0 13 Transcription Transcription NNP 14 22 specific specific JJ 23 34 differences difference NNS 35 45 visualized visualize VBN 46 48 by by IN 49 61 fluorescence fluorescence NN 62 64 in in FW 65 69 situ situ FW 70 83 hybridization hybridization NN 84 91 pattern pattern NN 92 94 on on IN 95 105 interphase interphase NN 106 112 nuclei nucleus NNS 113 115 of of IN 116 125 different different JJ 126 130 cell cell NN 131 136 types type NNS 136 137 . . . 139 150 Application application NN 151 153 of of IN 154 155 a a DT 156 157 “ `` `` 157 166 formamide formamide NN 167 171 free free JJ 171 172 ” '' '' 173 176 and and CC 177 181 thus thus RB 182 183 “ `` `` 183 191 material material NN 192 202 preserving preserve VBG 202 203 ” '' '' 204 206 in in FW 207 211 situ situ FW 212 225 hybridization hybridization NN 226 235 technique technique NN 236 241 using use VBG 242 245 the the DT 246 250 cDNA cdna NN 251 253 of of IN 254 257 the the DT 258 262 myf3 myf3 NN 263 267 gene gene NN 268 276 revealed reveal VBD 277 280 the the DT 281 290 following following JJ 291 298 results result NNS 298 299 : : : 300 305 Human human JJ 306 322 rhabdomyosarcoma rhabdomyosarcoma NN 323 328 cells cell NNS 328 329 , , , 330 343 characterized characterize VBN 344 346 by by IN 347 348 a a DT 349 353 high high JJ 354 364 expression expression NN 365 367 of of IN 368 372 myf3 myf3 NN 373 377 show show VBP 378 387 intensive intensive JJ 388 401 hybridization hybridization NN 402 409 signals signal NNS 410 412 in in IN 413 418 their their PRP$ 419 429 interphase interphase NN 429 430 . . . 431 436 RNase RNase NNP 437 446 treatment treatment NN 447 452 prior prior JJ 453 455 to to TO 456 469 hybridization hybridization NN 470 482 considerably considerably RB 483 490 reduces reduce VBZ 491 494 the the DT 495 499 size size NN 500 502 of of IN 503 507 this this DT 508 515 signals signal NNS 515 516 . . . 517 519 In in IN 520 530 comparison comparison NN 530 531 , , , 532 540 isolated isolate VBN 541 547 nuclei nucleus NNS 548 550 of of IN 551 556 human human JJ 557 568 lymphocytes lymphocyte NNS 569 571 in in IN 572 577 which which WDT 578 580 no no DT 581 585 need need NN 586 589 for for IN 590 593 the the DT 594 604 expression expression NN 605 607 of of IN 608 612 this this DT 613 617 gene gene NN 618 624 exists exist VBZ 624 625 , , , 626 630 show show VBP 631 637 barely barely RB 638 651 hybridization hybridization NN 652 659 signals signal NNS 659 660 . . . 661 676 Correspondingly correspondingly RB 676 677 , , , 678 683 RNase RNase NNP 684 693 treatment treatment NN 694 697 had have VBD 698 700 no no DT 701 707 effect effect NN 708 710 on on IN 711 724 hybridization hybridization NN 725 732 pattern pattern NN 733 735 at at IN 736 739 all all DT 739 740 . . . 741 743 In in IN 744 754 conclusion conclusion NN 755 757 an an DT 758 767 increased increase VBN 768 781 transcription transcription NN 782 792 efficiency efficiency NN 793 795 of of IN 796 797 a a DT 798 802 cell cell NN 803 807 type type NN 808 816 specific specific JJ 817 821 gene gene NN 822 824 is be VBZ 825 836 accompanied accompany VBN 837 839 by by IN 840 841 a a DT 842 848 higher high JJR 849 862 hybridization hybridization NN 863 876 accessibility accessibility NN 877 879 in in IN 880 883 the the DT 884 897 corresponding correspond VBG 898 902 cell cell NN 903 909 nuclei nucleus NNS 909 910 . . .