0 10 Activation Activation NNP 11 18 protein protein NN 19 30 1-dependent 1-dependent JJ 31 46 transcriptional transcriptional JJ 47 57 activation activation NN 58 60 of of IN 61 72 interleukin interleukin NN 73 74 2 2 CD 75 79 gene gene NN 80 82 by by IN 83 98 Ca2+/calmodulin Ca2+/calmodulin NNP 99 105 kinase kinase NNP 106 110 type type NN 111 117 IV/Gr. IV/Gr. NNP 119 122 The the DT 123 148 Ca2+/calmodulin-dependent ca2+/calmodulin-dependent JJ 149 156 protein protein NN 157 163 kinase kinase NNP 164 165 ( ( ( 165 169 CaMK CaMK NNP 169 170 ) ) ) 171 175 type type NN 176 181 IV/Gr IV/Gr NNP 182 184 is be VBZ 185 196 selectively selectively RB 197 206 expressed express VBN 207 209 in in IN 210 211 T t NN 212 223 lymphocytes lymphocyte NNS 224 227 and and CC 228 230 is be VBZ 231 240 activated activate VBN 241 246 after after IN 247 256 signaling signal VBG 257 260 via via IN 261 264 the the DT 265 266 T t NN 267 271 cell cell NN 272 279 antigen antigen NN 280 288 receptor receptor NN 289 290 ( ( ( 290 293 TCR TCR NNP 293 294 ) ) ) 294 295 , , , 296 306 indicating indicate VBG 307 311 that that IN 312 314 it it PRP 315 323 mediates mediate VBZ 324 328 some some DT 329 331 of of IN 332 335 the the DT 336 352 Ca(2+)-dependent ca(2+)-dependent JJ 353 368 transcriptional transcriptional JJ 369 375 events event NNS 376 380 that that WDT 381 387 follow follow VBP 388 391 TCR TCR NNP 392 402 engagement engagement NN 402 403 . . . 404 408 Here here RB 409 411 we we PRP 412 416 show show VBP 417 421 that that IN 422 431 CaMKIV/Gr CaMKIV/Gr NNP 432 439 induces induce VBZ 440 443 the the DT 444 457 transcription transcription NN 458 464 factor factor NN 465 475 activation activation NN 476 483 protein protein NN 484 485 1 1 CD 486 487 ( ( ( 487 491 AP-1 ap-1 NN 491 492 ) ) ) 493 498 alone alone RB 499 501 or or CC 502 504 in in IN 505 512 synergy synergy NN 513 517 with with IN 518 519 T t NN 520 524 cell cell NN 525 533 mitogens mitogen NNS 534 537 and and CC 538 542 with with IN 543 546 the the DT 547 553 p21ras p21ras NN 554 565 oncoprotein oncoprotein NN 565 566 . . . 567 574 CaMKIV/ CaMKIV/ NNP 575 577 Gr Gr NNP 578 587 signaling signaling NN 588 590 is be VBZ 591 601 associated associate VBN 602 606 with with IN 607 622 transcriptional transcriptional JJ 623 633 activation activation NN 634 636 of of IN 637 642 c-fos c-fos NN 643 646 but but CC 647 649 is be VBZ 650 661 independent independent JJ 662 664 of of IN 665 671 p21ras p21ras NN 672 674 or or CC 675 686 calcineurin calcineurin NN 686 687 . . . 688 692 AP-1 ap-1 NN 693 695 is be VBZ 696 698 an an DT 699 707 integral integral JJ 708 717 component component NN 718 720 of of IN 721 724 the the DT 725 732 nuclear nuclear JJ 733 739 factor factor NN 740 742 of of IN 743 752 activated activate VBN 753 754 T t NN 755 760 cells cell NNS 761 762 ( ( ( 762 766 NFAT NFAT NNP 766 767 ) ) ) 768 783 transcriptional transcriptional JJ 784 791 complex complex NN 791 792 , , , 793 798 which which WDT 799 801 is be VBZ 802 810 required require VBN 811 814 for for IN 815 826 interleukin interleukin NN 827 828 2 2 CD 829 833 gene gene NN 834 844 expression expression NN 845 847 in in IN 848 849 T t NN 850 855 cells cell NNS 855 856 . . . 857 859 We we PRP 860 871 demonstrate demonstrate VBP 872 876 that that IN 877 886 CaMKIV/Gr CaMKIV/Gr NNP 887 900 reconstitutes reconstitute VBZ 901 904 the the DT 905 913 capacity capacity NN 914 916 of of IN 917 920 the the DT 921 930 cytosolic cytosolic JJ 931 940 component component NN 941 943 of of IN 944 948 NFAT NFAT NNP 949 951 to to TO 952 958 direct direct VB 959 972 transcription transcription NN 973 977 from from IN 978 982 NFAT nfat NN 983 988 sites site NNS 989 991 in in IN 992 997 non-T non-t JJ 998 1003 cells cell NNS 1003 1004 . . . 1005 1010 These these DT 1011 1018 results result NNS 1019 1025 reveal reveal VBP 1026 1027 a a DT 1028 1035 central central JJ 1036 1040 role role NN 1041 1044 for for IN 1045 1054 CaMKIV/Gr CaMKIV/Gr NNP 1055 1057 as as IN 1058 1059 a a DT 1060 1076 Ca(2+)-regulated ca(2+)-regulated JJ 1077 1086 activator activator NN 1087 1089 of of IN 1090 1094 gene gene NN 1095 1108 transcription transcription NN 1109 1111 in in IN 1112 1113 T t NN 1114 1125 lymphocytes lymphocyte NNS 1125 1126 . . .