0 16 Characterization characterization NN 17 19 of of IN 20 21 a a DT 22 25 new new JJ 26 33 isoform isoform NN 34 36 of of IN 37 40 the the DT 41 45 NFAT NFAT NNP 46 47 ( ( ( 47 54 nuclear nuclear JJ 55 61 factor factor NN 62 64 of of IN 65 74 activated activate VBN 75 76 T t NN 77 82 cells cell NNS 82 83 ) ) ) 84 88 gene gene NN 89 95 family family NN 96 102 member member NN 103 108 NFATc NFATc NNP 109 110 [ [ ( 110 119 published publish VBN 120 127 erratum erratum NN 128 135 appears appear VBZ 136 138 in in IN 139 140 J J NNP 141 145 Biol Biol NNP 146 150 Chem Chem NNP 151 155 1996 1996 CD 156 159 Dec Dec NNP 160 176 27;271(52):33705 27;271(52):33705 CD 176 177 ] ] ) 179 182 The the DT 183 194 cyclosporin cyclosporin NN 195 196 A a NN 197 198 ( ( ( 198 218 CsA)/FK506-sensitive csa)/fk506-sensitive JJ 219 226 nuclear nuclear JJ 227 233 factor factor NN 234 236 of of IN 237 246 activated activate VBN 247 248 T t NN 249 254 cells cell NNS 255 256 ( ( ( 256 260 NFAT NFAT NNP 260 261 ) ) ) 262 267 plays play VBZ 268 269 a a DT 270 273 key key JJ 274 278 role role NN 279 281 in in IN 282 285 the the DT 286 295 inducible inducible JJ 296 306 expression expression NN 307 309 of of IN 310 318 cytokine cytokine NN 319 324 genes gene NNS 325 327 in in IN 328 329 T t NN 330 335 cells cell NNS 335 336 . . . 337 345 Although although IN 346 350 NFAT NFAT NNP 351 354 has have VBZ 355 359 been be VBN 360 368 recently recently RB 369 374 shown show VBN 375 377 to to TO 378 380 be be VB 381 390 inducible inducible JJ 391 393 in in IN 394 401 several several JJ 402 407 non-T non-t JJ 408 414 immune immune JJ 415 420 cells cell NNS 420 421 , , , 422 425 the the DT 426 430 NFAT NFAT NNP 431 435 gene gene NN 436 442 family family NN 443 450 members member NNS 451 464 characterized characterize VBN 465 467 to to TO 468 472 date date NN 473 477 have have VBP 478 482 been be VBN 483 491 isolated isolate VBN 492 496 only only RB 497 501 from from IN 502 503 T t NN 504 509 cells cell NNS 509 510 . . . 511 513 To to TO 514 521 further further RBR 522 534 characterize characterize VB 535 539 NFAT nfat NN 540 548 function function NN 549 551 in in IN 552 557 human human JJ 558 559 B B NNP 560 565 cells cell NNS 566 569 and and CC 570 572 to to TO 573 584 demonstrate demonstrate VB 585 593 cytokine cytokine NN 594 598 gene gene NN 599 610 specificity specificity NN 611 613 of of IN 614 618 NFAT NFAT NNP 619 627 proteins protein NNS 627 628 , , , 629 631 we we PRP 632 638 report report VBP 639 643 here here RB 644 647 the the DT 648 657 isolation isolation NN 658 661 and and CC 662 678 characterization characterization NN 679 681 of of IN 682 683 a a DT 684 688 cDNA cdna NN 689 694 clone clone NN 695 699 from from IN 700 703 the the DT 704 708 Raji Raji NNP 709 710 B B NNP 711 715 cell cell NN 716 720 line line NN 720 721 . . . 722 725 The the DT 726 730 cDNA cdna NN 731 736 clone clone NN 737 744 encodes encode VBZ 745 746 a a DT 747 750 new new JJ 751 758 isoform isoform NN 758 759 , , , 760 770 NFATc.beta NFATc.beta NNP 770 771 , , , 772 774 of of IN 775 778 the the DT 779 783 NFAT NFAT NNP 784 788 gene gene NN 789 795 family family NN 796 802 member member NN 803 808 NFATc NFATc NNP 809 810 ( ( ( 810 820 designated designate VBN 821 825 here here RB 826 837 NFATc.alpha NFATc.alpha NNP 837 838 ) ) ) 838 839 . . . 840 843 The the DT 844 849 amino amino JJ 850 854 acid acid NN 855 863 sequence sequence NN 864 866 of of IN 867 877 NFATc.beta NFATc.beta NNP 878 885 differs differ VBZ 886 890 from from IN 891 895 that that DT 896 898 of of IN 899 910 NFATc.alpha NFATc.alpha NNP 911 913 in in IN 914 917 the the DT 918 923 first first JJ 924 936 NH2-terminal nh2-terminal JJ 937 939 29 29 CD 940 948 residues residue NNS 949 952 and and CC 953 961 contains contain VBZ 962 964 an an DT 965 975 additional additional JJ 976 982 region region NN 983 985 of of IN 986 989 142 142 CD 990 998 residues residue NNS 999 1001 at at IN 1002 1005 the the DT 1006 1010 COOH COOH NNP 1011 1019 terminus terminus NN 1019 1020 . . . 1021 1029 Northern northern NN 1030 1038 analysis analysis NN 1039 1044 using use VBG 1045 1046 a a DT 1047 1052 probe probe NN 1053 1065 encompassing encompass VBG 1066 1067 a a DT 1068 1074 common common JJ 1075 1081 region region NN 1082 1084 of of IN 1085 1089 both both DT 1090 1098 isoforms isoform NNS 1099 1105 showed show VBD 1106 1109 two two CD 1110 1114 mRNA mrna NN 1115 1122 species specie NNS 1123 1125 of of IN 1126 1129 2.7 2.7 CD 1130 1133 and and CC 1134 1137 4.5 4.5 CD 1138 1146 kilobase kilobase NN 1147 1152 pairs pair NNS 1152 1153 , , , 1154 1159 while while IN 1160 1162 an an DT 1163 1182 NFATc.beta-specific nfatc.beta-specific JJ 1183 1188 probe probe NN 1189 1197 detected detect VBD 1198 1202 only only RB 1203 1206 the the DT 1207 1219 4.5-kilobase 4.5-kilobase JJ 1220 1224 pair pair NN 1225 1229 mRNA mrna NN 1230 1235 which which WDT 1236 1239 was be VBD 1240 1254 preferentially preferentially RB 1255 1264 expressed express VBN 1265 1267 in in IN 1268 1271 the the DT 1272 1278 spleen spleen NN 1278 1279 . . . 1280 1289 Transient transient JJ 1290 1300 expression expression NN 1301 1303 of of IN 1304 1314 NFATc.beta NFATc.beta NNP 1315 1318 was be VBD 1319 1326 capable capable JJ 1327 1329 of of IN 1330 1340 activating activate VBG 1341 1343 an an DT 1344 1357 interleukin-2 interleukin-2 NN 1358 1369 NFAT-driven nfat-driven JJ 1370 1378 reporter reporter NN 1379 1383 gene gene NN 1384 1386 in in IN 1387 1397 stimulated stimulate VBN 1398 1404 Jurkat jurkat NN 1405 1410 cells cell NNS 1411 1413 in in IN 1414 1415 a a DT 1416 1429 CsA-sensitive csa-sensitive JJ 1430 1436 manner manner NN 1436 1437 . . . 1438 1445 However however RB 1445 1446 , , , 1447 1457 NFATc.beta NFATc.beta NNP 1458 1465 neither neither CC 1466 1471 bound bind VBD 1472 1474 to to TO 1475 1478 the the DT 1479 1485 kappa3 kappa3 NN 1486 1493 element element NN 1494 1495 ( ( ( 1496 1498 an an DT 1499 1511 NFAT-binding nfat-binding JJ 1512 1516 site site NN 1517 1518 ) ) ) 1519 1521 in in IN 1522 1525 the the DT 1526 1531 tumor tumor NN 1532 1540 necrosis necrosis NN 1541 1553 factor-alpha factor-alpha NN 1554 1562 promoter promoter NN 1563 1566 nor nor CC 1567 1576 activated activate VBD 1577 1580 the the DT 1581 1586 tumor tumor NN 1587 1595 necrosis necrosis NN 1596 1608 factor-alpha factor-alpha NN 1609 1617 promoter promoter NN 1618 1620 in in IN 1621 1635 cotransfection cotransfection NN 1636 1642 assays assay NNS 1642 1643 . . . 1644 1649 These these DT 1650 1654 data datum NNS 1655 1662 suggest suggest VBP 1663 1667 that that IN 1668 1677 different different JJ 1678 1685 members member NNS 1686 1688 or or CC 1689 1697 isoforms isoform NNS 1698 1700 of of IN 1701 1705 NFAT NFAT NNP 1706 1710 gene gene NN 1711 1717 family family NN 1718 1721 may may MD 1722 1730 regulate regulate VB 1731 1740 inducible inducible JJ 1741 1751 expression expression NN 1752 1754 of of IN 1755 1764 different different JJ 1765 1773 cytokine cytokine NN 1774 1779 genes gene NNS 1779 1780 . . .