0 10 Mechanisms Mechanism NNPS 11 13 of of IN 14 29 transactivation transactivation NN 30 32 by by IN 33 40 nuclear nuclear JJ 41 47 factor factor NN 48 50 of of IN 51 60 activated activate VBN 61 62 T t NN 63 70 cells-1 cells-1 NNS 70 71 . . . 73 80 Nuclear nuclear JJ 81 87 factor factor NN 88 90 of of IN 91 100 activated activate VBN 101 102 T t NN 103 115 cells-family cells-family NN 116 124 proteins protein NNS 125 126 ( ( ( 126 137 NFAT1/NFATp NFAT1/NFATp NNP 137 138 , , , 139 144 NFATc NFATc NNP 144 145 , , , 146 151 NFAT3 NFAT3 NNP 151 152 , , , 153 156 and and CC 157 175 NFAT4/NFATx/NFATc3 NFAT4/NFATx/NFATc3 NNP 175 176 ) ) ) 177 181 play play VBP 182 183 a a DT 184 187 key key JJ 188 192 role role NN 193 195 in in IN 196 199 the the DT 200 213 transcription transcription NN 214 216 of of IN 217 225 cytokine cytokine NN 226 231 genes gene NNS 232 235 and and CC 236 241 other other JJ 242 247 genes gene NNS 248 254 during during IN 255 258 the the DT 259 265 immune immune JJ 266 274 response response NN 274 275 . . . 276 278 We we PRP 279 283 have have VBP 284 291 defined define VBN 292 295 the the DT 296 306 mechanisms mechanism NNS 307 309 of of IN 310 325 transactivation transactivation NN 326 328 by by IN 329 334 NFAT1 NFAT1 NNP 334 335 . . . 336 341 NFAT1 NFAT1 NNP 342 351 possesses possess VBZ 352 355 two two CD 356 371 transactivation transactivation NN 372 379 domains domain NNS 380 385 whose whose WP$ 386 395 sequences sequence NNS 396 399 are be VBP 400 403 not not RB 404 413 conserved conserve VBN 414 416 in in IN 417 420 the the DT 421 426 other other JJ 427 438 NFAT-family NFAT-family NNP 439 447 proteins protein NNS 447 448 , , , 449 452 and and CC 453 454 a a DT 455 464 conserved conserve VBN 465 476 DNA-binding dna-binding JJ 477 483 domain domain NN 484 488 that that WDT 489 497 mediates mediate VBZ 498 501 the the DT 502 513 recruitment recruitment NN 514 516 of of IN 517 528 cooperating cooperate VBG 529 536 nuclear nuclear JJ 537 550 transcription transcription NN 551 558 factors factor NNS 559 563 even even RB 564 568 when when WRB 569 571 it it PRP 572 574 is be VBZ 575 584 expressed express VBN 585 587 in in IN 588 591 the the DT 592 599 absence absence NN 600 602 of of IN 603 608 other other JJ 609 616 regions region NNS 617 619 of of IN 620 623 the the DT 624 631 protein protein NN 631 632 . . . 633 636 The the DT 637 645 activity activity NN 646 648 of of IN 649 652 the the DT 653 665 NH2-terminal nh2-terminal JJ 666 681 transactivation transactivation NN 682 688 domain domain NN 689 691 is be VBZ 692 701 modulated modulate VBN 702 704 by by IN 705 707 an an DT 708 716 adjacent adjacent JJ 717 727 regulatory regulatory JJ 728 734 region region NN 735 739 that that WDT 740 748 contains contain VBZ 749 756 several several JJ 757 766 conserved conserve VBN 767 775 sequence sequence NN 776 782 motifs motif NNS 783 794 represented represent VBD 795 799 only only RB 800 802 in in IN 803 806 the the DT 807 811 NFAT NFAT NNP 812 818 family family NN 818 819 . . . 820 823 Our our PRP$ 824 831 results result NNS 832 841 emphasize emphasize VBP 842 845 the the DT 846 854 multiple multiple JJ 855 861 levels level NNS 862 864 at at IN 865 870 which which WDT 871 885 NFAT-dependent nfat-dependent JJ 886 901 transactivation transactivation NN 902 904 is be VBZ 905 914 regulated regulate VBN 914 915 , , , 916 919 and and CC 920 927 predict predict VBP 928 939 significant significant JJ 940 951 differences difference NNS 952 954 in in IN 955 958 the the DT 959 971 architecture architecture NN 972 974 of of IN 975 986 cooperative cooperative JJ 987 1000 transcription transcription NN 1001 1010 complexes complex NNS 1011 1021 containing contain VBG 1022 1031 different different JJ 1032 1043 NFAT-family NFAT-family NNP 1044 1052 proteins protein NNS 1052 1053 . . .