62 67 human human JJ 68 79 interleukin interleukin NN 80 81 9 9 CD 82 86 gene gene NN 87 89 in in IN 90 91 T t NN 92 97 cells cell NNS 97 98 . . . 100 103 The the DT 104 111 genetic genetic JJ 112 120 elements element NNS 121 124 and and CC 125 135 regulatory regulatory JJ 136 146 mechanisms mechanism NNS 147 158 responsible responsible JJ 159 162 for for IN 163 168 human human JJ 169 180 interleukin interleukin NN 181 182 9 9 CD 183 184 ( ( ( 184 188 IL-9 IL-9 NNP 188 189 ) ) ) 190 194 gene gene NN 195 205 expression expression NN 206 208 in in IN 209 210 a a DT 211 216 human human JJ 217 218 T t NN 219 223 cell cell NN 224 232 leukemia leukemia NN 233 238 virus virus NN 239 243 type type NN 244 257 I-transformed i-transformed JJ 258 263 human human JJ 264 265 T t NN 266 270 cell cell NN 271 275 line line NN 275 276 , , , 277 282 C5MJ2 C5MJ2 NNP 282 283 , , , 284 288 were be VBD 289 301 investigated investigate VBN 301 302 . . . 303 305 We we PRP 306 318 demonstrated demonstrate VBD 319 323 that that IN 324 328 IL-9 IL-9 NNP 329 333 gene gene NN 334 344 expression expression NN 345 347 is be VBZ 348 358 controlled control VBN 358 359 , , , 360 362 at at IN 363 368 least least JJS 369 371 in in IN 372 376 part part NN 376 377 , , , 378 380 by by IN 381 396 transcriptional transcriptional JJ 397 407 activation activation NN 407 408 . . . 409 418 Transient transient JJ 419 429 expression expression NN 430 432 of of IN 433 436 the the DT 437 447 luciferase luciferase NN 448 456 reporter reporter NN 457 461 gene gene NN 462 468 linked link VBN 469 471 to to TO 472 480 serially serially RB 481 488 deleted delete VBN 489 498 sequences sequence NNS 499 501 of of IN 502 505 the the DT 506 517 5’-flanking 5'-flanking JJ 518 524 region region NN 525 527 of of IN 528 531 the the DT 532 536 IL-9 IL-9 NNP 537 541 gene gene NN 542 545 has have VBZ 546 554 revealed reveal VBN 555 562 several several JJ 563 571 positive positive JJ 572 575 and and CC 576 584 negative negative JJ 585 595 regulatory regulatory JJ 596 604 elements element NNS 605 613 involved involve VBN 614 616 in in IN 617 620 the the DT 621 626 basal basal JJ 627 630 and and CC 631 640 inducible inducible JJ 641 651 expression expression NN 652 654 of of IN 655 658 the the DT 659 663 IL-9 IL-9 NNP 664 668 gene gene NN 669 671 in in IN 672 677 C5MJ2 c5mj2 NN 678 683 cells cell NNS 683 684 . . . 685 687 An an DT 688 692 AP-1 ap-1 NN 693 697 site site NN 698 700 at at IN 701 705 -146 -146 CD 706 708 to to TO 709 713 -140 -140 CD 714 717 was be VBD 718 723 shown show VBN 724 726 to to TO 727 729 be be VB 730 738 involved involve VBN 739 741 in in IN 742 745 the the DT 746 756 expression expression NN 757 759 of of IN 760 763 the the DT 764 768 IL-9 IL-9 NNP 769 773 gene gene NN 773 774 . . . 775 776 A a DT 777 785 proximal proximal JJ 786 792 region region NN 793 800 between between IN 801 804 -46 -46 CD 805 808 and and CC 809 812 -80 -80 CD 813 816 was be VBD 817 827 identified identify VBN 828 830 as as IN 831 834 the the DT 835 842 minimum minimum JJ 843 851 sequence sequence NN 852 855 for for IN 856 859 the the DT 860 865 basal basal JJ 866 869 and and CC 870 879 inducible inducible JJ 880 890 expression expression NN 891 893 of of IN 894 897 the the DT 898 902 IL-9 IL-9 NNP 903 907 gene gene NN 908 910 in in IN 911 916 C5MJ2 c5mj2 NN 917 922 cells cell NNS 922 923 . . . 924 930 Within within IN 931 935 this this DT 936 942 region region NN 942 943 , , , 944 946 an an DT 947 956 NF-kappaB NF-kappaB NNP 957 961 site site NN 962 964 at at IN 965 968 -59 -59 CD 969 971 to to TO 972 975 -50 -50 CD 976 979 and and CC 980 983 its its PRP$ 984 992 adjacent adjacent JJ 993 1000 20-base 20-base JJ 1001 1005 pair pair NN 1006 1014 upstream upstream JJ 1015 1023 sequence sequence NN 1024 1028 were be VBD 1029 1041 demonstrated demonstrate VBN 1042 1044 to to TO 1045 1049 play play VB 1050 1051 a a DT 1052 1060 critical critical JJ 1061 1065 role role NN 1066 1069 for for IN 1070 1073 the the DT 1074 1078 IL-9 IL-9 NNP 1079 1087 promoter promoter NN 1088 1096 activity activity NN 1096 1097 . . . 1098 1109 DNA-protein dna-protein JJ 1110 1117 binding binding NN 1118 1125 studies study NNS 1126 1135 indicated indicate VBD 1136 1140 that that IN 1141 1150 NF-kappaB NF-kappaB NNP 1150 1151 , , , 1152 1157 c-Jun c-jun NN 1157 1158 , , , 1159 1162 and and CC 1163 1174 potentially potentially RB 1175 1180 novel novel JJ 1181 1189 proteins protein NNS 1190 1191 ( ( ( 1191 1197 around around IN 1198 1200 35 35 CD 1201 1204 kDa kda NN 1204 1205 ) ) ) 1206 1209 can can MD 1210 1214 bind bind VB 1215 1217 to to TO 1218 1222 this this DT 1223 1232 important important JJ 1233 1241 sequence sequence NN 1241 1242 . . . 1243 1252 Mutations mutation NNS 1253 1255 at at IN 1256 1265 different different JJ 1266 1271 sites site NNS 1272 1278 within within IN 1279 1283 this this DT 1284 1292 proximal proximal JJ 1293 1301 promoter promoter NN 1302 1308 region region NN 1309 1318 abolished abolish VBD 1319 1322 the the DT 1323 1331 promoter promoter NN 1332 1340 activity activity NN 1341 1343 as as RB 1344 1348 well well RB 1349 1351 as as IN 1352 1355 the the DT 1356 1359 DNA dna NN 1360 1367 binding binding NN 1367 1368 . . . 1369 1374 Taken take VBN 1375 1383 together together RB 1383 1384 , , , 1385 1390 these these DT 1391 1398 results result NNS 1399 1406 suggest suggest VBP 1407 1411 that that IN 1412 1415 the the DT 1416 1427 cooperation cooperation NN 1428 1430 of of IN 1431 1440 different different JJ 1441 1454 transcription transcription NN 1455 1462 factors factor NNS 1463 1465 is be VBZ 1466 1475 essential essential JJ 1476 1479 for for IN 1480 1484 IL-9 IL-9 NNP 1485 1489 gene gene NN 1490 1500 expression expression NN 1501 1503 in in IN 1504 1505 T t NN 1506 1511 cells cell NNS 1511 1512 . . .