0 7 Mapping mapping NN 8 10 of of IN 11 14 the the DT 15 30 transcriptional transcriptional JJ 31 41 repression repression NN 42 48 domain domain NN 49 51 of of IN 52 55 the the DT 56 73 lymphoid-specific lymphoid-specific JJ 74 87 transcription transcription NN 88 94 factor factor NN 95 101 oct-2A oct-2A NNP 101 102 . . . 104 107 The the DT 108 125 lymphoid-specific lymphoid-specific JJ 126 139 transcription transcription NN 140 146 factor factor NN 147 153 Oct-2a Oct-2a NNP 154 156 is be VBZ 157 167 implicated implicate VBN 168 170 in in IN 171 172 B B NNP 173 186 cell-specific cell-specific JJ 187 202 transcriptional transcriptional JJ 203 211 activity activity NN 212 215 via via IN 216 219 the the DT 220 227 octamer octamer NN 228 233 motif motif NN 233 234 . . . 235 253 Structure/function Structure/function NNP 254 262 analysis analysis NN 263 265 of of IN 266 273 various various JJ 274 280 Oct-2a Oct-2a NNP 281 289 effector effector NN 290 297 regions region NNS 298 300 in in IN 301 304 the the DT 305 312 context context NN 313 315 of of IN 316 319 the the DT 320 324 GAL4 gal4 NN 325 336 DNA-binding dna-binding JJ 337 343 domain domain NN 344 352 revealed reveal VBD 353 357 that that IN 358 364 Oct-2a Oct-2a NNP 365 373 contains contain VBZ 374 377 two two CD 378 390 functionally functionally RB 391 400 different different JJ 401 411 activation activation NN 412 419 domains domain NNS 420 422 at at IN 423 426 the the DT 427 428 N N NNP 429 432 and and CC 433 436 the the DT 437 438 C C NNP 439 446 termini terminus NNS 446 447 . . . 448 451 The the DT 452 467 transcriptional transcriptional JJ 468 476 activity activity NN 477 479 of of IN 480 484 both both DT 485 492 domains domain NNS 493 495 is be VBZ 496 504 strongly strongly RB 505 516 potentiated potentiate VBN 517 519 by by IN 520 532 interactions interaction NNS 533 537 with with IN 538 546 distinct distinct JJ 547 548 B B NNP 549 562 cell-specific cell-specific JJ 563 575 coactivators coactivator NNS 575 576 . . . 577 585 Recently recently RB 585 586 , , , 587 589 we we PRP 590 594 have have VBP 595 605 identified identify VBN 606 607 a a DT 608 618 repression repression NN 619 625 domain domain NN 626 633 located located JJ 634 640 within within IN 641 644 the the DT 645 646 N N NNP 647 655 terminus terminus NN 656 658 of of IN 659 665 Oct-2a Oct-2a NNP 666 667 ( ( ( 667 672 amino amino NN 673 678 acids acid NNS 679 683 2-99 2-99 CD 683 684 ) ) ) 684 685 . . . 686 690 When when WRB 691 695 this this DT 696 702 domain domain NN 703 706 was be VBD 707 718 transferred transfer VBN 719 721 to to TO 722 723 a a DT 724 730 potent potent JJ 731 740 activator activator NN 740 741 , , , 742 755 transcription transcription NN 756 759 was be VBD 760 768 strongly strongly RB 769 778 inhibited inhibit VBN 778 779 . . . 780 782 In in IN 783 787 this this DT 788 793 study study NN 794 796 we we PRP 797 804 present present VBP 805 806 a a DT 807 815 deletion deletion NN 816 824 analysis analysis NN 825 827 of of IN 828 831 the the DT 832 842 N-terminal n-terminal JJ 843 849 region region NN 850 852 of of IN 853 859 Oct-2a Oct-2a NNP 860 862 to to TO 863 872 determine determine VB 873 876 the the DT 877 884 minimal minimal JJ 885 895 repression repression NN 896 902 domain domain NN 902 903 . . . 904 906 We we PRP 907 917 identified identify VBD 918 919 a a DT 920 927 stretch stretch NN 928 930 of of IN 931 933 23 23 CD 934 939 amino amino NN 940 945 acids acid NNS 945 946 , , , 947 951 rich rich JJ 952 954 in in IN 955 961 serine serine NN 962 965 and and CC 966 975 threonine threonine NN 976 984 residues residue NNS 984 985 , , , 986 991 which which WDT 992 995 was be VBD 996 1007 responsible responsible JJ 1008 1011 for for IN 1012 1016 most most JJS 1017 1019 of of IN 1020 1023 the the DT 1024 1034 repression repression NN 1035 1043 activity activity NN 1043 1044 . . . 1045 1047 We we PRP 1048 1052 show show VBP 1053 1057 that that IN 1058 1068 repression repression NN 1069 1071 is be VBZ 1072 1080 strongly strongly RB 1081 1090 dependent dependent JJ 1091 1093 on on IN 1094 1097 the the DT 1098 1102 type type NN 1103 1105 of of IN 1106 1114 enhancer enhancer NN 1115 1122 present present JJ 1123 1125 in in IN 1126 1129 the the DT 1130 1138 reporter reporter NN 1139 1146 plasmid plasmid NN 1147 1149 as as RB 1150 1154 well well RB 1155 1157 as as IN 1158 1160 on on IN 1161 1164 the the DT 1165 1169 cell cell NN 1170 1174 line line NN 1175 1181 tested test VBN 1181 1182 . . . 1183 1186 The the DT 1187 1198 possibility possibility NN 1199 1203 that that IN 1204 1210 Oct-2a Oct-2a NNP 1211 1214 can can MD 1215 1218 act act VB 1219 1221 as as IN 1222 1224 an an DT 1225 1234 activator activator NN 1235 1241 and/or and/or CC 1242 1243 a a DT 1244 1253 repressor repressor NN 1254 1257 may may MD 1258 1262 have have VB 1263 1272 important important JJ 1273 1285 consequences consequence NNS 1286 1289 for for IN 1290 1293 the the DT 1294 1302 function function NN 1303 1305 of of IN 1306 1312 Oct-2a Oct-2a NNP 1313 1315 in in IN 1316 1317 B B NNP 1318 1322 cell cell NN 1323 1338 differentiation differentiation NN 1339 1342 and and CC 1343 1348 other other JJ 1349 1362 developmental developmental JJ 1363 1372 processes process NNS 1372 1373 . . .